scholarly journals Xenotransplantation of human dental pulp stem cells in platelet‑rich plasma for the treatment of full‑thickness articular cartilage defects in a rabbit model

2019 ◽  
Author(s):  
Ricardo Yanasse ◽  
Roger de L�bio ◽  
Leonardo Marques ◽  
Josianne Fukasawa ◽  
Rosimeire Segato ◽  
...  
Keyword(s):  
Stem Cells ◽  
Articular Cartilage ◽  
Dental Pulp ◽  
Platelet Rich Plasma ◽  
Rabbit Model ◽  
Dental Pulp Stem Cells ◽  
Cartilage Defects ◽  
Full Thickness ◽  
Human Dental Pulp ◽  
Articular Cartilage Defects

Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage Defects in a Rabbit Model

2016 ◽  
Vol 34 (11) ◽  
pp. 543-549 ◽  
Author(s):  
Reza Fekrazad ◽  
Mohamadreza Baghaban Eslaminejad ◽  
Arman M. Shayan ◽  
Katayoun A.M. Kalhori ◽  
Fatemeh Mashhadi Abbas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Rabbit Model ◽  
Cartilage Defects ◽  
Articular Cartilage Defects

scholarly journals Development of a Novel Large Animal Model to Evaluate Human Dental Pulp Stem Cells for Articular Cartilage Treatment

2018 ◽  
Vol 14 (5) ◽  
pp. 734-743 ◽  
Author(s):  
Tiago Lazzaretti Fernandes ◽  
Kazunori Shimomura ◽  
Andre Asperti ◽  
Carla Cristina Gomes Pinheiro ◽  
Heloísa Vasconcellos Amaral Caetano ◽  
...  
Keyword(s):  
Stem Cells ◽  
Animal Model ◽  
Articular Cartilage ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Large Animal Model ◽  
Large Animal ◽  
Cartilage Treatment ◽  
Human Dental Pulp

Autologous Matrix-Induced Chondrogenesis vs Microfracture with PRP for Chondral Lesions of the Knee in a Rabbit Model

2018 ◽  
Vol 69 (4) ◽  
pp. 894-900 ◽  
Author(s):  
Pal Fodor ◽  
Raluca Fodor ◽  
Arpad Solyom ◽  
Cornel Catoi ◽  
Flaviu Tabaran ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Platelet Rich Plasma ◽  
Cartilage Damage ◽  
Rabbit Model ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Full Thickness ◽  
Complete Filling ◽  
Articular Cartilage Damage

Currently, microfracturing is the most commonly used cartilage repair procedure in cartilage defects. Our aim was to study the mechanism of in vivo cartilage repair in case of full-thickness articular cartilage damage of the knee using a three-dimensional matrix implanted without any preseeded cells in the defect. We also investigated whether platelet-rich plasma application after microfracture procedure of the knee is associated with improved outcome compared with traditional microfracture treatment alone in a rabbit model. Histological examination of the chondral defects, revealed the largest amount of new tissue with hyaline-like cartilage features in Hyalofast group. At 12 weeks from implantation of the Hyalofast scaffold demonstrated complete filling of the defect with hyaline cartilage in admixture with the scaffold and bone metaplasia in the deepest areas. In the PRP group, complete filling of the defect with an admixture of fibrous and hyaline-like cartilage tissue appeared with a discreet tendency of endochondral ossification. We confirmed the superiority of the autologous matrix-induced chondrogenesis compared to microfracture and PRP or microfracture alone in case of full-thickness articular cartilage damage of the knee.

scholarly journals The Effect of Calcium Gluconate on Platelet Rich Plasma Activation for VEGF-A Expression of Human Dental Pulp Stem Cells

2021 ◽  
Author(s):  
Anggraini Margono ◽  
Dini Asrianti Bagio ◽  
Indah Julianto ◽  
Endang Suprastiwi
Keyword(s):  
Stem Cells ◽  
Calcium Gluconate ◽  
Dental Pulp ◽  
Platelet Rich Plasma ◽  
Elisa Test ◽  
Dental Pulp Stem Cells ◽  
Initial Release ◽  
Growth Method ◽  
Human Dental Pulp ◽  
Endothelial Growth Factor

Abstract Objective Platelet-rich plasma (PRP) activation is an important factor in triggering the initial release of blood-derived growth factors from platelets. Vascular endothelial growth factor-A (VEGF-A) can be expressed by human dental pulp stem cells (hDPSCs) and plays an important role in dental pulp angiogenesis. The aim of this study is to analyze the effects of calcium gluconate on PRP activation in hDPSC VEGF-A expression. Materials and Methods Two types of PRP and their corresponding activators were analyzed in this study: PRP (activated using calcium chloride/CaCl2) and PRP-T (activated using CaCl2 with the addition of 10% calcium gluconate). hDPSCs were obtained by using an out-growth method (DPSCs-OG), and harvest between the fifth and sixth passages, then cultured in three different media groups: control, PRP, and PRP-T, which were planted in 96 wells (5 × 103 each well). The VEGF-A expression of hDPSCs was analyzed by using an ELISA test and observed at 24, 48, and 72 hours. Statistical Analysis This study was performed by using one-way ANOVA (p < 0.05) test. Results There were significant differences between all groups (p < 0.05) at 48 and 72 hours of observations, and no significant differences in the PRP and PRP-T groups at 48 and 72 hours of observations (p > 0.05). Conclusion PRP and PRP-T were equally effective in inducing VEGF-A expression of hDPSCs.

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