Heat stress hardening of oriental armyworms is induced by a transient elevation of reactive oxygen species during sublethal stress

2017 ◽  
Vol 96 (3) ◽  
pp. e21421 ◽  
Author(s):  
Takashi Matsumura ◽  
Hitoshi Matsumoto ◽  
Yoichi Hayakawa
Keyword(s):  
Reactive Oxygen Species ◽  
Heat Stress ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Sublethal Stress ◽  
Transient Elevation

Reactive oxygen species induced by heat stress during grain filling of rice ( Oryza sativa L.) are involved in occurrence of grain chalkiness

2017 ◽  
Vol 216 ◽  
pp. 52-57 ◽  
Author(s):  
Chetphilin Suriyasak ◽  
Keisuke Harano ◽  
Koichiro Tanamachi ◽  
Kazuhiro Matsuo ◽  
Aina Tamada ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Oryza Sativa ◽  
Heat Stress ◽  
Oryza Sativa L ◽  
Reactive Oxygen ◽  
Grain Filling ◽  
Oxygen Species ◽  
Grain Chalkiness

170 The effects of linoleic and linolenic acid supplementation on the in vitro maturation of pig oocytes in a heat-stressed environment

2019 ◽  
Vol 31 (1) ◽  
pp. 209
Author(s):  
M. Mentler ◽  
J. Current ◽  
B. Whitaker
Keyword(s):  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Heat Stress ◽  
Linolenic Acid ◽  
Oocyte Maturation ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Treatment Groups ◽  
Intracellular Glutathione

Elevated environmental temperatures induce heat stress, which can cause a depression in fertility and early embryonic development. Fatty acids initiate an endergonic reaction that is able to absorb cellular heat, causing a decrease in intracellular temperature. Supplementing linoleic and linolenic acids to the maturation medium of pig oocytes at elevated temperatures reduces the effects of heat stress-induced damage during fertilization and embryonic development. However, the mechanism of action of fatty acids during oocyte maturation is unknown. Therefore, the objective of this study was to minimize heat stress-induced damage and characterise the intracellular oocyte mechanisms. Oocyte maturation media was supplemented with linoleic and linolenic acid during oocyte maturation at either 38.5 or 41.5°C. Oocytes (n=3094, r=5) were supplemented with 50μM linoleic acid, 50μM linolenic acid, 25μM of both, or 50μM of both during 40 to 44h of maturation and then evaluated for the formation of reactive oxygen species (n=239), intracellular glutathione concentrations (n=1005), glutathione peroxidase (n=1005), catalase (n=987), and superoxide dismutase (n=863) activities. Data were analysed using ANOVA with the main effects including treatment, well, and replicate. There were no significant differences between the treatment groups matured at 38.5°C when comparing reactive oxygen species generation. Supplementation of linoleic or linolenic acid significantly decreased (P<0.05) reactive oxygen species generation in oocytes matured at 41.5°C compared with no supplementation at the same temperature. Supplementation of linoleic or linolenic acid or both significantly increased (P<0.05) intracellular glutathione concentrations compared with no supplementation at 38.5°C (23.37±1.23 pmol/oocyte) and 41.5°C (10.42±1.01 pmol/oocyte). There were no significant differences between the treatment groups matured at 38.5°C or 41.5°C when comparing glutathione peroxidase activity. Supplementation of linoleic or linolenic acid or both significantly increased (P<0.05) catalase and superoxide dismutase activities compared with no supplementation at 38.5°C and at 41.5°C. Superoxide dismutase activity was significantly higher (P<0.05) in oocytes matured at 41.5°C compared with those matured at 38.5°C. These results indicate that supplementing linoleic and linolenic acid to the maturation medium of pig oocytes at an elevated temperature reduces the effects of heat stress-induced damage by increasing intracellular glutathione concentrations and catalase and superoxide dismutase activities.

Cantharis 200c counters heat stress in germinating seeds of Vigna unguiculata (L.) Walp.

2021 ◽  
Vol 17 (3-4) ◽  
pp. 9-19
Author(s):  
Tandra Sarkar ◽  
Atheni Konar ◽  
Nirmal Chandra Sukul ◽  
Nirmal Chandra Sukul
Keyword(s):  
Reactive Oxygen Species ◽  
Heat Stress ◽  
Soluble Sugar ◽  
Reactive Oxygen ◽  
Thiobarbituric Acid ◽  
Membrane Lipid ◽  
Protein Accumulation ◽  
Oxygen Species ◽  
Negative Controls ◽  
Germinating Seeds

Background Thermal stress during early imbibitional phase of germination causes disruption of redox-homeostasis by increasing accumulation of ROS Reactive Oxygen species (assessed in terms of hydrogen peroxide) and significant reduction of antioxidative defense (assessed in terms of catalase and peroxidase) in germinating tissues of cowpea (Vigna ungiculata). It also induces oxidative damage to newly assembled membrane system by aggravating membrane lipid peroxidation [measured in terms of thiobarbituric acid reactive substances (TBARS)]. Cantharis is a homeopathic remedy used for treating burn injuries in humans. The objective is to see whether potentized Cantharis could ameliorate heat stress in germinating seeds of cowpea. Methodology Seeds, imbibed in water overnight, were divided into four groups (n=50/group): Control I (Water 200c), Control II (Ethanol 200c), treated with Cantharis 200c and untreated and unstressed. Water soaked seeds were dipped in control/drug solution for 5 min and then washed. Control II and Cantharis 200c were diluted with water 1:1000 to minimize the ethanol effect. Except the fourth group, all other groups were subjected to heat stress (450C for 8 hours). All the groups were allowed to germinate for 5 days in germination chamber at 25 ± 20C. Groups 1 and 2 (Control I and II) served as systematic negative controls throughout the experiment. Ten independent replications were performed for each group in parallel. All experiments were randomized and blinded. Results The systematic negative controls (I, II) did not produce any significant effect. The results in terms of germination, growth, soluble sugar, protein, accumulation of reactive oxygen species and loss of membrane permeability clearly exhibit that Cantharis 200c could mitigate heat stress significantly (p

Reactive oxygen species, heat stress and oxidative-induced mitochondrial damage. A review

2014 ◽  
Vol 30 (7) ◽  
pp. 513-523 ◽  
Author(s):  
Imen Belhadj Slimen ◽  
Taha Najar ◽  
Abdeljelil Ghram ◽  
Hajer Dabbebi ◽  
Moncef Ben Mrad ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Heat Stress ◽  
Reactive Oxygen ◽  
Mitochondrial Damage ◽  
Oxygen Species
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