A Dual Role of Upper Zone of Growth Plate and Cartilage Matrix–Associated Protein in Human and Mouse Osteoarthritic Cartilage: Inhibition of Aggrecanases and Promotion of Bone Turnover

2017 ◽  
Vol 69 (6) ◽  
pp. 1233-1245 ◽  
Author(s):  
Michael Stock ◽  
Stefanie Menges ◽  
Nicole Eitzinger ◽  
Maria Geßlein ◽  
Renate Botschner ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Growth Plate ◽  
Dual Role ◽  
Cartilage Matrix ◽  
Osteoarthritic Cartilage ◽  
Human And Mouse ◽  
And Cartilage

In vivo protective effects of upper zone of growth plate and cartilage matrix associated protein against cartilage degeneration in a monosodium iodoacetate induced osteoarthritis model

2020 ◽  
Vol 98 (11) ◽  
pp. 763-770
Author(s):  
Hamza Malik Okuyan ◽  
Menderes Yusuf Terzi ◽  
İhsan Karaboğa ◽  
Serdar Doğan ◽  
Aydıner Kalacı
Keyword(s):  
Growth Plate ◽  
Matrix Protein ◽  
Critical Role ◽  
Cartilage Degeneration ◽  
Cartilage Matrix ◽  
Bone Morphogenetic Protein 2 ◽  
Monosodium Iodoacetate ◽  
And Cartilage

Osteoarthritis (OA) is a degenerative disease affecting the majority of over 65 year old people and characterized by cartilage degeneration, subchondral abnormal changes, and inflammation. Despite the enormous socioeconomic burden caused by OA, currently, there is no effective therapy against it. Upper zone of growth plate and cartilage matrix associated protein (UCMA) is a vitamin K dependent protein and has a critical role in pathophysiological conditions associated with bone and cartilage. However, there is no research on the protective role of intra-articular UCMA treatment in OA pathogenesis. Therefore, we aimed to investigate the potential therapeutic role of UCMA in an in vivo model of OA. We report for the first time that intra-articular UCMA injection ameliorated cartilage degeneration in a monosodium iodoacetate induced OA rat model. Furthermore, the OA-induced activation of nuclear factor kappa B and bone morphogenetic protein 2 signals was attenuated by UCMA. Our results indicated that UCMA decreased cartilage oligomeric matrix protein levels but did not affect interleukin 6, total antioxidant status, and total oxidant status levels in the serum. In conclusion, UCMA exhibited a therapeutic potential in the treatment of OA. This protective effect of UCMA is possibly achieved by reducing the aggrecanase activity and the production of inflammatory cytokines.

scholarly journals Acupuncture Delays Cartilage Degeneration through Upregulating SIRT1 Expression in Rats with Osteoarthritis

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Hui Liu ◽  
Tingting Zhang ◽  
Min Liu ◽  
Chunhong Wang ◽  
Jinfeng Yan
Keyword(s):  
Cartilage Degeneration ◽  
Cartilage Matrix ◽  
Enzyme Linked Immunosorbent Assay ◽  
Matrix Degradation ◽  
Matrix Synthesis ◽  
Sirt1 Expression ◽  
Tnf Α ◽  
Related Proteins ◽  
And Cartilage

Silent mating type information regulation 2 homolog 1 (SIRT1) has been reported to inhibit osteoarthritic gene expression in chondrocytes. Here, efforts in this study were made to unveil the specific role of SIRT1 in the therapy of acupuncture on cartilage degeneration in osteoarthritis (OA). Specifically, OA was established by the anterior cruciate ligament transection method in the right knee joint of rats, subsequent to which acupuncture was performed on two acupoints. Injection with shSIRT1 sequence–inserted lentiviruses was conducted to investigate the role of SIRT1 in acupuncture-mediated OA. Morphological changes and cell apoptosis in rat OA cartilages were examined by safranin-O staining and terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) assay, respectively. The serum levels of tumor necrosis factor (TNF)-α and interleukin (IL)-2 in OA rats were assessed by enzyme-linked immunosorbent assay (ELISA). The expressions of SIRT1, cartilage matrix degradation-related proteins (matrix metalloproteinase (MMP)-9 and ADAMTS5), NF-κB signaling-related markers (p-p65/p65 and p-IκBα/IκBα), and cartilage matrix synthesis-related proteins (collagen II and aggrecan) in the OA cartilage were analyzed by western blot. As a result, acupuncture counteracted OA-associated upregulation of TNF-α, IL-2, cartilage matrix degradation-related proteins, and NF-κB signaling-related markers, morphological damage, apoptosis, SIRT1 downregulation, and loss of cartilage matrix synthesis-related proteins in rat articular cartilages. SIRT1 silencing reversed acupuncture-induced counteractive effects on the aforementioned OA-associated phenomena (except apoptosis, the experiment regarding which under SIRT1 silencing was not performed). Collectively, acupuncture inhibited chondrocyte apoptosis, inflammation, NF-κB signaling activation, and cartilage matrix degradation by upregulating SIRT1 expression to delay OA-associated cartilage degeneration.

scholarly journals Upper Zone of Growth Plate and Cartilage Matrix (UCMA) Levels in Patients with Chronic Kidney Disease

2020 ◽  
Vol 63 ◽  
Author(s):  
Hamza Malik Okuyan ◽  
Oğuzhan Özcan ◽  
Serdar Doğan ◽  
Abdullah Arpacı ◽  
Faruk Hilmi Turgut
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Growth Plate ◽  
Cartilage Matrix ◽  
And Cartilage

scholarly journals Upper zone of growth plate and cartilage matrix associated protein protects cartilage during inflammatory arthritis

2018 ◽  
Vol 20 (1) ◽  
Author(s):  
Fritz Seuffert ◽  
Daniela Weidner ◽  
Wolfgang Baum ◽  
Georg Schett ◽  
Michael Stock
Keyword(s):  
Growth Plate ◽  
Inflammatory Arthritis ◽  
Cartilage Matrix ◽  
And Cartilage

Cleft Lip Rhinoplasty: The Role of Bone and Cartilage Grafts

1989 ◽  
Vol 16 (1) ◽  
pp. 177-186 ◽  
Author(s):  
Fernando Ortiz Monasterio ◽  
Ernesto J. Ruas
Keyword(s):  
Cleft Lip ◽  
Cartilage Grafts ◽  
And Cartilage

Dual role of B7 costimulation in obesity-related non-alcoholic steatohepatitis (NASH) and metabolic dysregulation

2014 ◽  
Vol 122 (03) ◽  
Author(s):  
A Chatzigeorgiou ◽  
R Garcia-Martin ◽  
KJ Chung ◽  
I Alexaki ◽  
A Klotzsche-von Ameln ◽  
...  
Keyword(s):  
Dual Role ◽  
Alcoholic Steatohepatitis ◽  
Metabolic Dysregulation
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