Immunoassays of fungal laccases for screening of natural enzymes and control of recombinant enzyme production

2014 ◽  
Vol 61 (2) ◽  
pp. 230-236 ◽  
Author(s):  
Dmitry S. Loginov ◽  
Ekaterina A. Vavilova ◽  
Оlga S. Savinova ◽  
Alfia R. Abyanova ◽  
Andrey M. Chulkin ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Recombinant Enzyme ◽  
Fungal Laccases ◽  
And Control

Economic and Environmental Evaluation of Recombinant Enzyme Production

2015 ◽  
pp. 129-142
Author(s):  
Mohd Jamil Aizat Jamaluddin ◽  
Azlin Suhaida Azmi ◽  
Sarina Sulaiman ◽  
Dzun Noraini Jimat ◽  
Muhd. Ezza Faiez Othman ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Recombinant Enzyme ◽  
Environmental Evaluation

Optimising enzyme production by bakers yeast in continuous culture: physiological knowledge useful for process design and control

1996 ◽  
Vol 15 (5) ◽  
pp. 239-245 ◽  
Author(s):  
M. Bulmer ◽  
I. D. L. Bogle ◽  
N. Titchener-Hooker ◽  
M. E. Gregory
Keyword(s):  
Continuous Culture ◽  
Process Design ◽  
Enzyme Production ◽  
And Control

Correlation between enzyme production, germ tube formation and susceptibility to fluconazole in Candida species isolated from patients with denture-related stomatitis and control individuals

2008 ◽  
Vol 37 (10) ◽  
pp. 587-592 ◽  
Author(s):  
Eugénia Pinto ◽  
Isabel Cristina Ribeiro ◽  
Núria Joana Ferreira ◽  
Cátia Eliana Fortes ◽  
Patrícia Alexandra Fonseca ◽  
...  
Keyword(s):  
Candida Species ◽  
Enzyme Production ◽  
Germ Tube ◽  
Tube Formation ◽  
And Control

scholarly journals The Cyclodextrin Glycosyltransferase ofPaenibacillus pabuliUS132 Strain: Molecular Characterization and Overproduction of the Recombinant Enzyme

2008 ◽  
Vol 2008 ◽  
pp. 1-9 ◽  
Author(s):  
Sonia Jemli ◽  
Ezzedine Ben Messaoud ◽  
Sameh Ben Mabrouk ◽  
Samir Bejar
Keyword(s):  
Enzyme Production ◽  
Shake Flask ◽  
Active Form ◽  
Culture Conditions ◽  
Recombinant Enzyme ◽  
Cyclodextrin Glycosyltransferase ◽  
Gene Encoding ◽  
High Enzyme ◽  
Shake Flasks ◽  
Mature Enzyme

The gene encoding the cyclodextrin glycosyltransferase (CGTase) ofPaenibacillus pabuliUS132, previously described as efficient antistaling agent and good candidate for cyclodextrins production, was cloned, sequenced, and expressed inEscherichia coli. Sequence analysis showed that the mature enzyme (684 amino acids) was preceded by a signal peptide of 34 residues. The enzyme exhibited the highest identity (94%) to theβ-CGTase ofBacillus circulansno. 8. The production of the recombinant CGTase, as active form, was very low (about 1 U/mL) in shake flasks at37∘C. This production reached 22 U/mL after 22 hours of induction by mainly shifting the postinduction temperature from 37 to19∘Cand using 2TY instead of LB medium. High enzyme production (35 U/mL) was attained after 18 hours of induction in fermentor using the same culture conditions as in shake flask. The recombinant enzyme showedVmax⁡andKmvalues of253±36 μmol ofβ-cyclodextrin/mg/min and0.36±0.18 g/L, respectively.

Optimization of Recombinant Enzyme Production with Pichia pastoris in an Integrated Industrial Scale-down Production Plant

2010 ◽  
Vol 43 (6) ◽  
pp. 293-298
Author(s):  
K. Loegering ◽  
C. Mueller ◽  
J. Fricke ◽  
H.-P. Bertelsen ◽  
U. Scheffler ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Enzyme Production ◽  
Recombinant Enzyme ◽  
Industrial Scale ◽  
Production Plant ◽  
Scale Down

An integrated scale-down plant for optimal recombinant enzyme production by Pichia pastoris

2011 ◽  
Vol 6 (4) ◽  
pp. 428-436 ◽  
Author(s):  
Kai Loegering ◽  
Christian Mueller ◽  
Jan-Patrick Voss ◽  
Claudia Wagenfuehrer ◽  
Daniel Zahn ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Enzyme Production ◽  
Recombinant Enzyme ◽  
Scale Down

Optimising enzyme production by bakers yeast in continuous culture: physiological knowledge useful for process design and control

1996 ◽  
Vol 15 (5) ◽  
pp. 239 ◽  
Author(s):  
M. E. Gregory ◽  
M. Bulmer ◽  
I. D. L. Bogle ◽  
N. Titchener-Hooker
Keyword(s):  
Continuous Culture ◽  
Process Design ◽  
Enzyme Production ◽  
And Control

Control of Metal Alloy Oxidation with Electric Fields

1973 ◽  
Vol 31 ◽  
pp. 164-165
Author(s):  
R. R. Dils ◽  
P. S. Follansbee
Keyword(s):  
Electric Fields ◽  
Oxidation Process ◽  
Base Alloy ◽  
Oxide Surface ◽  
Platinum Electrodes ◽  
Insulating Layer ◽  
Iron Base Alloy ◽  
Alloy Oxidation ◽  
Aluminum Oxide Layer ◽  
And Control

Electric fields have been applied across oxides growing on a high temperature alloy and control of the oxidation of the material has been demonstrated. At present, three-fold increases in the oxidation rate have been measured in accelerating fields and the oxidation process has been completely stopped in a retarding field.The experiments have been conducted with an iron-base alloy, Pe 25Cr 5A1 0.1Y, although, in principle, any alloy capable of forming an adherent aluminum oxide layer during oxidation can be used. A specimen is polished and oxidized to produce a thin, uniform insulating layer on one surface. Three platinum electrodes are sputtered on the oxide surface and the specimen is reoxidized.

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