Avoiding Thiol Compound Interference: A Nanoplatform Based on High-Fidelity Au-Se Bonds for Biological Applications

2018 ◽  
Vol 57 (19) ◽  
pp. 5306-5309 ◽  
Author(s):  
Bo Hu ◽  
Fanpeng Kong ◽  
Xiaonan Gao ◽  
Lulu Jiang ◽  
Xiaofeng Li ◽  
...  
Keyword(s):  
High Fidelity ◽  
Biological Applications ◽  
Thiol Compound

Avoiding Thiol Compound Interference: A Nanoplatform Based on High-Fidelity Au-Se Bonds for Biological Applications

2018 ◽  
Vol 130 (19) ◽  
pp. 5404-5407 ◽  
Author(s):  
Bo Hu ◽  
Fanpeng Kong ◽  
Xiaonan Gao ◽  
Lulu Jiang ◽  
Xiaofeng Li ◽  
...  
Keyword(s):  
High Fidelity ◽  
Biological Applications ◽  
Thiol Compound

scholarly journals Basis for discrimination by engineered CRISPR/Cas9 enzymes

2019 ◽  
Author(s):  
Mu-Sen Liu ◽  
Shanzhong Gong ◽  
Helen-Hong Yu ◽  
Kyungseok Jung ◽  
Kenneth A. Johnson ◽  
...  
Keyword(s):  
High Fidelity ◽  
Dna Unwinding ◽  
Biological Applications ◽  
Wild Type ◽  
Guide Rna ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Target Dna ◽  
Rate Limiting

AbstractCRISPR/Cas9 is a programmable genome editing tool that has been widely used for biological applications. While engineered Cas9s have been reported to increase discrimination against off-target cleavage compared with wild type Streptococcus pyogenes (SpCas9) in vivo, the mechanism for enhanced specificity has not been extensively characterized. To understand the basis for improved discrimination against off-target DNA containing important mismatches at the distal end of the guide RNA, we performed kinetic analyses on the high-fidelity (Cas9-HF1) and hyper-accurate (HypaCas9) engineered Cas9 variants. While DNA unwinding is the rate-limiting step for on-target cleavage by SpCas9, we show that chemistry is seriously impaired by more than 100-fold for the high-fidelity variants. The high-fidelity variants improve discrimination by slowing the rate of chemistry without increasing the rate of DNA rewinding—the kinetic partitioning favors release rather than cleavage of a bound off-target substrate because chemistry is slow. Further improvement in discrimination may require engineering increased rates of dissociation of off-target DNA.

scholarly journals Thermoplastic microfluidic devices for targeted chemical and biological applications

RSC Advances ◽  
2017 ◽  
Vol 7 (5) ◽  
pp. 2884-2889 ◽  
Author(s):  
Dan Voicu ◽  
Gabriella Lestari ◽  
Yihe Wang ◽  
Michael DeBono ◽  
Minseok Seo ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Hot Embossing ◽  
High Fidelity ◽  
Biological Applications ◽  
Cost Efficient

Photolithography and hot embossing offers the capability of cost-efficient and high-fidelity fabrication of polymer microfluidic devices.

How SIMS microscopy can be used in medicine

1992 ◽  
Vol 50 (2) ◽  
pp. 1602-1603
Author(s):  
Philippe Fragu
Keyword(s):  
Mass Spectrometry ◽  
Biomedical Applications ◽  
Secondary Ion Mass Spectrometry ◽  
Chemical Elements ◽  
Biological Applications ◽  
The Past ◽  
Potential Source ◽  
Secondary Ion ◽  
Chemical Integrity ◽  
Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

Using the Theory of Planned Behavior to Predict Faking in Selection Exercises Varying in Fidelity

2018 ◽  
Vol 17 (3) ◽  
pp. 155-160 ◽  
Author(s):  
Daniel Dürr ◽  
Ute-Christine Klehe
Keyword(s):  
Theory Of Planned Behavior ◽  
Planned Behavior ◽  
Predictive Validity ◽  
Group Discussion ◽  
Role Play ◽  
Selection Procedure ◽  
High Fidelity ◽  
Selection Research ◽  
Play Group

Abstract. Faking has been a concern in selection research for many years. Many studies have examined faking in questionnaires while far less is known about faking in selection exercises with higher fidelity. This study applies the theory of planned behavior (TPB; Ajzen, 1991 ) to low- (interviews) and high-fidelity (role play, group discussion) exercises, testing whether the TPB predicts reported faking behavior. Data from a mock selection procedure suggests that candidates do report to fake in low- and high-fidelity exercises. Additionally, the TPB showed good predictive validity for faking in a low-fidelity exercise, yet not for faking in high-fidelity exercises.

The effects of a high fidelity vibro-kinetic multisensory experience on implicit and explicit brand recognition.

2019 ◽  
Vol 12 (1) ◽  
pp. 18-33 ◽  
Author(s):  
Horea Pauna ◽  
Pierre-Majorique Léger ◽  
Sylvain Sénécal ◽  
Marc Fredette ◽  
Élise Labonté-Lemoyne ◽  
...  
Keyword(s):  
High Fidelity ◽  
Brand Recognition ◽  
Implicit And Explicit ◽  
Multisensory Experience

Preparation of Graphene Quantum Dots and Their Biological Applications

2016 ◽  
Vol 31 (4) ◽  
pp. 337 ◽  
Author(s):  
SUN Xiao-Dan ◽  
LIU Zhong-Qun ◽  
YAN Hao
Keyword(s):  
Quantum Dots ◽  
Graphene Quantum Dots ◽  
Biological Applications
Sign in / Sign up

Export Citation Format

Share Document