Frequency-dependent interference by magnetic fields of nerve growth factor-induced neurite outgrowth in PC-12 cells

1995 ◽  
Vol 16 (6) ◽  
pp. 387-395 ◽  
Author(s):  
C. F. Blackman ◽  
S. G. Benane ◽  
D. E. House
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Magnetic Fields ◽  
Neurite Outgrowth ◽  
Frequency Dependent ◽  
Nerve Growth ◽  
Pc 12 Cells

Nerve growth factor synthesis by intestinal epithelial cells

1995 ◽  
Vol 269 (3) ◽  
pp. G445-G452 ◽  
Author(s):  
G. W. Varilek ◽  
G. A. Neil ◽  
W. P. Bishop ◽  
J. Lin ◽  
N. J. Pantazis
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Neurite Outgrowth ◽  
Intestinal Epithelial Cells ◽  
Conditioned Media ◽  
Intestinal Epithelial ◽  
Nerve Growth ◽  
Pc 12 Cells ◽  
Isolated Rat

Nerve growth factor (NGF) exists in the gut of adult rats. The cells responsible for NGF synthesis in the gut remain unknown. IEC-6 and Caco-2 cells, established cell culture models of intestinal epithelial cells, were studied to determine whether intestinal epithelial cells, were studied to determine whether they synthesize and release NGF. Conditioned media from both IEC-6 and Caco-2 cells stimulated neurite outgrowth in both rat pheochromocytoma (PC-12) cells and sensory neurons derived from embryonic chick dorsal root ganglia (DRG). The addition of anti-NGF antibody blocked neurite outgrowth in PC-12 cells and partially blocked outgrowth in DRG cells. An NGF-enzyme-linked immunosorbant assay readily detected immunoreactive NGF in conditioned media from both cell lines, whereas cellular extracts from IEC-6, Caco-2, and isolated rat intestinal epithelial cells had low levels of immunoreactivity. Caco-2 monolayers primarily secreted NGF from the basolateral compartment, and interleukin-1 enhanced its secretion. IEC-6, Caco-2, and isolated rat intestinal epithelial cells expressed NGF mRNA as determined by reverse transcription polymerase chain reaction. These observations suggest that intestinal epithelial cells are capable of NGF synthesis.

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