scholarly journals Optochemical Control of Biological Processes in Cells and Animals

2018 ◽  
Vol 57 (11) ◽  
pp. 2768-2798 ◽  
Author(s):  
Nicholas Ankenbruck ◽  
Taylor Courtney ◽  
Yuta Naro ◽  
Alexander Deiters
Keyword(s):  
Biological Processes ◽  
In Cells

Mitochondria-targeting fluorescent sensor for on-off-on response to Cu2+ and ATP in cells and zebrafish

The Analyst ◽  
2021 ◽  
Author(s):  
Wan Sun ◽  
Guofeng Liu ◽  
Mingqiong Tong ◽  
Haozhan Wang ◽  
Shuhan Liu
Keyword(s):  
Adenosine Triphosphate ◽  
Fluorescent Sensor ◽  
Biological Processes ◽  
Fluorescent Molecule ◽  
Cupric Ion ◽  
Mitochondria Targeting ◽  
In Cells

Cupric ion (Cu2+) and adenosine triphosphate (ATP) are functionally important in mitochondria and play essential roles in many important biological processes. In this work, a mitochondria-targeting fluorescent molecule Mito-A was...

Interrogating the Transcriptome with Metabolically Incorporated Ribonucleosides

2021 ◽  
Author(s):  
Ralph Kleiner
Keyword(s):  
Biological Processes ◽  
Biochemical Mechanisms ◽  
In Cells

RNA is a central player in biological processes, but there remain major gaps in our understanding of transcriptomic processes and the underlying biochemical mechanisms regulating RNA in cells. A powerful...

Glycan Labeling and Analysis in Cells and In Vivo

2021 ◽  
Vol 14 (1) ◽  
pp. 363-387
Author(s):  
Bo Cheng ◽  
Qi Tang ◽  
Che Zhang ◽  
Xing Chen
Keyword(s):  
Chemical Modification ◽  
Biological Function ◽  
Biological Processes ◽  
Affinity Tags ◽  
Functional Roles ◽  
Antibody Conjugates ◽  
Living Organisms ◽  
In Cells

As one of the major types of biomacromolecules in the cell, glycans play essential functional roles in various biological processes. Compared with proteins and nucleic acids, the analysis of glycans in situ has been more challenging. Herein we review recent advances in the development of methods and strategies for labeling, imaging, and profiling of glycans in cells and in vivo. Cellular glycans can be labeled by affinity-based probes, including lectin and antibody conjugates, direct chemical modification, metabolic glycan labeling, and chemoenzymatic labeling. These methods have been applied to label glycans with fluorophores, which enables the visualization and tracking of glycans in cells, tissues, and living organisms. Alternatively, labeling glycans with affinity tags has enabled the enrichment of glycoproteins for glycoproteomic profiling. Built on the glycan labeling methods, strategies enabling cell-selective and tissue-specific glycan labeling and protein-specific glycan imaging have been developed. With these methods and strategies, researchers are now better poised than ever to dissect the biological function of glycans in physiological or pathological contexts.

scholarly journals The Minimum Information about a Molecular Interaction CAusal STatement (MI2CAST)

2020 ◽  
Author(s):  
Vasundra Touré ◽  
Steven Vercruysse ◽  
Marcio Luis Acencio ◽  
Ruth C Lovering ◽  
Sandra Orchard ◽  
...  
Keyword(s):  
Molecular Interaction ◽  
Regulatory Networks ◽  
Building Blocks ◽  
Supplementary Information ◽  
Biological Processes ◽  
Causal Interaction ◽  
End User ◽  
Minimum Information ◽  
Causal Statement ◽  
In Cells

Abstract Motivation A large variety of molecular interactions occurs between biomolecular components in cells. When a molecular interaction results in a regulatory effect, exerted by one component onto a downstream component, a so-called ‘causal interaction’ takes place. Causal interactions constitute the building blocks in our understanding of larger regulatory networks in cells. These causal interactions and the biological processes they enable (e.g. gene regulation) need to be described with a careful appreciation of the underlying molecular reactions. A proper description of this information enables archiving, sharing and reuse by humans and for automated computational processing. Various representations of causal relationships between biological components are currently used in a variety of resources. Results Here, we propose a checklist that accommodates current representations, called the Minimum Information about a Molecular Interaction CAusal STatement (MI2CAST). This checklist defines both the required core information, as well as a comprehensive set of other contextual details valuable to the end user and relevant for reusing and reproducing causal molecular interaction information. The MI2CAST checklist can be used as reporting guidelines when annotating and curating causal statements, while fostering uniformity and interoperability of the data across resources. Availability and implementation The checklist together with examples is accessible at https://github.com/MI2CAST/MI2CAST Supplementary information Supplementary data are available at Bioinformatics online.

A review: the trend of progress about pH probes in cell application in recent years

The Analyst ◽  
2017 ◽  
Vol 142 (1) ◽  
pp. 30-41 ◽  
Author(s):  
Yongkang Yue ◽  
Fangjun Huo ◽  
Songyi Lee ◽  
Caixia Yin ◽  
Juyoung Yoon
Keyword(s):  
Intracellular Ph ◽  
Body Fluids ◽  
The Body ◽  
Biological Processes ◽  
Acid Base ◽  
Ph Values ◽  
Ph Probes ◽  
In Cells

Intracellular pH values are some of the most important factors that govern biological processes and the acid–base homeostasis in cells, body fluids and organs sustains the normal operations of the body.

scholarly journals Molecular probes for cellular imaging of post-translational proteoforms

2022 ◽  
Author(s):  
Surased Suraritdechachai ◽  
Benya Lakkanasirorat ◽  
Chayasith Uttamapinant
Keyword(s):  
Signaling Pathways ◽  
Cellular Imaging ◽  
Molecular Probes ◽  
Biological Processes ◽  
Deep Insight ◽  
Post Translational Modification ◽  
And Function ◽  
Insight Into ◽  
In Cells

Specific post-translational modification (PTM) states of a protein affect its property and function; understanding their dynamics in cells would provide deep insight into diverse signaling pathways and biological processes. However,...

Label-free hyperspectral microscopy for scatter imaging of biological processes in cells (Conference Presentation)

2016 ◽  
Author(s):  
Jeeseong C. Hwang ◽  
Aniruddha Ray ◽  
Philip P. Cheney ◽  
Bonghwan Chon ◽  
Ji Youn Lee ◽  
...  
Keyword(s):  
Biological Processes ◽  
Label Free ◽  
In Cells

scholarly journals Profilin and formin constitute a pacemaker system for robust actin filament growth

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Johanna Funk ◽  
Felipe Merino ◽  
Larisa Venkova ◽  
Lina Heydenreich ◽  
Jan Kierfeld ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Actin Filament ◽  
Growth Rates ◽  
Actin Filaments ◽  
Mammalian Cells ◽  
Biological Processes ◽  
Muscle Actin ◽  
Cell Morphogenesis ◽  
Actin Networks ◽  
In Cells

The actin cytoskeleton drives many essential biological processes, from cell morphogenesis to motility. Assembly of functional actin networks requires control over the speed at which actin filaments grow. How this can be achieved at the high and variable levels of soluble actin subunits found in cells is unclear. Here we reconstitute assembly of mammalian, non-muscle actin filaments from physiological concentrations of profilin-actin. We discover that under these conditions, filament growth is limited by profilin dissociating from the filament end and the speed of elongation becomes insensitive to the concentration of soluble subunits. Profilin release can be directly promoted by formin actin polymerases even at saturating profilin-actin concentrations. We demonstrate that mammalian cells indeed operate at the limit to actin filament growth imposed by profilin and formins. Our results reveal how synergy between profilin and formins generates robust filament growth rates that are resilient to changes in the soluble subunit concentration.

scholarly journals Determination of biological processes regulated by HOXA9 in cells derived from Cervical, Uterine and its influence on the immune response.

2015 ◽  
Vol 6 ◽  
Author(s):  
Alvarado-Ruiz Liliana ◽  
Aguilar Lemarroy Adriana ◽  
Bravo Cuellar Alejandro ◽  
Jave-Suarez Luis
Keyword(s):  
Immune Response ◽  
Biological Processes ◽  
In Cells
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