High-level secretory expression, purification, and  characterization of an anti-human Her II monoclonal  antibody, trastuzumab, in the methylotrophic yeast &lt;i&gt;Pichia pastoris&lt;/i&gt;

Tatsuro Shibui; Keisuke Bando; Satoru Misawa

doi:10.4236/abb.2013.45084

High-level secretory expression, purification, and characterization of an anti-human Her II monoclonal antibody, trastuzumab, in the methylotrophic yeast <i>Pichia pastoris</i>

Advances in Bioscience and Biotechnology ◽

10.4236/abb.2013.45084 ◽

2013 ◽

Vol 04 (05) ◽

pp. 640-646 ◽

Cited By ~ 6

Author(s):

Tatsuro Shibui ◽

Keisuke Bando ◽

Satoru Misawa

Keyword(s):

Monoclonal Antibody ◽

Pichia Pastoris ◽

Methylotrophic Yeast ◽

Purification And Characterization ◽

Secretory Expression ◽

High Level

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High-level secretory expression, purification and characterization of Ailuropoda melanoleuca growth hormone in Pichia pastoris

Protein Expression and Purification ◽

10.1016/j.pep.2008.03.001 ◽

2008 ◽

Vol 60 (2) ◽

pp. 182-187 ◽

Cited By ~ 1

Author(s):

Qinggang Xu ◽

Xuechang Wu ◽

Rong Hou ◽

Mingjuan Liao ◽

Kim Hyok Il ◽

...

Keyword(s):

Growth Hormone ◽

Pichia Pastoris ◽

Ailuropoda Melanoleuca ◽

Purification And Characterization ◽

Secretory Expression ◽

High Level

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Efficient expression, purification and characterization of mouse salivary ?-amylase secreted from methylotrophic yeast,Pichia pastoris

Yeast ◽

10.1002/yea.714 ◽

2001 ◽

Vol 18 (7) ◽

pp. 643-655 ◽

Cited By ~ 19

Author(s):

Shinya Kato ◽

Matsujiro Ishibashi ◽

Daisuke Tatsuda ◽

Hiroko Tokunaga ◽

Masao Tokunaga

Keyword(s):

Pichia Pastoris ◽

Methylotrophic Yeast ◽

Purification And Characterization ◽

Salivary Amylase ◽

Yeast Pichia Pastoris ◽

Methylotrophic Yeast Pichia Pastoris ◽

Efficient Expression

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High level expression, purification, and characterization of the shrimp antimicrobial peptide, Ch-penaeidin, in Pichia pastoris

Protein Expression and Purification ◽

10.1016/j.pep.2004.09.006 ◽

2005 ◽

Vol 39 (2) ◽

pp. 144-151 ◽

Cited By ~ 65

Author(s):

Lei Li ◽

Jin-Xing Wang ◽

Xiao-Fan Zhao ◽

Cui-Jie Kang ◽

Ning Liu ◽

...

Keyword(s):

Pichia Pastoris ◽

Antimicrobial Peptide ◽

Purification And Characterization ◽

High Level Expression ◽

High Level

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High-level expression, purification, and characterization of recombinant human basic fibroblast growth factor in Pichia pastoris

Protein Expression and Purification ◽

10.1016/j.pep.2008.02.009 ◽

2008 ◽

Vol 59 (2) ◽

pp. 282-288 ◽

Cited By ~ 25

Author(s):

Xupeng Mu ◽

Ning Kong ◽

Weili Chen ◽

Ting Zhang ◽

Mohan Shen ◽

...

Keyword(s):

Growth Factor ◽

Pichia Pastoris ◽

Fibroblast Growth Factor ◽

Basic Fibroblast Growth Factor ◽

Fibroblast Growth ◽

Purification And Characterization ◽

High Level Expression ◽

High Level

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High-level expression of Staphylococcal Protein A in Pichia pastoris and purification and characterization of the recombinant protein

Protein Expression and Purification ◽

10.1016/j.pep.2013.06.005 ◽

2013 ◽

Vol 90 (2) ◽

pp. 178-185 ◽

Cited By ~ 13

Author(s):

Jing Hao ◽

Li Xu ◽

Hongde He ◽

Xiaojun Du ◽

Lingyun Jia

Keyword(s):

Pichia Pastoris ◽

Recombinant Protein ◽

Protein A ◽

Staphylococcal Protein A ◽

Purification And Characterization ◽

High Level Expression ◽

Staphylococcal Protein ◽

High Level

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Purification and Characterization of an Intracellular β-Glucosidase from the Methylotrophic Yeast Pichia pastoris

Biochemistry (Moscow) ◽

10.1007/s10541-005-0270-5 ◽

2005 ◽

Vol 70 (12) ◽

pp. 1363-1368 ◽

Cited By ~ 25

Author(s):

Y. Turan ◽

M. Zheng

Keyword(s):

Pichia Pastoris ◽

Methylotrophic Yeast ◽

Purification And Characterization ◽

Yeast Pichia Pastoris ◽

Methylotrophic Yeast Pichia Pastoris

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High-level expression, purification, and characterization of recombinant wheat xylanase inhibitor TAXI-I secreted by the yeast Pichia pastoris

Protein Expression and Purification ◽

10.1016/j.pep.2004.05.014 ◽

2004 ◽

Vol 37 (1) ◽

pp. 39-46 ◽

Cited By ~ 23

Author(s):

Katleen Fierens ◽

Nele Geudens ◽

Kristof Brijs ◽

Christophe M Courtin ◽

Kurt Gebruers ◽

...

Keyword(s):

Pichia Pastoris ◽

Purification And Characterization ◽

High Level Expression ◽

Yeast Pichia Pastoris ◽

Xylanase Inhibitor ◽

High Level

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High-level expression, purification and characterization of recombinant Aspergillus oryzae alkaline protease in Pichia pastoris

Protein Expression and Purification ◽

10.1016/j.pep.2007.12.005 ◽

2008 ◽

Vol 58 (2) ◽

pp. 301-308 ◽

Cited By ~ 29

Author(s):

Ji-Ping Guo ◽

Ying Ma

Keyword(s):

Pichia Pastoris ◽

Aspergillus Oryzae ◽

Alkaline Protease ◽

Purification And Characterization ◽

High Level Expression ◽

High Level

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High-level expression, purification, and enzymatic characterization of truncated poly(vinyl alcohol) dehydrogenase in methylotrophic yeast Pichia pastoris

Applied Microbiology and Biotechnology ◽

10.1007/s00253-012-3986-3 ◽

2012 ◽

Vol 97 (3) ◽

pp. 1113-1120 ◽

Cited By ~ 8

Author(s):

Dongxu Jia ◽

Jianghua Li ◽

Long Liu ◽

Dongxu Zhang ◽

Yu Yang ◽

...

Keyword(s):

Pichia Pastoris ◽

Alcohol Dehydrogenase ◽

Methylotrophic Yeast ◽

Poly Vinyl Alcohol ◽

Enzymatic Characterization ◽

High Level Expression ◽

Yeast Pichia Pastoris ◽

Methylotrophic Yeast Pichia Pastoris ◽

High Level

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High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris

Biochemistry ◽

10.1021/bi00435a074 ◽

1989 ◽

Vol 28 (9) ◽

pp. 4117-4125 ◽

Cited By ~ 104

Author(s):

K. Sreekrishna ◽

Lynn Nelles ◽

Rica Potenz ◽

John Cruze ◽

Paul Mazzaferro ◽

...

Keyword(s):

Tumor Necrosis ◽

Human Tumor ◽

Methylotrophic Yeast ◽

Human Tumor Necrosis Factor ◽

Purification And Characterization ◽

High Level Expression ◽

Methylotrophic Yeast Pichia Pastoris ◽

High Level ◽

Necrosis Factor

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