Genome mining and functional analysis of cytochrome P450 genes involved in insecticide resistance in Leucinodes orbonalis (Lepidoptera: Crambidae)

2020 ◽  
Author(s):  
Bheeranna Kariyanna ◽  
Aralimarad Prabhuraj ◽  
Ramasamy Asokan ◽  
Govindaraju Ramkumar ◽  
Thiruvengadam Venkatesan ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Cytochrome P450 ◽  
Insecticide Resistance ◽  
Genome Mining ◽  
Leucinodes Orbonalis ◽  
Cytochrome P450 Genes

scholarly journals Genome Mining and Expression Analysis of Carboxylesterase and Glutathione S-Transferase Genes Involved in Insecticide Resistance in Eggplant Shoot and Fruit Borer, Leucinodes orbonalis (Lepidoptera: Crambidae)

2020 ◽  
Vol 11 ◽  
Author(s):  
B. Kariyanna ◽  
A. Prabhuraj ◽  
R. Asokan ◽  
A. Agrawal ◽  
R. Gandhi Gracy ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Management Strategies ◽  
Draft Genome ◽  
Genome Mining ◽  
Gene Families ◽  
Detoxification Enzymes ◽  
Whole Genome Sequence ◽  
Sequence Information ◽  
Leucinodes Orbonalis ◽  
Fruit Borer

The shoot and fruit borer, Leucinodes orbonalis (Lepidoptera: Crambidae) is the major cause of low productivity in eggplant and insecticides being the mainstay of management of L. orbonalis. However, field control failures are widespread due to the evolution of insecticide resistance. Taking advantage of the whole genome sequence information, the present study investigated the level of insecticide resistance and the expression pattern of individual carboxylesterase (CE) and glutathione S-transferases (GSTs) genes in various field collected populations of L. orbonalis. Dose-mortality bioassays revealed a very high level of resistance development against fenvalerate (48.2–160-fold), phosalone (94-534.6-fold), emamectin benzoate (7.2–55-fold), thiodicarb (9.64–22.7-fold), flubendiamide (187.4–303.0-fold), and chlorantraniliprole (1.6–8.6-fold) in field populations as compared to laboratory-reared susceptible iso-female colony (Lo-S). Over-production of detoxification enzymes viz., CE and GST were evident upon enzyme assays. Mining of the draft genome of L. orbonalis yielded large number of genes potentially belonging to the CE and GST gene families with known history of insecticide resistance in other insects. Subsequent RT-qPCR studies on relative contribution of individual genes revealed over-expression of numerous GSTs and few CEs in field populations, indicating their possible involvement of metabolic enzymes in insecticide resistance. The genomic information will facilitate the development of novel resistance management strategies against this pest.

Expression and functional analysis of cytochrome P450 genes in the integument of the oriental armyworm, Mythimna separata ( Walker )

2020 ◽  
Vol 77 (1) ◽  
pp. 577-587
Author(s):  
Yong Huang ◽  
Hongqin Yin ◽  
Zeng Zhu ◽  
Xingchuan Jiang ◽  
Xiuxia Li ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Cytochrome P450 ◽  
Mythimna Separata ◽  
Cytochrome P450 Genes ◽  
Oriental Armyworm

Evaluating the insecticide resistance potential of eight Drosophila melanogaster cytochrome P450 genes by transgenic over-expression

2007 ◽  
Vol 37 (5) ◽  
pp. 512-519 ◽  
Author(s):  
Phillip J. Daborn ◽  
Christopher Lumb ◽  
Adrian Boey ◽  
Wayn Wong ◽  
Richard H. ffrench-Constant ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Cytochrome P450 ◽  
Insecticide Resistance ◽  
Over Expression ◽  
Cytochrome P450 Genes

scholarly journals Cytochrome P450 monooxygenases and insecticide resistance in insects

1998 ◽  
Vol 353 (1376) ◽  
pp. 1701-1705 ◽  
Author(s):  
J. Bergé ◽  
R. Feyereisen ◽  
M. Amichot
Keyword(s):  
Cytochrome P450 ◽  
Insecticide Resistance ◽  
3D Structure ◽  
Resistance Mechanisms ◽  
Cytochrome P450 Monooxygenases ◽  
Wild Type ◽  
P450 Gene ◽  
P450 Monooxygenases ◽  
Loss Of Resistance ◽  
Cytochrome P450 Genes

Cytochrome P450 monooxygenases are involved in many cases of resistance of insects to insecticides. Resistance has long been associated with an increase in monooxygenase activities and with an increase in cytochrome P450 content. However, this increase does not always account for all of the resistance. In Drosophila melanogaster , we have shown that the overproduction of cytochrome P450 can be lost by the fly without a corresponding complete loss of resistance. These results prompted the sequencing of a cytochrome P450 candidate for resistance in resistant and susceptible flies. Several mutations leading to amino–acid substitutions have been detected in the P450 gene CYP6A2 of a resistant strain. The location of these mutations in a model of the 3D structure of the CYP6A2 protein suggested that some of them may be important for enzyme activity of this molecule. This has been verified by heterologous expression of wild–type and mutated cDNA in Escherichia coli . When other resistance mechanisms are considered, relatively few genetic mutations are involved in insecticide resistance, and this has led to an optimistic view of the management of resistance. Our observations compel us to survey in more detail the genetic diversity of cytochrome P450 genes and alleles involved in resistance.

Functional analysis of cytochrome P450 genes linked with acetamiprid resistance in melon aphid, Aphis gossypii

2020 ◽  
Vol 170 ◽  
pp. 104687 ◽  
Author(s):  
Farman Ullah ◽  
Hina Gul ◽  
Kaleem Tariq ◽  
Nicolas Desneux ◽  
Xiwu Gao ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Cytochrome P450 ◽  
Aphis Gossypii ◽  
Cytochrome P450 Genes ◽  
Melon Aphid

scholarly journals Escalation of Pyrethroid Resistance in the Malaria Vector Anopheles funestus Induces a Loss of Efficacy of Piperonyl Butoxide–Based Insecticide-Treated Nets in Mozambique

2019 ◽  
Vol 220 (3) ◽  
pp. 467-475 ◽  
Author(s):  
Jacob M Riveron ◽  
Silvie Huijben ◽  
Williams Tchapga ◽  
Magellan Tchouakui ◽  
Murielle J Wondji ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Pyrethroid Resistance ◽  
Anopheles Funestus ◽  
Resistance Mechanisms ◽  
Piperonyl Butoxide ◽  
Insecticide Treated Nets ◽  
Molecular Analyses ◽  
Cytochrome P450 Genes ◽  
High Degree ◽  
Long Lasting Insecticidal Nets

Abstract Background Insecticide resistance poses a serious threat to insecticide-based interventions in Africa. There is a fear that resistance escalation could jeopardize malaria control efforts. Monitoring of cases of aggravation of resistance intensity and its impact on the efficacy of control tools is crucial to predict consequences of resistance. Methods The resistance levels of an Anopheles funestus population from Palmeira, southern Mozambique, were characterized and their impact on the efficacy of various insecticide-treated nets established. Results A dramatic loss of efficacy of all long-lasting insecticidal nets (LLINs), including piperonyl butoxide (PBO)–based nets (Olyset Plus), was observed. This An. funestus population consistently (2016, 2017, and 2018) exhibited a high degree of pyrethroid resistance. Molecular analyses revealed that this resistance escalation was associated with a massive overexpression of the duplicated cytochrome P450 genes CYP6P9a and CYP6P9b, and also the fixation of the resistance CYP6P9a_R allele in this population in 2016 (100%) in contrast to 2002 (5%). However, the low recovery of susceptibility after PBO synergist assay suggests that other resistance mechanisms could be involved. Conclusions The loss of efficacy of pyrethroid-based LLINs with and without PBO is a concern for the effectiveness of insecticide-based interventions, and action should be taken to prevent the spread of such super-resistance.

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