Antioxidant and reactive oxygen species scavenging properties of cellular albumin in HepG2 cells is mediated by the glutathione redox system

2018 ◽  
Vol 66 (2) ◽  
pp. 163-171 ◽  
Author(s):  
Ali Seidkhani‐Nahal ◽  
Abdolamir Allameh ◽  
Masoud Soleimani
Keyword(s):  
Reactive Oxygen Species ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Redox System ◽  
Oxygen Species ◽  
Glutathione Redox System ◽  
Glutathione Redox

scholarly journals Yap1-Regulated Glutathione Redox System Curtails Accumulation of Formaldehyde and Reactive Oxygen Species in Methanol Metabolism of Pichia pastoris

2009 ◽  
Vol 8 (4) ◽  
pp. 540-549 ◽  
Author(s):  
Taisuke Yano ◽  
Emiko Takigami ◽  
Hiroya Yurimoto ◽  
Yasuyoshi Sakai
Keyword(s):  
Reactive Oxygen Species ◽  
Pichia Pastoris ◽  
Critical Role ◽  
Reactive Oxygen ◽  
Redox System ◽  
Reduced Form ◽  
Growth Defect ◽  
Oxygen Species ◽  
Glutathione Redox System ◽  
Glutathione Redox

ABSTRACT The glutathione redox system, including the glutathione biosynthesis and glutathione regeneration reaction, has been found to play a critical role in the yeast Pichia pastoris during growth on methanol, and this regulation was at least partly executed by the transcription factor PpYap1. During adaptation to methanol medium, PpYap1 transiently localized to the nucleus and activated the expression of the glutathione redox system and upregulated glutathione reductase 1 (Glr1). Glr1 activates the regeneration of the reduced form of glutathione (GSH). Depletion of Glr1 caused a severe growth defect on methanol and hypersensitivity to formaldehyde (HCHO), which could be complemented by addition of GSH to the medium. Disruption of the genes for the HCHO-oxidizing enzymes PpFld1 and PpFgh1 caused a comparable phenotype, but disruption of the downstream gene PpFDH1 did not, demonstrating the importance of maintaining intracellular GSH levels. Absence of the peroxisomal glutathione peroxidase Pmp20 also triggered nuclear localization of PpYap1, and although cells were not sensitive to HCHO, growth on methanol was again severely impaired due to oxidative stress. Thus, the PpYap1-regulated glutathione redox system has two important roles, i.e., HCHO metabolism and detoxification of reactive oxygen species.

scholarly journals Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-8
Author(s):  
Xiao-Tian Zhang ◽  
Chun-Jiang Yu ◽  
Jian-Wei Liu ◽  
Yan-Ping Zhang ◽  
Chao Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Palmitic Acid ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Glucose Consumption ◽  
Control Group ◽  
High Dose ◽  
Oxygen Species ◽  
Insulin Resistant

We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro. After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ. Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells. The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P< 0.001); however, QJJ restored HepG2 cell survival (P< 0.001). As compared with HepG2 cells in the IR group, QJJ at all doses analyzed significantly increased glucose consumption (allP< 0.05). Moreover, treatment with all the QJJ doses significantly reduced the mean intracellular reactive oxygen species levels as compared with the IR group (allP< 0.05). Furthermore, high-dose QJJ reduced both TNF-αand IL-6 levels as compared to the IR group (allP< 0.05). QJJ ameliorated the altered PI3K, GLUT4, and RAGE expression observed with IR. In conclusion, QJJ can improve IR in HepG2 cells, which may be mediated through the IRS-1/PI3K/GLUT4 signaling pathway as well as regulation of NF-κB-mediated inflammation and oxidative stress.

Reactive oxygen species-dependent JNK downregulated olaquindox-induced autophagy in HepG2 cells

2014 ◽  
Vol 35 (7) ◽  
pp. 709-716 ◽  
Author(s):  
Dongxu Zhao ◽  
Congcong Wang ◽  
Shusheng Tang ◽  
Chaoming Zhang ◽  
Shen Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

Neferine induces reactive oxygen species mediated intrinsic pathway of apoptosis in HepG2 cells

2013 ◽  
Vol 136 (2) ◽  
pp. 659-667 ◽  
Author(s):  
Paramasivan Poornima ◽  
Robin Sheeba Quency ◽  
Viswandha Vijaya Padma
Keyword(s):  
Reactive Oxygen Species ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Intrinsic Pathway
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