Coumarin-Based Fluorogenic Probes for No-Wash Protein Labeling

2014 ◽  
Vol 53 (50) ◽  
pp. 13785-13788 ◽  
Author(s):  
Yingche Chen ◽  
Christopher M. Clouthier ◽  
Kelvin Tsao ◽  
Miroslava Strmiskova ◽  
Hugo Lachance ◽  
...  
Keyword(s):  
Protein Labeling ◽  
Fluorogenic Probes

Development of an effective protein-labeling system based on smart fluorogenic probes

2019 ◽  
Vol 24 (4) ◽  
pp. 443-455 ◽  
Author(s):  
Shahi Imam Reja ◽  
Masafumi Minoshima ◽  
Yuichiro Hori ◽  
Kazuya Kikuchi
Keyword(s):  
Protein Labeling ◽  
Fluorogenic Probes ◽  
Labeling System

Sydnone-coumarins as clickable turn-on fluorescent sensors for molecular imaging

2018 ◽  
Vol 54 (76) ◽  
pp. 10758-10761 ◽  
Author(s):  
Elodie Decuypère ◽  
Margaux Riomet ◽  
Antoine Sallustrau ◽  
Sarah Bregant ◽  
Robert Thai ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Fluorescent Sensors ◽  
Protein Labeling ◽  
Turn On ◽  
Fluorogenic Probes ◽  
Coumarin Compounds

Sydnone-coumarin compounds are interesting turn-on fluorogenic probes for protein labeling.

Coumarin-Based Fluorogenic Probes for No-Wash Protein Labeling

2014 ◽  
Vol 126 (50) ◽  
pp. 14005-14008 ◽  
Author(s):  
Yingche Chen ◽  
Christopher M. Clouthier ◽  
Kelvin Tsao ◽  
Miroslava Strmiskova ◽  
Hugo Lachance ◽  
...  
Keyword(s):  
Protein Labeling ◽  
Fluorogenic Probes

scholarly journals Correction: A systematic study of protein labeling by fluorogenic probes using cysteine targeting vinyl sulfone-cyclooctyne tags

2016 ◽  
Vol 14 (38) ◽  
pp. 9158-9158
Author(s):  
B. Söveges ◽  
T. Imre ◽  
T. Szende ◽  
Á. L. Póti ◽  
G. B. Cserép ◽  
...  
Keyword(s):  
Systematic Study ◽  
Protein Labeling ◽  
Vinyl Sulfone ◽  
Fluorogenic Probes

Correction for ‘A systematic study of protein labeling by fluorogenic probes using cysteine targeting vinyl sulfone-cyclooctyne tags’ by B. Söveges, et al., Org. Biomol. Chem., 2016, 14, 6071–6078.

SNAP-tag fluorogenic probes for wash free protein labeling

2017 ◽  
Vol 28 (10) ◽  
pp. 1911-1915 ◽  
Author(s):  
Shuang Leng ◽  
Qing-Long Qiao ◽  
Yue Gao ◽  
Lu Miao ◽  
Wu-Guo Deng ◽  
...  
Keyword(s):  
Protein Labeling ◽  
Free Protein ◽  
Fluorogenic Probes

Sydnone-based turn-on fluorogenic probes for no-wash protein labeling and in-cell imaging

2019 ◽  
Vol 55 (31) ◽  
pp. 4582-4585 ◽  
Author(s):  
Lucie Plougastel ◽  
Manas R. Pattanayak ◽  
Margaux Riomet ◽  
Sarah Bregant ◽  
Antoine Sallustrau ◽  
...  
Keyword(s):  
Cell Imaging ◽  
Cycloaddition Reaction ◽  
Protein Labeling ◽  
Turn On ◽  
Fluorogenic Probes

Fluorogenic sydnone-based turn-on probes allow efficient labeling of proteins and cell imaging through a bioorthogonal strained promoted sydnone–alkyne cycloaddition reaction.

A systematic study of protein labeling by fluorogenic probes using cysteine targeting vinyl sulfone-cyclooctyne tags

2016 ◽  
Vol 14 (25) ◽  
pp. 6071-6078 ◽  
Author(s):  
B. Söveges ◽  
T. Imre ◽  
T. Szende ◽  
Á. L. Póti ◽  
G. B. Cserép ◽  
...  
Keyword(s):  
Systematic Study ◽  
Click Reaction ◽  
Protein Labeling ◽  
Vinyl Sulfone ◽  
P Protein ◽  
Fluorogenic Probes

Protein labeling by cycloocytynylated vinyl sulfone linkers is fast and thiol-selective, and subsequent click reaction with fluorogenic azides generates intensive fluorescence.

No-Wash Protein Labeling with Designed Fluorogenic Probes and Application to Real-Time Pulse-Chase Analysis

2012 ◽  
Vol 134 (3) ◽  
pp. 1623-1629 ◽  
Author(s):  
Shin Mizukami ◽  
Shuji Watanabe ◽  
Yuri Akimoto ◽  
Kazuya Kikuchi
Keyword(s):  
Real Time ◽  
Protein Labeling ◽  
Fluorogenic Probes ◽  
Time Pulse

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

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