Immobilized peptide‐N‐glycosidase F onto magnetic nanoparticles: A biotechnological tool for protein deglycosylation under native conditions

2021 ◽  
Author(s):  
Lucía Bidondo ◽  
Florencia Festari ◽  
Teresa Freire ◽  
Cecilia Giacomini
Keyword(s):  
Magnetic Nanoparticles ◽  
Immobilized Peptide

Thermal Conductivity of Nanofluid with Magnetic Nanoparticles

PIERS Online ◽  
2009 ◽  
Vol 5 (3) ◽  
pp. 231-234 ◽  
Author(s):  
Tsung-Han Tsai ◽  
Long-Sheng Kuo ◽  
Ping-Hei Chen ◽  
Chin-Ting Yang
Keyword(s):  
Thermal Conductivity ◽  
Magnetic Nanoparticles

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

Magnetic Nanoparticles for Extracting DNA from Blood Cells

2020 ◽  
Vol 84 (11) ◽  
pp. 1362-1365
Author(s):  
A. V. Komina ◽  
R. N. Yaroslavtsev ◽  
Y. V. Gerasimova ◽  
S. V. Stolyar ◽  
I. A. Olkhovsky ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Blood Cells

MR Molecular Imaging Based on Magnetic Nanoparticles

2018 ◽  
pp. 17-28
Author(s):  
Hwunjae Lee ◽  
◽  
SangBock Lee ◽  
Geahwan Jin ◽  
Sergey NETESOV ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Molecular Imaging

MR Molecular Imaging Based on Magnetic Nanoparticles

2018 ◽  
pp. 17-28
Author(s):  
Hwunjae Lee ◽  
◽  
SangBock Lee ◽  
Geahwan Jin ◽  
Sergey NETESOV ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Molecular Imaging

INFLUENCE OF MAGNETIC NANOPARTICLES ON ACOUSTIC ATTENUATION IN LIQUIDS

Akustika ◽  
2020 ◽  
pp. 8-13
Author(s):  
Štefan Hardoň ◽  
Jozef Kúdelčík
Keyword(s):  
Magnetic Nanoparticles ◽  
Heat Exchangers ◽  
Magnetic Fluids ◽  
Magnetic Flux Density ◽  
Acoustic Attenuation ◽  
Cooling Systems ◽  
Practical Applications ◽  
Acoustic Spectroscopy ◽  
Aggregation Processes ◽  
Diagnostics And Therapy

Magnetic fluids with nanoparticles dispersed in water or oils offer attractive applications in biomedicine and industry. Biocompatible magnetic fluids are used for diagnostics and therapy in medical applications, in pharmacy, and biosensors. Application of ferrofluids is expanding into energy conservation, faster and efficient cooling, and hence better performance in a wide variety of practical applications (in heat exchangers, mainly in micro-cooling systems). For the study of the influence of an external magnetic field on the aggregation processes of magnetic nanoparticles in magnetic fluids, acoustic spectroscopy was used. The jump changes of the magnetic flux density at various temperatures influenced the acoustic attenuation. The measured changes were results of nanoparticle aggregations into new structures.

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