The effect of trypan blue upon maternal protein metabolism and embryonic development

1959 ◽  
Vol 133 (3) ◽  
pp. 513-525 ◽  
Author(s):  
J. Langman ◽  
H. van Drunen
Keyword(s):  
Embryonic Development ◽  
Protein Metabolism ◽  
Trypan Blue

Effects of position in the uterus on fetal mortality and on response to trypan blue

Development ◽  
1967 ◽  
Vol 17 (3) ◽  
pp. 617-624
Author(s):  
Sidney L. Beck
Keyword(s):  
Embryonic Development ◽  
Blood Supply ◽  
Relative Position ◽  
Trypan Blue ◽  
Normal Development ◽  
Uterine Horn ◽  
Fetal Mortality ◽  
Uterine Environment ◽  
The Common

In a polytocous species such as the mouse, the relative position in the uterus that a fetus occupies may well have an effect upon the likelihood of normal development or indeed survival (Trasler, 1960; Woollam & Millen, 1962). It would seem desirable, therefore, especially since the mouse is so valuable a tool in studies of development (Grüneberg, 1963) and teratogenesis (Fave, 1964; Woollam, 1966), to learn as much as possible about the contribution of the uterine environment to embryonic development. It has been suggested that fetuses implanted in the portion of the uterine horn nearest the common uterus may have an increased tendency toward death or deformity when their dams are made anoxic experimentally during gestation (Woollam & Millen, 1962). These authors suggest that the variations in response to anoxia might be accounted for by differences in blood supply or different times of implantation in the different regions of the uterus.

scholarly journals INTRACELLULAR DISTRIBUTION OF CALCIUM IN DEVELOPING BREAST MUSCLE OF NORMAL AND DYSTROPHIC CHICKENS

1964 ◽  
Vol 23 (2) ◽  
pp. 241-252 ◽  
Author(s):  
Ethel Cosmos
Keyword(s):  
Embryonic Development ◽  
Protein Metabolism ◽  
Microsomal Fraction ◽  
Calcium Content ◽  
Intracellular Distribution ◽  
Differential Centrifugation ◽  
Maximal Level ◽  
Complete Exchange ◽  
A New Technique ◽  
Muscle Calcium

To follow the intracellular distribution of calcium in the breast muscles of developing chickens, Ca45 was injected into the albumen of predeveloped eggs. Since the embryos were grown in a radioactive medium, a complete exchange of the isotope for its non-radioactive counterpart in muscles was accomplished. Subcellular particulates of the muscle cells were separated by the method of differential centrifugation. Analysis of the separated fractions showed that in the muscles of the 13-day embryo, when the nuclear-myofibrillar ratio is high, 65 per cent of the muscle calcium is in the nuclei. With the increased synthesis of myofibrils, the nuclear-myofibrillar ratio decreases with a concomitant fall in radioactivity. Thus, calcium was not associated with the developing myofibrils. At the time of hatching, when myofibrils perform physiological work, the highest level of calcium is in the mitochondria. This suggests that the mitochondria play a key role in the physiological activities of calcium in the cell. The microsomal fraction reaches a maximal level of calcium when the adult composition of muscle is attained. Results of investigations on dystrophic muscles show changes in the calcium distribution of the fractions as early as the 3rd week of embryonic development, which are interpreted to indicate an alteration in the protein metabolism of the cell, or an early destruction of muscle tissue. Further, alterations in the calcium content of fractions which seem to regulate the movements of this ion in the cell are discussed. A new technique for homogenizing tissues from embryos of different ages is presented.

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

ADHD therapy with methylphenidate in mothers – a danger for embryonic development?

2014 ◽  
Vol 47 (06) ◽  
Author(s):  
N Bergemann ◽  
K Boyle ◽  
WE Paulus
Keyword(s):  
Embryonic Development

Effects of alimentary factors on intensity of protein metabolism in hens

2017 ◽  
Vol 19 (4) ◽  
pp. 24-30
Author(s):  
B. Ya. Kyryliv ◽  
◽  
A. V. Hunchak ◽  
Keyword(s):  
Protein Metabolism
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