scholarly journals Paired design of ATP bioluminescence method and colony counting method: whether it is suitable for evaluating the disinfection effect of environmental surface?

2019 ◽  
Author(s):  
Huiqiong Xu ◽  
Jiansheng Liang ◽  
Yimei Wang ◽  
Bin Wang ◽  
Tianbao Zhang ◽  
...  
Keyword(s):  
Rank Correlation ◽  
Correlation Coefficients ◽  
Atp Content ◽  
Counting Method ◽  
Colony Counting ◽  
Significant Difference ◽  
Atp Bioluminescence ◽  
Environmental Surface ◽  
Paired Design ◽  
Bioluminescence Method

AbstractATP bioluminescence method has been used as a on-site rapid detection method in nosocomial infections control more and more. In this study, a paired design between four methods/detectors were performed to detect the environmental surface after disinfection. Two methods were ATP bioluminescence method and colony counting method (C), and ATP bioluminescence method included three kinds of detectors (B, P and N). Every surface was performed by two methods/detectors. ATP content per surface from ICU had no statistically significant difference with internal medicine wards of B and P, of which p was 0.435 and 0.260. By Spearman rank correlation coefficients, with the exception of better correlation between ATP content detected by B and P, the correlation between the remaining methods/detectors was poor or had no correlation. And the differences between detectors are statistically significant. Therefor, ATP bioluminescence method may not be suitable for the evaluation of disinfection effect, but be more appropriate for evaluating the cleanliness of environmental surface.

Evaluation of a modified Karnofsky score to assess physical and psychological wellbeing of cats in a hospital setting

2016 ◽  
Vol 18 (11) ◽  
pp. 913-920 ◽  
Author(s):  
Elien RL Taffin ◽  
Dominique Paepe ◽  
Miguel Campos ◽  
Luc Duchateau ◽  
Nesya Goris ◽  
...  
Keyword(s):  
Hospital Setting ◽  
Rank Correlation ◽  
Reliability And Validity ◽  
Correlation Coefficients ◽  
Absolute Difference ◽  
Karnofsky Score ◽  
Health And Wellbeing ◽  
Significant Difference ◽  
Immunodeficiency Virus ◽  
High Degree

Objectives The Karnofsky score (KS) modified for cats, a scoring system to rate health and quality of life (QOL) in cats, is used in clinical trials, but its reliability and validity are yet to be determined. The present study aims to evaluate the scientific robustness of the KS when adapted for use in a hospital setting. Methods A list of variables to consider during the physical examination, which informs the clinician’s score (CS) part of the KS, was added and clinicians were allowed to choose a score anywhere between 0 and 50. The Karnofsky QOL questionnaire was adapted for use in a hospital setting. F-tests with Bonferroni correction and Spearman rank correlation coefficients were used to evaluate reliability and validity of the KS to assess the health and wellbeing of cats in a hospital setting. The records of 54 feline immunodeficiency virus-positive cats, which were recruited for a clinical trial and hospitalised for 6 weeks, were reviewed. Four veterinarians scored the CS, and one veterinarian and a veterinary nurse assessed the QOL score. Results Mean absolute difference between observers was significantly larger for the CS than for the QOL score ( P <0.001) and two veterinarians scored significantly higher than the remaining two veterinarians ( P <0.001). Inter-observer correlation ranged from 0.45–0.75 for the CS. For the QOL score, the absolute difference between observers was small, no significant difference was found between observers and a high degree of inter-observer correlation was noted (r = 0.91). Conclusions and relevance The results indicate low inter-observer reliability for the CS, requiring additional modifications to this part of the KS. The QOL score seems more reliable, and the questionnaire may serve as a reliable tool in the assessment of QOL in cats in a hospital setting. Consequently, further adaptation of the KS is mandatory when simultaneous assessment of both the cat’s clinical health and perceived wellbeing is required.

scholarly journals Assessment of temperament and its effect on weaning performance for Aubrac and Charolais weaned calves

2009 ◽  
pp. 161-169
Author(s):  
Andrea Szentléleki ◽  
Zoltán Domokos ◽  
Claudio Blottura ◽  
Massimiliano Alberti ◽  
János Tőzsér
Keyword(s):  
Rank Correlation ◽  
Correlation Coefficients ◽  
Spearman Rank Correlation ◽  
Point Scale ◽  
Bull Calves ◽  
Significant Difference ◽  
Weaning Age ◽  
Scale Test ◽  
Spearman Rank Correlation Test ◽  
Calf Rearing

The purposes of the present study are to compare the temperament of calves of different breed and sex at weaning, to analyse the effect of temperament on weaning performance, and todefine the correlations  between the previous traits. In the experiment carried out in Gelej in 2006 the behaviour of 61 Aubrac and 25 Charolais calves (39 heifer and 47 bull calves) wasassessed at weaning. The weight (WW) and the age of animals were detected, in addition temperament (TEMP) of them was scored. The weaning age (AU: 190±16.96 days, CH: 176±14.94days; P<0.001) and the weaning weight adjusted to 205 days of age (CWW) (AU: 192.39±31.32 kg, CH: 213.80±23.99 kg; P<0.01) of the two breeds significantly differed of each other. Thetemperament of calves was evaluated by scale test on a 5-point scale during weighing. The data were processed by SPSS 14.0 program package (Mann-Whitney U test, MANOVA, Tukey test,Spearman rank correlation test). Significant difference was revealed between the two breeds in TEMP (AU: 2 scores and CH: 3 scores; P<0.001), furthermore also difference was provenbetween the bull calves of Aubrac and Charolais in the behaviour (AU: 2 scores and CH: 3 scores, P<0.01). Analysing the effect of sex both for 86 calves and each breed, it was showed that the TEMP of the heifer and bull calves was equalled (P>0.10). Both of the WW and CWW were influenced by the behaviour of calves (P<0.05), which meant that the more excitable calves had higher WW and CWW. Positive, weak correlation coefficients were calculated among the TEMP, the WW and the CWW (rrank=0.28 and rrank=0.31; P<0.01). By their results it was concluded that in young age not only the performance but also the behaviour of calves are determined by the calf rearing ability of suckling cow. 

scholarly journals Reduced Sampling Frequency for Evaluating Fungicide Efficacy on Botrytis Fruit Rot of Strawberry

Plant Disease ◽  
2000 ◽  
Vol 84 (7) ◽  
pp. 743-748 ◽  
Author(s):  
D. E. Legard ◽  
F. G. Martin ◽  
C. L. Xiao ◽  
C. K. Chandler
Keyword(s):  
Production Systems ◽  
Rank Correlation ◽  
Correlation Coefficients ◽  
Complete Data ◽  
Fruit Rot ◽  
Data Sets ◽  
Marketable Yield ◽  
Data Set ◽  
Significant Difference ◽  
Fungicide Efficacy

Evaluating fungicide efficacy in annual strawberry production systems can be labor intensive due to continuous harvesting over a relatively long season. The effect of reduced harvest number on the accuracy of least significant difference (LSD) separations for Botrytis fruit rot (Botrytis cinerea) incidence and marketable yield in fungicide efficacy studies was evaluated over three seasons. Fruit were harvested and graded twice a week for a total of 23 to 32 harvests each season. Data from each season were divided into different sample sets. Data from three different harvest periods (early, late, and whole season) and different harvesting frequencies (twice weekly, once weekly, every second, third, or fourth week) were compared with the complete data set (twice weekly for the whole season). Spearman's rank correlation and Pearson's product moment correlation coefficients were used to evaluate the correlation of the complete data sets with data sets from other sampling plans. Harvesting once a week for either the late- or whole-season periods accurately estimated LSD groupings for Botrytis fruit rot incidence among fungicide treatments. The precision of marketable yield estimates using once-a-week harvesting for the late or whole-season periods were relatively lower than for the incidence of Botrytis.

scholarly journals Evaluation of different detector types in measurement of ATP bioluminescence compared to colony counting method for measuring bacterial burden of hospital surfaces

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0221665
Author(s):  
Huiqiong Xu ◽  
Jiansheng Liang ◽  
Yimei Wang ◽  
Bin Wang ◽  
Tianbao Zhang ◽  
...  
Keyword(s):  
Bacterial Burden ◽  
Counting Method ◽  
Colony Counting ◽  
Atp Bioluminescence

APPLICATION OF THE ATP-BIOLUMINESCENCE METHOD TO ASSESS THE QUALITY OF SANITATION OF MILKING EQUIPMENT

2020 ◽  
Vol 1 (4) ◽  
pp. 468-472
Author(s):  
P.A. Popov ◽  
◽  
D.V. Gruznov ◽  
S.V. Tokarev ◽  
◽  
...  
Keyword(s):  
Dairy Farms ◽  
Laboratory Studies ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Microbial Number ◽  
Proportional Relationship ◽  
The Relationship ◽  
Number Of Bacteria ◽  
Bioluminescence Method

The article presents the results of determining the relationship between the total microbial number and microbial ATP on the surface of milking equipment before and after treatment with neutral anolyte ANK-SUPER. The possibility of using the ATP-bioluminescence method to control the quality of sanitation of milking equipment on dairy farms is shown. Laboratory studies revealed a proportional relationship between the level of bacterial ATP and the number of bacteria on the surface of milking equipment before and after sanitation.

scholarly journals A Preliminary Study on Dependence of Mercury Distribution on the Degree of Coalification in Ningwu Coalfield, Shanxi, China

Energies ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3119
Author(s):  
Yinjiao Su ◽  
Xuan Liu ◽  
Yang Teng ◽  
Kai Zhang
Keyword(s):  
Linear Correlation ◽  
Bituminous Coal ◽  
Rank Correlation ◽  
Correlation Coefficients ◽  
Chemical Extraction ◽  
Mercury Distribution ◽  
Sequential Chemical Extraction ◽  
Average Proportion ◽  
Total Proportion ◽  
The Relationship

Mercury (Hg) is a toxic trace element emitted from coal conversion and utilization. Samples with different coal ranks and gangue from Ningwu Coalfield are selected and investigated in this study. For understanding dependence of mercury distribution characteristics on coalification degree, Pearson regression analysis coupled with Spearman rank correlation is employed to explore the relationship between mercury and sulfur, mercury and ash in coal, and sequential chemical extraction method is adopted to recognize the Hg speciation in the samples of coal and gangue. The measured results show that Hg is positively related to total sulfur content in coal and the affinity of Hg to different sulfur forms varies with the coalification degree. Organic sulfur has the biggest impact on Hg in peat, which becomes weak with increasing the coalification degree from lignite to bituminous coal. Sulfate sulfur is only related to Hg in peat or lignite as little content in coal. However, the Pearson linear correlation coefficients of Hg and pyritic sulfur are relatively high with 0.479 for lignite, 0.709 for sub-bituminous coal and 0.887 for bituminous coal. Hg is also related to ash content in coal, whose Pearson linear correlation coefficients are 0.504, 0.774 and 0.827 respectively, in lignite, sub-bituminous coal and bituminous coal. Furthermore, Hg distribution is directly depended on own speciation in coal. The total proportion of F2 + F3 + F4 is increased from 41.5% in peat to 87.4% in bituminous coal, but the average proportion of F5 is decreased from 56.8% in peat to 12.4% in bituminous coal. The above findings imply that both Hg and sulfur enrich in coal largely due to the migration from organic state to inorganic state with the increase of coalification degree in Ningwu Coalfield.

scholarly journals Curcumin-loaded polyvinyl butyral film with antibacterial activity

e-Polymers ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 673-681
Author(s):  
Yanchao Qiao ◽  
Lijie Duan
Keyword(s):  
Mechanical Property ◽  
Thermal Stability ◽  
Antibacterial Activity ◽  
Polyvinyl Butyral ◽  
Counting Method ◽  
E Coli ◽  
Antibacterial Materials ◽  
Good Antibacterial Activity ◽  
Colony Counting ◽  
Widespread Interest

AbstractAntibacterial materials have found widespread interest in different fields nowadays. In this study, curcumin (Cur) was incorporated into the polyvinyl butyral (PVB) matrix by dissolving in ethanol for improving the functional properties of a pure PVB film. We found that Cur was uniformly dispersed in the PVB matrix, which showed good compatibility. Moreover, the incorporation of Cur could also improve thermal stability, hydrophilicity, and mechanical property. The UV-vis spectra of the PVB–Cur film demonstrated that the film could block ultraviolet radiation. Subsequently, the antibacterial activity of the PVB–Cur film was measured by the colony-counting method against S. aureus and E. coli. The results showed that the PVB–Cur film exhibited good antibacterial activity. Therefore, the PVB–Cur film was considered as a promising material for food and medical packaging applications.

A prospective randomized study to assess the efficacy of post-operative nasal medication after endonasal surgery

1995 ◽  
Vol 109 (7) ◽  
pp. 618-621 ◽  
Author(s):  
Paul D. R. Spraggs ◽  
Marcelle Macnamara ◽  
Theo Joseph
Keyword(s):  
Randomized Study ◽  
Correlation Coefficients ◽  
Control Group ◽  
Time Intervals ◽  
Treatment Groups ◽  
Ephedrine Hydrochloride ◽  
Patent Airway ◽  
Significant Difference ◽  
Blood Clots ◽  
Betamethasone Sodium Phosphate

AbstractPost-operative nasal medications are commonly used following routine septal or turbinate surgery but their efficacy in removing blood clots, improving the sensation of a patent airway and promoting healing are unknown. This prospective randomized trial of patients undergoing septal and/or turbinate surgery assessed the efficacy of three commonly used nasal medicines, 0.5 per cent ephedrine hydrochloride nasal drops, betamethasone sodium phosphate (Betnosol®) nose drops and alkaline nasal douches, in producing the sensation of a patent airway in the 14 days following surgery. Ninety-seven patients were randomized into the three treatment groups and a control group who received no nasal medication. Patients assessed their nasal patency by means of a visual analogue scale (VAS) and any complications of treatment were recorded. Statistical analysis of the 76 complete sets of results using the Mann-Whitney U-test showed that there was a significant difference in the distribution of all of the treatments for each of the time intervals (p<0.05). Glass rank biserial correlation coefficients were all small (rg<0.085) but the most significant differences were between ephedrine and the control group at two hours, two, seven and 10 days (0.02, 0.054, 0.057, 0.085 respectively), alkaline nasal douches being most significant at four and 14 days (0.06 and 0.0722 respectively).

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