Synthesis of polyvinylidene fluoride (PVDF) membranes for protein binding: Effect of casting thickness

2012 ◽  
Vol 128 (5) ◽  
pp. 3438-3445 ◽  
Author(s):  
A. L. Ahmad ◽  
N. Ideris ◽  
B. S. Ooi ◽  
S. C. Low ◽  
A. Ismail
Keyword(s):  
Protein Binding ◽  
Polyvinylidene Fluoride ◽  
Binding Effect

Morphology and polymorph study of a polyvinylidene fluoride (PVDF) membrane for protein binding: Effect of the dissolving temperature

Desalination ◽  
2011 ◽  
Vol 278 (1-3) ◽  
pp. 318-324 ◽  
Author(s):  
A.L. Ahmad ◽  
N. Ideris ◽  
B.S. Ooi ◽  
S.C. Low ◽  
A. Ismail
Keyword(s):  
Protein Binding ◽  
Polyvinylidene Fluoride ◽  
Pvdf Membrane ◽  
Binding Effect

Evaluation of Protein Binding Effect on Local Disposition of Oxacillin in Rat Liver by a Two-compartment Dispersion Model

1990 ◽  
Vol 42 (9) ◽  
pp. 632-636 ◽  
Author(s):  
YOSHITAKA YANO ◽  
KIYOSHI YAMAOKA ◽  
TOSHIOMI MINAMIDE ◽  
TERUMICHI NAKAGAWA ◽  
HISASHI TANAKA
Keyword(s):  
Protein Binding ◽  
Rat Liver ◽  
Dispersion Model ◽  
Binding Effect

Influence of the physicochemical environment on membrane-protein binding: investigation using a fabricated PVDF membrane

2015 ◽  
Vol 35 (4) ◽  
pp. 339-347
Author(s):  
Abdul L. Ahmad ◽  
Norhidayah Ideris ◽  
Ooi Boon Seng ◽  
Low Siew Chun ◽  
Asma Ismail
Keyword(s):  
Membrane Protein ◽  
Protein Binding ◽  
Polyvinylidene Fluoride ◽  
Pvdf Membrane ◽  
Ph Values ◽  
Symmetric Structure ◽  
Binding Mechanisms

Abstract The correlation between the behavior of a polyvinylidene fluoride (PVDF) membrane toward protein binding at different physicochemical environments was investigated. The PVDF membrane was first fabricated and characterized to understand its morphological, polymorph and intrinsic characteristics. The results confirmed that the membrane had a microporous, symmetric structure and high hydrophobicity and electronegativity. The membrane was further tested under different physicochemical environments by modifying the type of protein and pH medium used. The results showed that different proteins and pH values contribute to different membrane-protein binding mechanisms. Overall, the results of PVDF membrane-protein binding were satisfactory and demonstrated the ability of the membrane to capture various proteins or reagents.

scholarly journals Investigating Membrane Material and Morphology for Development of Lateral Flow Biosensor

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
R. Shaimi ◽  
A. L. Ahmad ◽  
S. C. Low
Keyword(s):  
Drinking Water ◽  
Protein Binding ◽  
Polyvinylidene Fluoride ◽  
Lateral Flow ◽  
Cellulose Acetate Membrane ◽  
Binding Ability ◽  
Nylon Membrane ◽  
Sensing Applications ◽  
High Protein Binding ◽  
Experimental Findings

Development of membrane for bio–sensing applications, such as the detection of pathogens in drinking water for epidemics control has a huge global impact especially for public health. Membrane that applied in the bio–sensing devices should fulfill requirements such as high binding ability, fast lateral wicking time and low background staining. This paper explores the selection of membranes, including Nitrocellulose membrane (NC), Cellulose Acetate membrane (CA), Polyvinylidene Fluoride membrane (PVDF) and Nylon membrane for the detection of pathogens in water in the most efficient and rapid way. Membrane modification using glutaraldehyde enable the achieved of higher sensitivity of protein binding. Experimental findings (FTIR, porosity, membrane’s binding ability and lateral wicking time) verified the most suitable membrane for bio-sensing application. Among the tested membranes, NC appeared as the most suitable lateral flow membrane as its performances of high protein binding ability and fast lateral wicking time. Throughout this study, we showed the correlation of membrane’s material and morphology to its performances for pathogens detection in drinking water.

Amitriptyline plasma protein binding: Effect of plasma pH and relevance to clinical overdose

1986 ◽  
Vol 4 (2) ◽  
pp. 121-125 ◽  
Author(s):  
M.Andrew Levitt ◽  
John B. Sullivan ◽  
S.Michael Owens ◽  
Leah Burnham ◽  
Paul R. Finley
Keyword(s):  
Protein Binding ◽  
Plasma Protein Binding ◽  
Plasma Protein ◽  
Binding Effect

scholarly journals Phosphorylation in Protein-Protein Binding: Effect on Stability and Function

Structure ◽  
2011 ◽  
Vol 19 (12) ◽  
pp. 1807-1815 ◽  
Author(s):  
Hafumi Nishi ◽  
Kosuke Hashimoto ◽  
Anna R. Panchenko
Keyword(s):  
Protein Binding ◽  
Binding Effect ◽  
And Function

scholarly journals Electrospun Fibers of Polybutylene Succinate/Graphene Oxide Composite for Syringe-Push Protein Absorption Membrane

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2042
Author(s):  
Nuankanya Sathirapongsasuti ◽  
Anuchan Panaksri ◽  
Sani Boonyagul ◽  
Somchai Chutipongtanate ◽  
Nuttapol Tanadchangsaeng
Keyword(s):  
Graphene Oxide ◽  
Protein Binding ◽  
Polyvinylidene Fluoride ◽  
Membrane Filtration ◽  
Binding Capacity ◽  
Low Cost ◽  
Morphological Properties ◽  
Binding Property ◽  
Oxide Composite ◽  
Polybutylene Succinate

The adsorption of proteins on membranes has been used for simple, low-cost, and minimal sample handling of large volume, low protein abundance liquid samples. Syringe-push membrane absorption (SPMA) is an innovative way to process bio-fluid samples by combining a medical syringe and protein-absorbable membrane, which makes SPMA a simple, rapid protein and proteomic analysis method. However, the membrane used for SPMA is only limited to commercially available protein-absorbable membrane options. To raise the method’s efficiency, higher protein binding capacity with a lower back pressure membrane is needed. In this research, we fabricated electrospun polybutylene succinate (PBS) membrane and compared it to electrospun polyvinylidene fluoride (PVDF). Rolling electrospinning (RE) and non-rolling electrospinning (NRE) were employed to synthesize polymer fibers, resulting in the different characteristics of mechanical and morphological properties. Adding graphene oxide (GO) composite does not affect their mechanical properties; however, electrospun PBS membrane can be applied as a filter membrane and has a higher pore area than electrospun PVDF membrane. Albumin solution filtration was performed using all the electrospun filter membranes by the SPMA technique to measure the protein capture efficiency and staining of the protein on the membranes, and these membranes were compared to the commercial filter membranes—PVDF, nitrocellulose, and Whatman no. 1. A combination of rolling electrospinning with graphene oxide composite and PBS resulted in two times more captured protein when compared to commercial membrane filtration and more than sixfold protein binding than non-composite polymer. The protein staining results further confirmed the enhancement of the protein binding property, showing more intense stained color in compositing polymer with GO.

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