Ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification

2013 ◽  
Vol 49 (78) ◽  
pp. 8854 ◽  
Author(s):  
Guohua Zhou ◽  
Xian Zhang ◽  
Xinghu Ji ◽  
Zhike He
Keyword(s):  
Small Molecule ◽  
Protein Interaction ◽  
Ultrasensitive Detection ◽  
Exo Iii ◽  
Recycling Amplification

A WS2 nanosheet-based nanosensor for the ultrasensitive detection of small molecule–protein interaction via terminal protection of small molecule-linked DNA and Nt.BstNBI-assisted recycling amplification

2016 ◽  
Vol 4 (30) ◽  
pp. 5161-5166 ◽  
Author(s):  
Jia Chen ◽  
Cunji Gao ◽  
Abul K. Mallik ◽  
Hongdeng Qiu
Keyword(s):  
Small Molecule ◽  
Protein Interaction ◽  
Ultrasensitive Detection ◽  
Recycling Amplification

A novel, ultrasensitive and specific fluorescent nanosensor for the detection of small molecule–protein interaction based on the terminal protection of small molecule-linked DNA and Nt.BstNBI-assisted recycling amplification was reported.

Exonuclease III-assisted substrate fragment recycling amplification strategy for ultrasensitive detection of uranyl by a multipurpose DNAzyme

RSC Advances ◽  
2016 ◽  
Vol 6 (110) ◽  
pp. 108662-108667 ◽  
Author(s):  
Jin-Xiu Cao ◽  
Yong-Sheng Wang ◽  
Jin-Hua Xue ◽  
Yan-Qin Huang ◽  
Ming-Hui Li ◽  
...  
Keyword(s):  
Ultrasensitive Detection ◽  
Exonuclease Iii ◽  
Exo Iii ◽  
Recycling Amplification ◽  
Amplification Strategy

Substrate fragment cleaved by UO22+ hybridizes with SSP6 to form dsDNA, triggering substrate fragment recycling amplification by Exo III.

scholarly journals Development of a Novel High-Throughput Screen and Identification of Small-Molecule Inhibitors of the Gα–RGS17 Protein–Protein Interaction Using AlphaScreen

2011 ◽  
Vol 16 (8) ◽  
pp. 869-877 ◽  
Author(s):  
Duncan I. Mackie ◽  
David L. Roman
Keyword(s):  
Small Molecule ◽  
High Throughput ◽  
Protein Interaction ◽  
High Throughput Screening ◽  
Drug Targets ◽  
Screening Method ◽  
Fold Increase ◽  
Rgs Proteins ◽  
High Throughput Screen ◽  
Protein Protein Interaction

In this study, the authors used AlphaScreen technology to develop a high-throughput screening method for interrogating small-molecule libraries for inhibitors of the Gαo–RGS17 interaction. RGS17 is implicated in the growth, proliferation, metastasis, and the migration of prostate and lung cancers. RGS17 is upregulated in lung and prostate tumors up to a 13-fold increase over patient-matched normal tissues. Studies show RGS17 knockdown inhibits colony formation and decreases tumorigenesis in nude mice. The screen in this study uses a measurement of the Gαo–RGS17 protein–protein interaction, with an excellent Z score exceeding 0.73, a signal-to-noise ratio >70, and a screening time of 1100 compounds per hour. The authors screened the NCI Diversity Set II and determined 35 initial hits, of which 16 were confirmed after screening against controls. The 16 compounds exhibited IC50 <10 µM in dose–response experiments. Four exhibited IC50 values <6 µM while inhibiting the Gαo–RGS17 interaction >50% when compared to a biotinylated glutathione-S-transferase control. This report describes the first high-throughput screen for RGS17 inhibitors, as well as a novel paradigm adaptable to many other RGS proteins, which are emerging as attractive drug targets for modulating G-protein-coupled receptor signaling.

Ultrasensitive fluorometric biosensor based on Ti3C2 MXenes with Hg2+-triggered exonuclease III-assisted recycling amplification

The Analyst ◽  
2021 ◽  
Author(s):  
Liling Lu ◽  
Xiao Han ◽  
Jingwen Lin ◽  
Yingxin Zhang ◽  
Minghao Qiu ◽  
...  
Keyword(s):  
Two Dimensional ◽  
Exonuclease Iii ◽  
Exo Iii ◽  
Recycling Amplification ◽  
Fluorescence Quencher

Herein a rapid and sensitive fluorometric bioanalysis platform for mercury(II) (Hg2+) detection was innovatively developed using ultrathin two-dimensional MXenes (Ti3C2) as fluorescence quencher and Hg2+-induced exonuclease III (Exo III)-assisted target...

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