Constitutive Secretory Expression and Characterization of Nitrilase from Alcaligenes faecalis in Pichia pastoris for Production of R ‐mandelic Acid

2021 ◽  
Author(s):  
Xinhong Zhang ◽  
Chuyan Wang ◽  
Yang Ge ◽  
Qingnan Meng ◽  
Yi Zhang
Keyword(s):  
Pichia Pastoris ◽  
Mandelic Acid ◽  
Alcaligenes Faecalis ◽  
Secretory Expression

Secretory expression and characterization of a soluble laccase from the Ganoderma lucidum strain 7071-9 in Pichia pastoris

2011 ◽  
Vol 39 (4) ◽  
pp. 3807-3814 ◽  
Author(s):  
Jing Sun ◽  
Ri-He Peng ◽  
Ai-Sheng Xiong ◽  
Yongsheng Tian ◽  
Wei Zhao ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Ganoderma Lucidum ◽  
Secretory Expression

Secretory Expression and Characterization of Chinese Narcissus GNA-Like Lectin in Pichia pastoris

2017 ◽  
Vol 182 (4) ◽  
pp. 1433-1443 ◽  
Author(s):  
Xinyu Li ◽  
Pengpeng Zhang ◽  
Ning Zhang ◽  
Xiao Liang ◽  
Wenya Wang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Secretory Expression

High-Level Secretory Expression and Purification of Recombinant Human Interleukin 1 Beta in Pichia pastoris

2016 ◽  
Vol 23 (8) ◽  
pp. 763-769 ◽  
Author(s):  
Pengfei Li ◽  
Ganggang Yang ◽  
Xiaofang Geng ◽  
Jinbao Shi ◽  
Bin Li ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Expression And Purification ◽  
Secretory Expression ◽  
High Level

scholarly journals Characterization of symbiotic and associated bacteria from entomopathogenic nematode Heterorhabditis sp. (nematode: Heterorhabditidae) isolated from India

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Rashid Pervez ◽  
Showkat Ahmad Lone ◽  
Sasmita Pattnaik
Keyword(s):  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Alcaligenes Faecalis ◽  
Symbiotic Bacteria ◽  
Rrna Gene ◽  
Main Body ◽  
Associated Bacteria ◽  
Molecular Approaches

Abstract Background Entomopathogenic nematodes (EPNs) harboring symbiotic bacteria are one of the safest alternatives to the chemical insecticides for the control of various insect pests. Infective juveniles of EPNs locate a target insect, enter through the openings, and reach the hemocoel, where they release the symbiotic bacteria and the target gets killed by the virulence factors of the bacteria. Photorhabdus with Heterorhabditis spp. are well documented; little is known about the associated bacteria. Main body In this study, we explored the presence of symbiotic and associated bacteria from Heterorhabditis sp. (IISR-EPN 09) and characterized by phenotypic, biochemical, and molecular approaches. Six bacterial isolates, belonging to four different genera, were recovered and identified as follows: Photorhabdus luminescens, one each strain of Providencia vermicola, Pseudomonas entomophila, Alcaligenes aquatilis, and two strains of Alcaligenes faecalis based on the phenotypic, biochemical criteria and the sequencing of 16S rRNA gene. Conclusion P. luminescens is symbiotically associated with Heterorhabditis sp. (IISR-EPN 09), whereas P. vermicola, P. entomophila, A. aquatilis, and A. faecalis are the associated bacteria. Further studies are needed to determine the exact role of the bacterial associates with the Heterorhabditis sp.

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