Ultrasensitive Detection of Salmonella and Listeria monocytogenes by Small‐Molecule Chemiluminescence Probes

2019 ◽  
Vol 58 (30) ◽  
pp. 10361-10367 ◽  
Author(s):  
Michal Roth‐Konforti ◽  
Ori Green ◽  
Mario Hupfeld ◽  
Lars Fieseler ◽  
Nadine Heinrich ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Small Molecule ◽  
Ultrasensitive Detection

Nanoarchitectonics of Small Molecule and DNA for Ultrasensitive Detection of Mercury

2016 ◽  
Vol 8 (44) ◽  
pp. 30362-30371 ◽  
Author(s):  
M. Pandeeswar ◽  
Satyaprasad P. Senanayak ◽  
T. Govindaraju
Keyword(s):  
Small Molecule ◽  
Ultrasensitive Detection

Ultrasensitive Detection ofSalmonellaandListeria monocytogenesby Small‐Molecule Chemiluminescence Probes

2019 ◽  
Vol 131 (30) ◽  
pp. 10469-10475 ◽  
Author(s):  
Michal Roth‐Konforti ◽  
Ori Green ◽  
Mario Hupfeld ◽  
Lars Fieseler ◽  
Nadine Heinrich ◽  
...  
Keyword(s):  
Small Molecule ◽  
Ultrasensitive Detection

A WS2 nanosheet-based nanosensor for the ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule-linked DNA and Nt.BstNBI-assisted recycling amplification

2016 ◽  
Vol 4 (30) ◽  
pp. 5161-5166 ◽  
Author(s):  
Jia Chen ◽  
Cunji Gao ◽  
Abul K. Mallik ◽  
Hongdeng Qiu
Keyword(s):  
Small Molecule ◽  
Protein Interaction ◽  
Ultrasensitive Detection ◽  
Recycling Amplification

A novel, ultrasensitive and specific fluorescent nanosensor for the detection of small molecule–protein interaction based on the terminal protection of small molecule-linked DNA and Nt.BstNBI-assisted recycling amplification was reported.

scholarly journals The Listeria monocytogenes σB Regulon and Its Virulence-Associated Functions Are Inhibited by a Small Molecule

mBio ◽  
2011 ◽  
Vol 2 (6) ◽  
Author(s):  
M. Elizabeth Palmer ◽  
Soraya Chaturongakul ◽  
Martin Wiedmann ◽  
Kathryn J. Boor
Keyword(s):  
Bacillus Subtilis ◽  
Listeria Monocytogenes ◽  
Small Molecule ◽  
Sigma Factor ◽  
Pathogen Transmission ◽  
Bile Salt Hydrolase ◽  
Alternative Sigma Factor ◽  
Bacterial Survival ◽  
Gram Positive ◽  
Content Type

ABSTRACTThe stress-responsive alternative sigma factor σBis conserved across diverse Gram-positive bacterial genera. InListeria monocytogenes, σBregulates transcription of >150 genes, including genes contributing to virulence and to bacterial survival under host-associated stress conditions, such as those encountered in the human gastrointestinal lumen. An inhibitor ofL. monocytogenesσBactivity was identified by screening ~57,000 natural and synthesized small molecules using a high-throughput cell-based assay. The compound fluoro-phenyl-styrene-sulfonamide (FPSS) (IC50= 3.5 µM) downregulated the majority of genes previously identified as members of the σBregulon inL. monocytogenes10403S, thus generating a transcriptional profile comparable to that of a 10403S ΔsigBstrain. Specifically, of the 208 genes downregulated by FPSS, 75% had been identified previously as positively regulated by σB. Downregulated genes included key virulence and stress response genes, such asinlA,inlB,bsh,hfq,opuC, andbilE. From a functional perspective, FPSS also inhibitedL. monocytogenesinvasion of human intestinal epithelial cells and bile salt hydrolase activity. The ability of FPSS to inhibit σBactivity in bothL. monocytogenesandBacillus subtilisindicates its utility as a specific inhibitor of σBacross multiple Gram-positive genera.IMPORTANCEThe σBtranscription factor regulates expression of genes responsible for bacterial survival under changing environmental conditions and for virulence; therefore, this alternative sigma factor is important for transmission ofL. monocytogenesand other Gram-positive bacteria. Regulation of σBactivity is complex and tightly controlled, reflecting the key role of this factor in bacterial metabolism. We present multiple lines of evidence indicating that fluoro-phenyl-styrene-sulfonamide (FPSS) specifically inhibits activity of σBacross Gram-positive bacterial genera, i.e., in bothListeria monocytogenesandBacillus subtilis. Therefore, FPSS is an important new tool that will enable novel approaches for exploring complex regulatory networks inL. monocytogenesand other Gram-positive pathogens and for investigating small-molecule applications for controlling pathogen transmission.

scholarly journals A Small-Molecule Screen Identifies the Antipsychotic Drug Pimozide as an Inhibitor of Listeria monocytogenes Infection

2008 ◽  
Vol 53 (2) ◽  
pp. 756-764 ◽  
Author(s):  
Linda A. Lieberman ◽  
Darren E. Higgins
Keyword(s):  
Listeria Monocytogenes ◽  
Antipsychotic Drug ◽  
Small Molecule ◽  
Bacterial Infections ◽  
Actin Polymerization ◽  
Bacterial Species ◽  
Inhibitory Effect ◽  
Phagocytic Cells ◽  
Murine Bone Marrow ◽  
Serovar Typhimurium

ABSTRACT We developed a screening procedure to identify small-molecule compounds that altered infection by Listeria monocytogenes to gain insights into bacterial/host cellular processes required for intracellular pathogenesis. A small-molecule library of 480 compounds with known biological functions was screened, and 21 compounds that altered the L. monocytogenes infection of murine bone marrow-derived macrophages (BMM) were identified. The identified compounds affected various cellular functions, such as actin polymerization, kinase/phosphatase activity, calcium signaling, and apoptosis. Pimozide, an FDA-approved drug used to treat severe Tourette's syndrome and schizophrenia, was further examined and shown to decrease the bacterial uptake and vacuole escape of L. monocytogenes in BMM. The inhibitory effect of pimozide on internalization was not specific for L. monocytogenes, as the phagocytosis of other bacterial species (Bacillus subtilis, Salmonella enterica serovar Typhimurium, and Escherichia coli K12) was significantly inhibited in the presence of pimozide. The invasion and cell-to-cell spread of L. monocytogenes during the infection of nonprofessional phagocytic cells also was decreased by pimozide treatment. Although pimozide has been reported to be an antagonist of mammalian cell calcium channels, the infection of BMM in a calcium-free medium did not relieve the inhibitory effects of pimozide on L. monocytogenes infection. Our results provide a generalizable screening approach for identifying small-molecule compounds that affect cellular pathways that are required for intracellular bacterial pathogenesis. We also have identified pimozide, a clinically approved antipsychotic drug, as a compound that may be suitable for further development as a therapeutic for intracellular bacterial infections.

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