Statistical optimization of palm oil hydrolysis by Pseudomonas aeruginosa PseA lipase

2010 ◽  
Vol 6 (1) ◽  
pp. 147-153 ◽  
Author(s):  
Ruchi Gaur ◽  
Sunil Kumar Khare
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Palm Oil ◽  
Statistical Optimization ◽  
Oil Hydrolysis

Palm oil hydrolysis by lipase from Candida cylindracea immobilized on zeolite type Y

1998 ◽  
Vol 22 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Zorica Knezevic ◽  
Ljiljana Mojovic ◽  
Borivoje Adnadjevic
Keyword(s):  
Palm Oil ◽  
Candida Cylindracea ◽  
Zeolite Type ◽  
Oil Hydrolysis

Biosurfactants Production by Pseudomonas aeruginosa FR Using Palm Oil

2006 ◽  
Vol 131 (1-3) ◽  
pp. 727-737 ◽  
Author(s):  
Fernando J. S. Oliveira ◽  
Leonardo Vazquez ◽  
Norberto P. de Campos ◽  
Francisca P. de Franña
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Palm Oil

Biosurfactants production by Pseudomonas aeruginosa FR using palm oil

1996 ◽  
Vol 131 (1-3) ◽  
pp. 727-737
Author(s):  
Fernando J. S. Oliveira ◽  
Leonardo Vazquez ◽  
Norberto P. Campos ◽  
Francisca P. França
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Palm Oil

scholarly journals Karakterisasi PHA yang dihasilkan oleh Pseudomonas aeruginosa dan Bacillus subtilis yang ditumbuhkan dalam media limbah cair pabrik kelapa sawit Characterization of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis inoculated in palm oil mill effluent (POME) media

2016 ◽  
Vol 82 (2) ◽  
Author(s):  
Irma KRESNAWATY ◽  
Agustin Sri MULYATNI ◽  
Deden Dewantara ERIS ◽  
Haryo Tejo PRAKOSO
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Palm Oil ◽  
Ft Ir ◽  
Pha Production ◽  
Palm Oil Mill ◽  
Scanning Electron

AbstractThe difficulties in processing of petroleum-based plastic waste had encouraged the development of biodegradable plastics polyhydroxyalkanoate (PHA). Researchers isolated the PHA-producing microorganisms from various sources to obtain new species with high PHA production capability. In addition, the high cost of PHA production might be overcome by using carbon-rich waste, such as palm oil mill effluent (POME). This research conducted characterization of produced PHA and optimization of PHA production in POME. In previous research, three potential isolates were obtained, which are one Pseudomonas aeruginosa isolate and two Bacillus subtilis isolates. Analysis of Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) showed the presence of PHA accumu-lation within the bacterial cell. The results of Spectra of Fourier Transform Infra Red Spectroscopy (FT-IR) revealed differences in C-C and C-H alipathic regions of PHA produced by Pseudomonas aeruginosa and Bacillus subtilis. Optimum production of PHA was obtained using POME at concentration of 50-25% during 6 days of incubation time in an enriched media pretreatment.Abstrak Sulitnya pengolahan limbah plastik berbasis minyak bumi mendorong pengembangan plastik biodegradable poli-hidroksialkanoat (PHA). Beberapa peneliti mengisolasi mikroorganisme penghasil  PHA dari berbagai sumber karena diharapkan akan diperoleh spesies baru dengan kemampuan produksi PHA yang tinggi. Selain itu kendala tingginya biaya produksi PHA dapat diatasi dengan peman-faatan limbah yang kaya akan  karbon, seperti limbah cair pabrik kelapa sawit (LCPKS). Pada penelitian ini dilakukan karakterisasi PHA yang dihasilkan dan optimasi produksi PHA pada LCPKS. Pada penelitian sebelumnya telah diperoleh  tiga isolat potensial, yaitu : satu isolat  Pseudo-monas aeruginosa dan dua isolat  Bacillus subtilis.  Analisis Scanning Electron Microscopy (SEM) dan Transmission Electon Microscopy (TEM) menunjukkan adanya akumulasi PHA di dalam sel bakteri. Dari hasil analisis FT-IR disimpulkan bahwa senyawa PHA yang dihasilkan Pseudo-monas aeruginosa  berbeda dengan  Bacillus subtilisyang ditandai   perbedaan   pada  spectra   gugus  C-C  dan   C-H alifatik.  Produksi optimum PHA diperoleh pada konsentrasi LCPKS 50-25%, waktu inkubasi  enam hari dan optima-lisasi pertumbuhan pada media kaya di awal untuk mening-katkan populasi mikroba.

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