scholarly journals Bioprinting a cell‐laden matrix for bone regeneration: A focused review

2020 ◽  
Vol 138 (8) ◽  
pp. 49888
Author(s):  
Farnaz Ghorbani ◽  
Dejian Li ◽  
Zeyuan Zhong ◽  
Melika Sahranavard ◽  
Zhi Qian ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
A Cell

scholarly journals Bone Regeneration in Defects Compromised by Radiotherapy

2009 ◽  
Vol 89 (1) ◽  
pp. 77-81 ◽  
Author(s):  
W.-W. Hu ◽  
B.B. Ward ◽  
Z. Wang ◽  
P.H. Krebsbach
Keyword(s):  
Bone Regeneration ◽  
Bone Development ◽  
Micro Ct ◽  
Mineral Density ◽  
Woven Bone ◽  
Before And After ◽  
Biomaterial Scaffolds ◽  
A Cell ◽  
Previously Treated ◽  
Freely Suspended

Because bone reconstruction in irradiated sites is less than ideal, we applied a regenerative gene therapy method in which a cell-signaling virus was localized to biomaterial scaffolds to regenerate wounds compromised by radiation therapy. Critical-sized defects were created in rat calvariae previously treated with radiation. Gelatin scaffolds containing lyophilized adenovirus encoding BMP-2 (AdBMP-2) or freely suspended AdBMP-2 were transplanted. Lyophilized AdBMP-2 significantly improved bone quality and quantity over free AdBMP-2. Bone mineral density was reduced after radiotherapy. Histological analyses demonstrated that radiation damage led to less bone regeneration. The woven bone and immature marrow formed in the radiated defects indicated that irradiation retarded normal bone development. Finally, we stored the scaffolds with lyophilized AdBMP-2 at −80°C to determine adenovirus stability. Micro-CT quantification demonstrated no significant differences between bone regeneration treated with lyophilized AdBMP-2 before and after storage, suggesting that virus-loaded scaffolds may be convenient for application as pre-made constructs.

Preparation of Silicon-Containing Poly(lactic acid)-Vaterite Hybrid Membranes

2010 ◽  
Vol 638-642 ◽  
pp. 670-674
Author(s):  
Akiko Obata ◽  
Takashi Wakita ◽  
Yoshio Ota ◽  
Toshihiro Kasuga
Keyword(s):  
Lactic Acid ◽  
Simulated Body Fluid ◽  
Bone Regeneration ◽  
Trace Amount ◽  
Body Fluid ◽  
Poly Lactic Acid ◽  
Osteogenic Cells ◽  
A Cell ◽  
Silicon Doped ◽  
Gbr Membrane

Microfiber meshes releasing a trace amount of silicon species were prepared by electrospinning silicon-doped vaterite (SiV) and poly(lactic acid) (PLA) hybrids for application to membranes for guided bone regeneration (GBR). A trace amount of silicon-species has been reported to enhance the mineralization and bone-forming abilities of osteogenic cells. The microfiber meshes prepared by electrospinning are regarded to be a useful candidate for the GBR membrane, because they have adequate flexibility and porosity for it. In this study, hydroxyapatite (HA)-forming abilities in simulated body fluid, silicon-releasabilities, compatibility with osteoblast-like cells of the prepared microfiber meshes were examined. The meshes were completely coated with HA after soaking in simulated body fluid for 1 day. The meshes coated with HA released 0.2 -0.7 mg/L of silicon species in a cell culture medium for 7 days. The cells elongated on the microfibers of the meshes and some of them entered the mesh after 1 day-culturing. The meshes are expected to provide an excellent substrate for bone regeneration and enhance bone-forming ability of the cells.

Bioengineered mussel glue incorporated with a cell recognition motif as an osteostimulating bone adhesive for titanium implants

2015 ◽  
Vol 3 (41) ◽  
pp. 8102-8114 ◽  
Author(s):  
Yun Kee Jo ◽  
Bong-Hyuk Choi ◽  
Cong Zhou ◽  
Jin-Soo Ahn ◽  
Sang Ho Jun ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Titanium Implants ◽  
Osteoblastic Cell ◽  
Cell Recognition ◽  
Cell Behaviors ◽  
Recognition Motif ◽  
A Cell

An engineered mussel glue MAP-RGD can be successfully used as a novel functional osteostimulating bone adhesive for titanium implants through improved osteoblastic cell behaviors, blood responses, and eventually enhanced bone regeneration.

Microscopic local stiffening in a supramolecular hydrogel network expedites stem cell mechanosensing in 3D and bone regeneration

2021 ◽  
Author(s):  
Weihao Yuan ◽  
Haixing Wang ◽  
Chao Fang ◽  
Yongkang Yang ◽  
Xingyu Xia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Bone Regeneration ◽  
Network Topology ◽  
Supramolecular Hydrogel ◽  
Hydrogel Network ◽  
A Cell ◽  
Cell Mechanosensing

A cell-adaptable hydrogel containing microscopically local stiffening structures shows a heterogeneous and hierarchical hydrogel network topology, thereby facilitating the 3D stellate spreading of stem cells and promoting the bone regeneration.

Nanofibrous Glass Tailored with Apatite-Fibronectin Interface for Bone Cell Stimulation

2008 ◽  
Vol 8 (6) ◽  
pp. 3013-3019 ◽  
Author(s):  
Hae-Won Kim ◽  
Hae-Hyoung Lee ◽  
Jonathan C. Knowles
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Bone Mineral ◽  
Bone Matrix ◽  
Bone Cell ◽  
Cell Stimulation ◽  
Adhesive Protein ◽  
Cell Adhesive ◽  
A Cell

Exploring a material with smart and biomimetic interface has great potential in the biomaterials and tissue engineering field. This paper reports a novel nanofibrous bone matrix that was developed to retain a cell-stimulating and bone-mimetic biointerface. The bone mineral, apatite, and the cell adhesive protein, fibronectin (FN), were hybridized on the interface of a bioactive glass nano-fibrous mesh, through the dissolution-and-reprecipitation process. The hybridized nanofibrous mesh showed significant improvement in the initial responses of the bone-derived cells. It is believed that this biomimetic and cell-stimulating nanofibrous mesh can be used as a potential bone regeneration matrix.

Biomimetic Nanosilica–Collagen Scaffolds for In Situ Bone Regeneration: Toward a Cell‐Free, One‐Step Surgery

2019 ◽  
Vol 31 (49) ◽  
pp. 1904341 ◽  
Author(s):  
Shao‐Jie Wang ◽  
Dong Jiang ◽  
Zheng‐Zheng Zhang ◽  
You‐Rong Chen ◽  
Zheng‐Dong Yang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Collagen Scaffolds ◽  
A Cell ◽  
One Step

scholarly journals Evaluation of bone regeneration in mandible large defect using undifferentiated adipose stem cells loaded on gelatin carrier: An animal model case study

2021 ◽  
Vol 15 (1) ◽  
pp. 22-29
Author(s):  
Ali Hossein Mesgarzadeh ◽  
Islam Nasiri ◽  
Seyedhosein Jarolmasjed ◽  
Mehran Naghibi ◽  
Hajar Shafaei
Keyword(s):  
Stem Cells ◽  
Ct Scan ◽  
Bone Regeneration ◽  
Bone Repair ◽  
Hounsfield Unit ◽  
Adipose Stem Cells ◽  
Control Group ◽  
Large Defect ◽  
Model Case ◽  
A Cell

Background. Large mandibular defects are considered difficult reconstructive challenges for oral and maxillofacial surgeons. Cell therapy, as an alternative technique, might increase the speed of bone regeneration. This study aimed to investigate bone regeneration in large defects of dog mandibles using allogenic adipose-derived stem cells on gelatin foam as a cell carrier. Methods. The tissue engineering phase consisted of the sampling of adult dogs’ adipose tissue that can easily be isolated from adipose stem cells (ASCs) of the dogs, ASCs were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, USA) with low glucose, containing 10% fetal bovine serum (FBS) (Sigma, USA) and 1% penicillin-streptomycin (Gibco, USA), with the characterization of dog ASCs and gelatin-transplanted ASCs. Six dogs were included in this experimental study in the next step and randomly assigned to the treatment and control groups. The samples in both groups underwent surgery under general anesthesia to create uniform 3-cm bony defects. The samples in both groups were reconstructed with titanium reconstruction plates and screws. A large bone gap filled with ASCs (5×106 ) was seeded on gelatin (ASCs) in the treatment group. In the control group, bony defects were filled with a cell delivery carrier without ASCs. Six months after transplantation, the animals’ mandibles were evaluated by CT scan imaging, and the results were quantified through the Hounsfield unit (HU). The data were analyzed with t-test. Results. Before transplantation, the nature of the stem cells was confirmed by the expression of CD44 and CD105 cell markers at 71.9% and 89.3%, respectively, and a lack of the CD45 cell marker expression at 2.2%. Evaluation of CT scan images showed significantly higher bone repair in the ASCs group (920.25±572.92 HU) than in the control group (-94.746± 08.42). Conclusion. The bone regeneration of the ASCs group was significantly higher than that in the control group.

Preliminary Study on Systematic Modeling and Optimal Control of Dual Delivery Nanocarriers for Bone Regeneration

2011 ◽  
Vol 282-283 ◽  
pp. 147-152
Author(s):  
Hui Ming Peng ◽  
Jia Wen Bian ◽  
Hong Wei Li
Keyword(s):  
Optimal Control ◽  
Drug Release ◽  
Bone Regeneration ◽  
Cell Response ◽  
Response Model ◽  
Input And Output ◽  
A Cell ◽  
Release Model ◽  
The Relationship ◽  
Systematic Modeling

This study is focused on the development of clinically applicable nanocarriers for bone regeneration by establishing a systematic modeling guided nanocarriers development methodology. Firstly a drug release model is built through different release mechanisms to predict the profiles of drugs released from nanospheres. Then a cell response model is built through multiple signaling pathways related to the released drugs to predict the relationship between the drug profiles and the terminal cell phenotypes. Finally the cell response model combined with the drug release model will be employed to optimally predict the relationship between the input and output of the complete model, to establish an entire system with tunable input and output, and finally by optimal control to guide and accelerate the design of the BMP-2 and vancomycin incorporated nanocarriers.

A Cell-Engineered Small Intestinal Submucosa-Based Bone Mimetic Construct for Bone Regeneration

2018 ◽  
Vol 24 (13-14) ◽  
pp. 1099-1111 ◽  
Author(s):  
Mei Li ◽  
Chi Zhang ◽  
Yuxing Mao ◽  
Yi Zhong ◽  
Jiyuan Zhao
Keyword(s):  
Bone Regeneration ◽  
Small Intestinal Submucosa ◽  
A Cell ◽  
Small Intestinal ◽  
Intestinal Submucosa
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