Radiofrequency radiation-induced calcium ion efflux enhancement from human and other neuroblastoma cells in culture

1989 ◽  
Vol 10 (2) ◽  
pp. 197-202 ◽  
Author(s):  
S. K. Dutta ◽  
B. Ghosh ◽  
C. F. Blackman
Keyword(s):  
Calcium Ion ◽  
Neuroblastoma Cells ◽  
Radiofrequency Radiation ◽  
Cells In Culture ◽  
Ion Efflux ◽  
Radiation Induced

Effect of Amplitude-Modulated 147 MHz Radiofrequency Radiation on Calcium Ion Efflux from Avian Brain Tissue

1987 ◽  
Vol 109 (1) ◽  
pp. 19 ◽  
Author(s):  
Ernest N. Albert ◽  
Frank Slaby ◽  
Joseph Roche ◽  
John Loftus
Keyword(s):  
Brain Tissue ◽  
Calcium Ion ◽  
Avian Brain ◽  
Radiofrequency Radiation ◽  
Ion Efflux

Induction of calcium-ion efflux from brain tissue by radio-frequency radiation: Effects of modulation frequency and field strength

Radio Science ◽  
1979 ◽  
Vol 14 (6S) ◽  
pp. 93-98 ◽  
Author(s):  
C. F. Blackman ◽  
J. A. Elder ◽  
C. M. Weil ◽  
S. G. Benane ◽  
D. C. Eichinger ◽  
...  
Keyword(s):  
Field Strength ◽  
Radio Frequency ◽  
Brain Tissue ◽  
Calcium Ion ◽  
Radiation Effects ◽  
Modulation Frequency ◽  
Radio Frequency Radiation ◽  
Ion Efflux ◽  
Frequency Radiation

An Analysis of the Time-Dependent Changes in Intracellular Calcium Concentration in Endothelial Cells in Culture Induced by Mechanical Stimulation

1993 ◽  
Vol 115 (2) ◽  
pp. 160-168 ◽  
Author(s):  
F. K. Winston ◽  
L. E. Thibault ◽  
E. J. Macarak
Keyword(s):  
Endothelial Cells ◽  
Cell Membrane ◽  
Pulmonary Artery ◽  
Intracellular Calcium ◽  
Mechanical Stimulation ◽  
Calcium Ion ◽  
Time Course ◽  
Mechanical Strain ◽  
Structural Response ◽  
Cells In Culture

When bovine pulmonary artery endothelial cells in culture are subjected to mechanical strain, their physiology is altered. Experimentally, this mechanical strain is generated by increased tension in the substrate to which the cells are attached and results in altered levels of fibronectin. Studies of the structural response of the endothelial cell suggest that this stimulus is transmitted to the cell membrane, organelles, and cytoskeleton by natural cell attachments in a quantifiable and predictable manner. This report examines altered intracellular calcium homeostasis as a possible messenger for the observed strain-induced physiologic response. In particular, using the intracellularly trapped calcium indicator dyes, Quin2 and Fura2, we observed changes in cytosolic free calcium ion concentration in response to biaxial strain of bovine pulmonary artery endothelial cells in culture. The magnitude and time course of this calcium transient resemble that produced by treatment with the calcium ionophore, Ionomycin, indicating that mechanical stimulation may alter cell membrane permeability to calcium. Additional experiments in the presence of EDTA indicated that calcium was also released from intracellular stores in response to strain. In order to explain the stretch-induced calcium transients, a first-order species conservation model is presented that takes into account both the cell’s structural response and the calcium homeostatic mechanisms of the cell. It is hypothesized that the cell’s calcium sequestering and pumping capabilities balanced with its mechanically induced changes in calcium ion permeability will determine the level and time course of calcium accumulation in the cytosol.

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