Collaborative Biosynthesis of a Class of Bioactive Azaphilones by Two Separate Gene Clusters Containing Four PKS/NRPSs with Transcriptional Crosstalk in Fungi

2020 ◽  
Vol 59 (11) ◽  
pp. 4349-4353 ◽  
Author(s):  
Xuenian Huang ◽  
Wei Zhang ◽  
Shen Tang ◽  
Suhui Wei ◽  
Xuefeng Lu
Keyword(s):  
Gene Clusters ◽  
Separate Gene

scholarly journals The Chlamydia pneumoniae Type III Secretion-Related lcrH Gene Clusters Are Developmentally Expressed Operons

2005 ◽  
Vol 187 (22) ◽  
pp. 7853-7856 ◽  
Author(s):  
Scot P. Ouellette ◽  
Yasser M. AbdelRahman ◽  
Robert J. Belland ◽  
Gerald I. Byrne
Keyword(s):  
Type Iii Secretion ◽  
Sigma Factor ◽  
Chlamydia Pneumoniae ◽  
Developmental Regulation ◽  
Gene Clusters ◽  
Secretion System ◽  
Structural Components ◽  
Separate Gene ◽  
Type Iii ◽  
Regulation Mechanisms

ABSTRACT Two chlamydial homologues of the Yersinia lcrH chaperone for type III secretion system structural components are present within separate gene clusters. Quantitative transcriptional analyses demonstrated that each cluster is differentially regulated and expressed as an operon using major sigma factor elements, suggesting the presence of more elaborate developmental regulation mechanisms in chlamydiae.

scholarly journals Two Separate Gene Clusters Encode the Biosynthetic Pathway for the Meroterpenoids Austinol and Dehydroaustinol inAspergillus nidulans

2012 ◽  
Vol 134 (10) ◽  
pp. 4709-4720 ◽  
Author(s):  
Hsien-Chun Lo ◽  
Ruth Entwistle ◽  
Chun-Jun Guo ◽  
Manmeet Ahuja ◽  
Edyta Szewczyk ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Gene Clusters ◽  
Separate Gene

scholarly journals At least two separate gene clusters are involved in albicidin production by Xanthomonas albilineans.

1996 ◽  
Vol 178 (15) ◽  
pp. 4590-4596 ◽  
Author(s):  
P C Rott ◽  
L Costet ◽  
M J Davis ◽  
R Frutos ◽  
D W Gabriel
Keyword(s):  
Gene Clusters ◽  
Separate Gene ◽  
Xanthomonas Albilineans

scholarly journals Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa

Microbiology ◽  
2005 ◽  
Vol 151 (11) ◽  
pp. 3649-3656 ◽  
Author(s):  
Birgit Höschle ◽  
Volker Gnau ◽  
Dieter Jendrossek
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Affinity Chromatography ◽  
Molecular Mass ◽  
Gene Clusters ◽  
Apparent Molecular Mass ◽  
Catabolic Pathway ◽  
Fingerprint Analysis ◽  
Genome Database ◽  
Key Enzymes ◽  
Separate Gene

Pseudomonas aeruginosa is able to grow on acyclic monoterpenes (citronellol, citronellate, geraniol and geranylate), and on other methyl-branched compounds such as leucine or isovalerate. The catabolic pathway of citronellol (Atu, acyclic terpene utilization) enters that of leucine/isovalerate (Liu, leucine and isovalerate utilization) at the level of methylcrotonyl-CoA. Key enzymes of the combined pathways are geranyl-CoA carboxylase (GCase) and methylcrotonyl-CoA carboxylase (MCase). In this study, isovalerate-grown cells specifically expressed MCase (apparent molecular mass of the biotin-containing subunit, 74 kDa) only, and the GCase biotin-containing subunit (71 kDa) was not detected. Citronellol- or citronellate-grown cells produced both carboxylases. Biotin-dependent proteins were purified from crude extracts by avidin-affinity chromatography, and assigned to the corresponding coding genes by trypsin fingerprint analysis. The two subunits of MCase corresponded to liuB/liuD (PA2014/PA2012) of the P. aeruginosa genome database, and atuC/atuF (PA2888/PA2891) encoded GCase subunits. This finding is contrary to that reported by others. The identified genes are part of two separate gene clusters [liuRABCDE (PA2011–PA2016) and atuABCDEFGH (PA2886–PA2893)] that are thought to encode most of the genes of the Atu and Liu pathways.

Collaborative Biosynthesis of a Class of Bioactive Azaphilones by Two Separate Gene Clusters Containing Four PKS/NRPSs with Transcriptional Crosstalk in Fungi

2020 ◽  
Vol 132 (11) ◽  
pp. 4379-4383 ◽  
Author(s):  
Xuenian Huang ◽  
Wei Zhang ◽  
Shen Tang ◽  
Suhui Wei ◽  
Xuefeng Lu
Keyword(s):  
Gene Clusters ◽  
Separate Gene

The Estimation of prostate specific antigen (PSA) concentrations in patients with prostatitis by fully automated ELISA technique.

2020 ◽  
Vol 3 (11) ◽  
pp. 1100-1104
Author(s):  
Hussein Naeem Aldhaheri ◽  
Ihsan Edan AlSaimary ◽  
Murtadha Mohammed ALMusafer
Keyword(s):  
Personal Information ◽  
Receptor Gene ◽  
Specific Antigen ◽  
Gene Clusters ◽  
Control Group ◽  
P Value ◽  
Group Data ◽  
Elisa Technique ◽  
And Control

      The Aim of this study was to determine Immunogenetic expression of  Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men from Basrah and Maysan provinces. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected. This study shows the effect of PSA level in patients with prostatitis and control group, with P-value <0.0001 therefore the study shows a positive significant between elevated PSA levels and Prostatitis.

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Phylogeny of actin and tubulin gene homologs in diverse eukaryotic species

2019 ◽  
Vol 79 (01S) ◽  
Author(s):  
Pawan Kumar Jayaswal ◽  
Asheesh Shanker ◽  
Nagendra Kumar Singh
Keyword(s):  
Evolutionary Relationship ◽  
Genomic Data ◽  
Gene Clusters ◽  
Species Trees ◽  
Model Species ◽  
Tubulin Gene ◽  
Tubulin Genes ◽  
Eukaryotic Species ◽  
Relationship Of ◽  
Insight Into

Actin and tubulin are cytoskeleton proteins, which are important components of the celland are conserved across species. Despite their crucial significance in cell motility and cell division the distribution and phylogeny of actin and tubulin genes across taxa is poorly understood. Here we used publicly available genomic data of 49 model species of plants, animals, fungi and Protista for further understanding the distribution of these genes among diverse eukaryotic species using rice as reference. The highest numbers of rice actin and tubulin gene homologs were present in plants followed by animals, fungi and Protista species, whereas ten actin and nine tubulin genes were conserved in all 49 species. Phylogenetic analysis of 19 actin and 18 tubulin genes clustered them into four major groups each. One each of the actin and tubulin gene clusters was conserved across eukaryotic species. Species trees based on the conserved actin and tubulin genes showed evolutionary relationship of 49 different taxa clustered into plants, animals, fungi and Protista. This study provides a phylogenetic insight into the evolution of actin and tubulin genes in diverse eukaryotic species.

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