Fluorescence depolarization studies of red cell membrane fluidity. The effect of exposure to 1.0-GHz microwave radiation

1981 ◽  
Vol 2 (1) ◽  
pp. 13-22 ◽  
Author(s):  
John W. Allis ◽  
Barbara L. Sinha
1995 ◽  
Vol 324 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Michael Hofmann ◽  
Peter Mainka ◽  
Hans Tritschler ◽  
Jürgen Fuchs ◽  
Guido Zimmer

1990 ◽  
Vol 190 (1-2) ◽  
pp. 57-66 ◽  
Author(s):  
Evelyne Peuchant ◽  
Claude Motta ◽  
Christine Salles ◽  
Michel Clerc

1995 ◽  
Vol 115 ◽  
pp. S108
Author(s):  
S.I. Muzulu ◽  
R.I. Norman ◽  
R.F. Bing

Blood ◽  
1984 ◽  
Vol 64 (5) ◽  
pp. 1129-1134 ◽  
Author(s):  
Y Yawata ◽  
T Sugihara ◽  
M Mori ◽  
S Nakashima ◽  
Y Nozawa

Abstract Membrane lipid analyses and electron spin resonance (ESR) studies of membrane fluidity were carried out on the red cells of a Japanese patient with hereditary high red cell membrane phosphatidylcholine hemolytic anemia (HPCHA). Increased amounts of phosphatidylcholine (PC) and cholesterol were found in the membrane lipids of the affected patient, despite normal plasma lipids. The order parameter of cholesterol-free pure phospholipid liposomes prepared from this patient's red cells was decreased, apparently because of the increased PC. In contrast, the order parameter of the total red cell lipid liposomes (containing free cholesterol) was essentially normal. The overall fluidity of the intact red cells was determined by ESR with a spin probe, 5-SAL. Again, the order parameters were normal in the intact red cells of the patient with HPCHA. This suggests that the concomitant increase of membrane cholesterol and phosphatidylcholine serves to maintain normal membrane fluidity in the HPCHA red cells.


Blood ◽  
1984 ◽  
Vol 64 (5) ◽  
pp. 1129-1134
Author(s):  
Y Yawata ◽  
T Sugihara ◽  
M Mori ◽  
S Nakashima ◽  
Y Nozawa

Membrane lipid analyses and electron spin resonance (ESR) studies of membrane fluidity were carried out on the red cells of a Japanese patient with hereditary high red cell membrane phosphatidylcholine hemolytic anemia (HPCHA). Increased amounts of phosphatidylcholine (PC) and cholesterol were found in the membrane lipids of the affected patient, despite normal plasma lipids. The order parameter of cholesterol-free pure phospholipid liposomes prepared from this patient's red cells was decreased, apparently because of the increased PC. In contrast, the order parameter of the total red cell lipid liposomes (containing free cholesterol) was essentially normal. The overall fluidity of the intact red cells was determined by ESR with a spin probe, 5-SAL. Again, the order parameters were normal in the intact red cells of the patient with HPCHA. This suggests that the concomitant increase of membrane cholesterol and phosphatidylcholine serves to maintain normal membrane fluidity in the HPCHA red cells.


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