Application of a temperature-dependent fluorescent dye (Rhodamine B) to the measurement of radiofrequency radiation-induced temperature changes in biological samples

2009 ◽  
Vol 30 (7) ◽  
pp. 583-590 ◽  
Author(s):  
Yuen Y. Chen ◽  
Andrew W. Wood
Keyword(s):  
Rhodamine B ◽  
Biological Samples ◽  
Fluorescent Dye ◽  
Temperature Dependent ◽  
Temperature Changes ◽  
Radiofrequency Radiation ◽  
Radiation Induced

Temperature Measurement in Microfluidic Systems Using Photobleaching of a Fluorescent Slab

2008 ◽  
Author(s):  
Lin Gui ◽  
Carolyn L. Ren
Keyword(s):  
Temperature Distribution ◽  
Temperature Measurement ◽  
Rhodamine B ◽  
Fluorescent Dye ◽  
Measured Temperature ◽  
Pdms Layer ◽  
Temperature Dependent ◽  
Buffer Solution ◽  
Fluorescent Intensity ◽  
Dye Diffusion

Temperature control is key to microfluidic-based Lab-on-a-Chip devices for a variety of applications such as polymerase chain reaction for DNA amplification and isoelectric focusing for protein separation where pH gradients are thermally generated. The most widely used temperature measurement method involves the mixing of the buffer solution with a fluorescent dye, which has a temperature-dependent fluorescent intensity. The temperature distribution in the liquid can be obtained by monitoring the fluorescent intensity distribution in the channel. However, this method can not be easily applied to polymer-made microfluidic chips because of dye absorption and penetration into polymer chips, electrophoresis of dye which causes artificial temperature gradients, and inevitable photobleaching of fluorescent dye. Therefore, a novel method is developed and presented here for temperature measurement by utilizing photobleaching of fluorescent dye. This method includes two novel contributions: i) a specially developed model for converting temperature-dependent photobleaching speed distribution to temperature distribution, and ii) an introduction of a thin polydimethylsiloxane (PDMS) layer with saturated Rhodamine B for solving the above-mentioned dye diffusion and electrophoresis problems. In this new method, a thin PDMS layer saturated with Rhodamine B is bonded with another PDMS layer with microchannels instead of mixing the dye with the buffer solution. Therefore, the problems associated with dye diffusion into PDMS chips and electrophoresis when an electrical field is applied to channels are avoided. The developed theory is validated by comparing the experimentally measured temperature distribution with numerical predicted results. The theory and its validation will be presented and discussed.

scholarly journals Microdosimetry Using Rhodamine B Within Macro- and Microsystems for Radiofrequency Signals Exposures of Biological Samples

2020 ◽  
Vol 68 (3) ◽  
pp. 1142-1150 ◽  
Author(s):  
Amani Nefzi ◽  
Lynn Carr ◽  
Claire Dalmay ◽  
Arnaud Pothier ◽  
Philippe Leveque ◽  
...  
Keyword(s):  
Rhodamine B ◽  
Biological Samples

Temperature-Dependent Photoluminescence Spectra of PbSe Quantum Dots for Temperature Markers

2012 ◽  
Vol 482-484 ◽  
pp. 2547-2550
Author(s):  
Peng Fei Gu ◽  
Ya Nan Wang ◽  
Jia Jia Cao ◽  
Yu Yan ◽  
Tie Qiang Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Temperature Sensitivity ◽  
Peak Position ◽  
Strong Temperature Dependence ◽  
Photoluminescence Spectra ◽  
Temperature Dependent ◽  
Temperature Variations ◽  
Temperature Changes ◽  
Optical Readout ◽  
Temperature Dependent Photoluminescence

We here report the temperature effect on photoluminescence(PL) spectra of PbSe quantum dots (QDs), which exhibit a strong temperature dependence on their spectra position and intensity. They potentially act as the temperature marker, sensing temperature variations and reporting temperature changes remotely through optical readout. In addition, the temperature sensitivity characterized by peak position of PbSe QDs was found to be 0.39nm/°C in a range of 10-100 °C.

scholarly journals Emission Characteristics of Fluorescent Labels with Respect to Temperature Changes and Subsequent Effects on DNA Microchip Studies

2005 ◽  
Vol 71 (10) ◽  
pp. 6453-6457 ◽  
Author(s):  
Wen-Tso Liu ◽  
Jer-Horng Wu ◽  
Emily Sze-Ying Li ◽  
Ezrein Shah Selamat
Keyword(s):  
Melting Curve ◽  
Melting Curve Analysis ◽  
Emission Characteristics ◽  
Fluorescent Labels ◽  
Temperature Dependent ◽  
Temperature Changes ◽  
Formamide Concentration ◽  
Effects Of Temperature ◽  
Microarray Studies ◽  
Texas Red

ABSTRACT The effects of temperature, salt concentration, and formamide concentration on the emission characteristics of commonly used fluorescent labels were evaluated on DNA microchips. The emission intensities of different fluorophores without hybridization were observed to vary, each to a different extent, to mainly temperature changes. Rhodamine red, TAMRA (tetramethylrhodamine), and dyes from the carbocyanide group exhibited the largest variations, and Texas Red and Oregon Green exhibited the smallest variations. This temperature dependency was shown to affect results obtained during melting curve analysis in DNA microarray studies. To minimize the bias associated with the temperature-dependent emission of different fluorescent labels, a normalization step was proposed.

The Temperature-Dependent Ideal Shear Strength of Solid Single Crystals

2018 ◽  
Vol 85 (3) ◽  
Author(s):  
Tianbao Cheng ◽  
Daining Fang ◽  
Yazheng Yang
Keyword(s):  
Shear Strength ◽  
Theoretical Model ◽  
Melting Point ◽  
Single Crystals ◽  
Elevated Temperatures ◽  
Absolute Zero ◽  
Temperature Dependent ◽  
Temperature Changes ◽  
First Time ◽  
The Ideal

Knowledge of the ideal shear strength of solid single crystals is of fundamental importance. However, it is very hard to determine this quantity at finite temperatures. In this work, a theoretical model for the temperature-dependent ideal shear strength of solid single crystals is established in the view of energy. To test the drawn model, the ideal shear properties of Al, Cu, and Ni single crystals are calculated and compared with that existing in the literature. The study shows that the ideal shear strength first remains approximately constant and then decreases almost linearly as temperature changes from absolute zero to melting point. As an example of application, the “brittleness parameter” of solids at elevated temperatures is quantitatively characterized for the first time.

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