Random and aligned PLLA : PRGF electrospun scaffolds for regenerative medicine

Luis Díaz-Gómez; Florencia Montini Ballarin; Gustavo A. Abraham; Angel Concheiro; Carmen Alvarez-Lorenzo

doi:10.1002/app.41372

Composite Bioceramics/Polymer Electrospun Scaffolds for Regenerative Medicine

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.529-530.441 ◽

2012 ◽

Vol 529-530 ◽

pp. 441-446

Author(s):

Thomas Miramond ◽

Pascal Borget ◽

Caroline Colombeix ◽

Serge Baroth ◽

G. Daculsi

Keyword(s):

Cell Adhesion ◽

Calcium Phosphate ◽

Regenerative Medicine ◽

Tricalcium Phosphate ◽

Biphasic Calcium Phosphate ◽

Electrospun Scaffolds ◽

Mineral Particles

The main goal of this study was to succeed in the relevant association of well-known osteoconductive biphasic calcium phosphate (BCP) made of Hydroxyapatite (20% HA) and β-Tricalcium Phosphate (80% β-TCP) crystallographic phases and resorbable poly (L-lactide-co-D,L-lactide)(PLDLLA) 3D matrices synthesized by electrospinning. Two types of mineral particles were obtained, BCP new hollow granules, and classical BCP particles. It appeared that hollow shells/PLDLLA composite 3D matrices allowed higher cell adhesionin vitro,thanks to internal concavities and are promising scaffolds in terms of cell carrying.

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A one-step biofunctionalization strategy of electrospun scaffolds enables spatially selective presentation of biological cues

10.1101/850875 ◽

2019 ◽

Author(s):

Paul Wieringa ◽

Andre Girao ◽

Roman Truckenmuller ◽

Alexander Welle ◽

Silvestro Micera ◽

...

Keyword(s):

Extracellular Matrix ◽

Regenerative Medicine ◽

Dorsal Root ◽

Neurite Growth ◽

Electrospun Scaffolds ◽

Selective Functionalization ◽

Promising Strategy ◽

Reactive Groups ◽

Spatial Domains ◽

One Step

AbstractTo recapitulate the heterogeneous complexity of tissues in our body with synthetic mimics of the extracellular matrix (ECM), it is important to develop methods that can easily allow the selective functionalization of defined spatial domains. Here, we introduce a facile method to functionalize microfibrillar meshes with different reactive groups able to bind biological moieties in a one-step reaction. The resulting scaffolds proved to selectively support a differential neurite growth after being seeded with dorsal root ganglia. Considering the general principles behind the method developed, this is a promising strategy to realize enhanced biomimicry of native ECM for different regenerative medicine applications.

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Applications of Poly (caprolactone)-Based Nanofibre Electrospun Scaffolds in Tissue Engineering and Regenerative Medicine

Current Stem Cell Research & Therapy ◽

10.2174/1574888x15666201014145703 ◽

2020 ◽

Vol 15 ◽

Author(s):

Wei Zhang ◽

Tingting Weng ◽

Qiong Li ◽

Ronghua Jin ◽

Chuangang You ◽

...

Keyword(s):

Tissue Engineering ◽

Regenerative Medicine ◽

Biological Properties ◽

Future Research ◽

Natural Polymers ◽

Bioactive Substances ◽

Electrospun Scaffolds ◽

Recent Developments ◽

Poly Caprolactone

: Diseases, trauma, and injuries are highly prevalent conditions that lead to many critical tissue defects. Tissue engineering has great potentials to develop functional scaffolds that mimic natural tissue structures to improve or replace biological functions. In many kinds of technologies, electrospinning has received widespread attention for its outstanding functions, which is capable of producing nanofibre structures similar to the natural extracellular matrix. Amongst, the electrospinning of available biopolymers, poly (caprolactone) (PCL), has shown favorable outcomes for tissue regeneration applications. According to the characteristics of different tissues, PCL can be modified by altering the functional groups or combining with other materials such as synthetic polymers, natural polymers, and metal materials to improve its physicochemical, mechanical, and biological properties, making the electrospun scaffolds meet the requirements of different tissue engineering and regenerative medicine. Moreover, efforts have been made to modify nanofibres with several bioactive substances to provide cells with the necessary chemical cues and a more in vivo like environment. In this review, some recent developments in both the design and utility of electrospun PCL-based scaffolds in the fields of bone, cartilage, skin, tendon, ligament and nerve are highlighted, along with their potential impact on future research and clinical applications.

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A review of electrospinning manipulation techniques to direct fiber deposition and maximize pore size

Electrospinning ◽

10.1515/esp-2017-0002 ◽

2017 ◽

Vol 2 (1) ◽

pp. 46-61 ◽

Cited By ~ 20

Author(s):

Kevin P. Feltz ◽

Emily A. Growney Kalaf ◽

Chengpeng Chen ◽

R. Scott Martin ◽

Scott A. Sell

Keyword(s):

Magnetic Field ◽

Tissue Engineering ◽

Extracellular Matrix ◽

Regenerative Medicine ◽

Electrospinning Process ◽

Electrospun Scaffolds ◽

Alternative Techniques ◽

Fiber Deposition ◽

Medicine Community ◽

Field Manipulation

Abstract Electrospinning has been widely accepted for several decades by the tissue engineering and regenerative medicine community as a technique for nanofiber production. Owing to the inherent flexibility of the electrospinning process, a number of techniques can be easily implemented to control fiber deposition (i.e. electric/ magnetic field manipulation, use of alternating current, or air-based fiber focusing) and/or porosity (i.e. air impedance, sacrificial porogen/sacrificial fiber incorporation, cryo-electrospinning, or alternative techniques). The purpose of this review is to highlight some of the recent work using these techniques to create electrospun scaffolds appropriate for mimicking the structure of the native extracellular matrix, and to enhance the applicability of advanced electrospinning techniques in the field of tissue engineering.

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Recent Advances in Cell Electrospining of Natural and Synthetic Nanofibers for Regenerative Medicine

Drug Research ◽

10.1055/s-0043-125314 ◽

2018 ◽

Vol 68 (08) ◽

pp. 425-435 ◽

Cited By ~ 9

Author(s):

Reza Zamani ◽

Sedigheh Aval ◽

Younes Pilehvar-Soltanahmadi ◽

Kazem Nejati-Koshki ◽

Nosratollah Zarghami

Keyword(s):

Cell Proliferation ◽

Regenerative Medicine ◽

Electrospun Nanofibers ◽

Natural Structure ◽

Electrospinning Technique ◽

Electrospun Scaffolds ◽

Support Cell ◽

Potential Applications ◽

Directed Growth ◽

Natural And Synthetic

AbstractThe progression of nanotechnology provides opportunities to manipulate synthetic and natural materials to mimic the natural structure for tissue engineering applications. The electrospinning technique applies electrostatic principle to fabricate electrospun nanofibers. Nanofiber scaffolds are precisely similar to the native extracellular matrix (ECM) and support cell proliferation, adhesion, tendency to preserve their phenotypic shape and directed growth according to the nanofiber direction. This study reviewed both the natural and synthetic type of nanofibers and described the different properties used to trigger certain process in the tissue development. Also, the potential applications of electrospun scaffolds for regenerative medicine were summarized.

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