Effects of octopamine on fluid secretion by isolated salivary glands of a feeding ixodid tick

1985 ◽  
Vol 2 (2) ◽  
pp. 217-226 ◽  
Author(s):  
Tom Pannabecker ◽  
Glen R. Needham
Keyword(s):  
Salivary Glands ◽  
Fluid Secretion ◽  
Ixodid Tick

Properties of Na, K-ATPase from the salivary glands of the ixodid tick Amblyomma hebraeum

1980 ◽  
Vol 58 (6) ◽  
pp. 1052-1059 ◽  
Author(s):  
B. Rutti ◽  
B. Schlunegger ◽  
W. Kaufman ◽  
A. Aeschlimann
Keyword(s):  
Enzyme Activity ◽  
Salivary Glands ◽  
Fluid Secretion ◽  
Ixodid Tick ◽  
Rich Source ◽  
Amblyomma Hebraeum ◽  
Fundamental Properties ◽  
High Concentrations

Tick (Amblyomma hebraeum) salivary glands are a rich source of Na,K-ATPase (EC 3.6.1.3), the fundamental properties of which are similar to those of Na,K-ATPases from other sources. Inhibition of the enzyme by ouabain is quantitatively similar to the inhibition of fluid secretion by this drug. Harmaline at high concentrations also inhibited the Na,K-ATPase. The nucleotides GTP, ITP, and UTP were utilized as substrates, but all were less effective than ATP. Noradrenaline, dopamine, and phenoxybenzamine, all at concentrations known to influence fluid secretion in vitro, had no effect on enzyme activity.

The influence of various factors on fluid secretion by in vitro salivary glands of ixodid Ticks

1976 ◽  
Vol 64 (3) ◽  
pp. 727-742
Author(s):  
W. Kaufman
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Culture Medium ◽  
Dry Weight ◽  
Fluid Secretion ◽  
Ixodid Tick ◽  
Ixodid Ticks ◽  
Mammalian Tissue ◽  
Stimulating Factor

1. Salivary glands of the female ixodid tick, Dermacentor andersoni, secrete fluid in vitro when bathed in a slightly modified version of the mammalian tissue culture medium ‘TC 199′. 2. Rate of salivation in vitro increases with progression of feeding, but there is no comparable increase in dry weight of the salivary glands during the early phase of engorgement. Engorged ticks secreted at only 25% the rate of 90–250 mg ticks, indicating that salivary gland degeneration has already begun in the very early post-engorgement stage. 3. A salivary gland stimulating factor can be detected in the nervous system but not in other tissues. 4. Male salivary glands secrete at only 1/20th the rate of female glands. Thus males probably do not use their salivary glands as osmoregulatory organs. 5. From the uniform lack of response to ACh and uniform response to DA in 7 ixodid tick species, it is suggested that the control of salivation is similar throughout the ixodid family.

Studies on the mechanism of fluid secretion by isolated salivary glands of Calliphora

1976 ◽  
Vol 64 (2) ◽  
pp. 311-322
Author(s):  
M. J. Berridge ◽  
B. D. Lindley ◽  
W. T. Prince
Keyword(s):  
Salivary Glands ◽  
Apical Membrane ◽  
Potassium Concentration ◽  
Basal Membrane ◽  
Fluid Secretion ◽  
Sodium Permeability ◽  
Bathing Medium ◽  
Major Cation ◽  
Diuretic Agent ◽  
External Potassium

1. Potassium is the major cation in the secretion of the salivary glands of Calliphora and is necessary for full secretory rates. 2. Other ions (rubidium and sodium) can support secretion in the absence of potassium. 39. During stimulation with 5-HT a Nernst plot of the basal membrane potential has a slope of 53 mV for a tenfold change in external potassium concentration and the slope at rest deviates from this over the range I-20 mM external potassium. 4. Hyperpolarization of the basal membrane by 5-HT is abolished if the chloride in the bathing medium is replaced by isethionate. 5. The diuretic agent amiloride inhibits fluid secretion by a mechanism which may include a reduction in calcium entry in addition to its recognized effect on sodium permeability. 6. A model is proposed in which fluid secretion is driven by the active transport of potassium across the apical membrane with chloride following passively.

The Effects Of Derivatives Of Adenosine 3',5'-Monophosphate On Fluid Secretion By The Salivary Glands Of Calliphora

1973 ◽  
Vol 59 (3) ◽  
pp. 595-606
Author(s):  
M. J. BERRIDGE
Keyword(s):  
Cyclic Amp ◽  
Salivary Glands ◽  
Cyclic Nucleotides ◽  
Fluid Secretion ◽  
Receptor Interaction ◽  
Base Region ◽  
Adenine Ring ◽  
Derivatives Of ◽  
Ribose Sugar

1. The nature of the cyclic AMP-receptor interaction was analysed by testing a range of cyclic nucleotides on the isolated salivary glands of adult blowflies. 2. All compounds containing modifications in the region of ribose or the phosphate ring were inactive. One compound, adenosine 3',5'-phosphorothioate, appeared to compete with cyclic AMP. 3. A number of nucleotides with alterations restricted to the base region of the molecule could stimulate secretion equally as well as cyclic AMP. 4. These observations indicate that during the action of cyclic AMP the phosphate ring and ribose sugar are critical whereas the adenine ring plays a relatively unspecific role.

Fluid secretion by isolated tick salivary glands dependent on an intact cytoskeleton

1994 ◽  
Vol 24 (4) ◽  
pp. 563-567 ◽  
Author(s):  
William J. Lamoreaux ◽  
Glen R. Needham ◽  
Lewis B. Coons
Keyword(s):  
Salivary Glands ◽  
Fluid Secretion ◽  
Tick Salivary Glands

scholarly journals Neural Involvement in the Control of Salivary Gland Degeneration in the Ixodid Tick Amblyomma Hebraeum

1984 ◽  
Vol 109 (1) ◽  
pp. 281-290
Author(s):  
ROBERT A. HARRIS ◽  
WILLIAM R. KAUFMAN
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Crude Extract ◽  
Seminal Receptacle ◽  
Ixodid Tick ◽  
Ixodid Ticks ◽  
Secretory Function ◽  
Amblyomma Hebraeum ◽  
Gland Function ◽  
Male Genital

1. We have developed a simple, quantitative assay to monitor salivary gland function in ixodid ticks. 2. Salivary glands of engorged (1.0-3.0g), or partially engorged (0.40-1.0g), Amblyomma hebraeum Koch lose virtually all secretory function within 4 days after engorgement. 3. Salivary glands from partially-fed ticks (0.20-0.30 g) lose 75 % of their secretory ability by 4 days post-removal, but retain this level of function for at least another 11 days. 4. Partially-fed ticks (0.20-0.30 g) removed from the host for 4 days and then allowed to re-attach and resume feeding for a further 2 days, recover much of their lost function. 5. Cutting the opisthosomal nerves of partially-fed ticks (0.40-1.0 g) inhibits salivary gland degeneration. 6. Excising the seminal receptacle from partially-fed ticks (0.40-1.0 g) inhibits salivary gland degeneration. Replacement of the seminal receptacle permits salivary gland degeneration to proceed normally. The factor from the seminal receptacle appears to be distinct from tick salivary gland degeneration factor (TSGDF; Harris & Kaufman, 1981). 7. Injecting a crude extract of male genital tracts into large, partially-fed ticks (0.40-1.0 g), which had had their seminal receptacles removed, caused virtually complete salivary gland degeneration. Such ticks, when injected with an extract of male salivary glands, showed no such degeneration. This suggests that the factor associated with mating originates in the male and is transferred to the female during copulation.

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