Photoactivated in situ Generation of Near Infrared Cyanines for Spatiotemporally Controlled Fluorescence Imaging in Living Cells

2021 ◽  
Author(s):  
Shu Wang
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Living Cells ◽  
In Situ Generation

scholarly journals Using Scuba for In Situ Determination of Chlorophyll Distributions in Corals by Underwater Near Infrared Fluorescence Imaging

2020 ◽  
Vol 8 (1) ◽  
pp. 53
Author(s):  
Thomas Oh ◽  
Jittiwat Sermsripong ◽  
Barry W. Hicks
Keyword(s):  
Fluorescence Imaging ◽  
Large Scale ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Fluorescence Emission ◽  
Florida Keys ◽  
Nir Fluorescence ◽  
Novel Method ◽  
Near Infrared Fluorescence Imaging

Studies reporting quantitation and imaging of chlorophyll in corals using visible fluorescent emission in the red near 680 nm can suffer from competing emission from other red-emitting pigments. Here, we report a novel method of selectively imaging chlorophyll distributions in coral in situ using only the near infrared (NIR) fluorescence emission from chlorophyll. Commercially available equipment was assembled that allowed the sequential imaging of visible, visible-fluorescent, and NIR-fluorescent pigments on the same corals. The relative distributions of chlorophyll and fluorescent proteins (GFPs) were examined in numerous corals in the Caribbean Sea, the Egyptian Red Sea, the Indonesian Dampier Strait, and the Florida Keys. Below 2 m depth, solar induced NIR chlorophyll fluorescence can be imaged in daylight without external lighting, thus, it is much easier to do than visible fluorescence imaging done at night. The distributions of chlorophyll and GFPs are unique in every species examined, and while there are some tissues where both fluorophores are co-resident, often tissues are selectively enriched in only one of these fluorescent pigments. Although laboratory studies have clearly shown that GFPs can be photo-protective, their inability to prevent large scale bleaching events in situ may be due to their limited tissue distribution.

Near-infrared fluorescent probes for the detection of glutathione and their application in the fluorescence imaging of living cells and tumor-bearing mice

2018 ◽  
Vol 6 (17) ◽  
pp. 2541-2546 ◽  
Author(s):  
Dayoung Lee ◽  
Keunsoo Jeong ◽  
Xiao Luo ◽  
Gayoung Kim ◽  
Youjun Yang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Living Cells ◽  
Tumor Bearing

We present two new cyanine-based NIR fluorescent probes for imaging GSH in living cells and tumor-bearing mice.

scholarly journals Application of FTIR and Raman Spectroscopy to Characterisation of Bioactive Materials and Living Cells

2003 ◽  
Vol 17 (2-3) ◽  
pp. 275-288 ◽  
Author(s):  
I. Notingher ◽  
J. R. Jones ◽  
S. Verrier ◽  
I. Bisson ◽  
P. Embanga ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Near Infrared ◽  
Cell Damage ◽  
Living Cells ◽  
Biologically Active ◽  
Bioactive Materials ◽  
45S5 Bioglass ◽  
Ftir And Raman Spectroscopy

Both Fourier Transform Infrared (FTIR) and Raman spectroscopy have been applied to thein vitrocharacterisation of biomaterials, mainly surface reactions leading to the formation of a biologically active hydroxycarbonate apatite (HCA) layer on the sample surface when immersed in simulated body fluids (SBF). The HCA layer indicates the degree of bioactivity of the sample, because it leads to a strong bond between the biomaterial and living tissue. Reflection measurements using FTIR allow quick, non-destructive detection of the HCA layer for solid and powder samples. Due to the low Raman scattering efficiency and low absorption of water in the visible-near infrared region, Raman micro-spectroscopy was successfully used for thein situcharacterisation of 20 and 40µm diameter 45S5 Bioglass®fibres. Thein situcapabilities of the Raman micro-spectrometer have also been extended to the characterisation of living cells attached on bioinert silica and bioactive 45S5 Bioglass®and 58S substrates. Using a high power 785 nm laser, living cells in physiological conditions can be real-time sampled over long periods of time without inducing cell damage and with good signal strength. Cell death can be monitored because it proved to induce strong changes in the Raman signature in the spectral regions 1000–1150 cm–1and 1550–1650 cm–1.

Near-Infrared Two-Photon Fluorescent Chemodosimeter Based on Rhodamine-BODIPY for Mercury Ion Fluorescence Imaging in Living Cells

2017 ◽  
Vol 2 (31) ◽  
pp. 9970-9976 ◽  
Author(s):  
Baoxing Shen ◽  
Ying Qian ◽  
Zhengqing Qi ◽  
Changgui Lu ◽  
Yiping Cui
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Living Cells ◽  
Mercury Ion ◽  
Two Photon ◽  
Fluorescent Chemodosimeter

Nondestructive Structural Analysis of Photosynthetic Pigments in Living Rhodobacter Sphaeroides Mutants by Near-Infrared Fourier Transform Raman Spectroscopy

1992 ◽  
Vol 46 (3) ◽  
pp. 518-523 ◽  
Author(s):  
K. Okada ◽  
E. Nishizawa ◽  
Y. Fujimoto ◽  
Y. Koyama ◽  
S. Muraishi ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Rhodobacter Sphaeroides ◽  
Photosynthetic Pigments ◽  
Photosynthetic Bacteria ◽  
Near Infrared ◽  
Living Cells ◽  
Electronic Transitions ◽  
Fourier Transform Raman Spectroscopy ◽  
Fourier Transform Raman

The 1064-nm excited Fourier transform Raman spectra have been measured for two kinds of whole living cells of photosynthetic bacteria, Rhodobacter sphaeroides G1C and R26 mutants, to investigate in situ structures of photosynthetic pigments, bacteriochlorophyll- a (BChl- a) and neurosporene (the only carotenoid included in the G1C mutant). The 1064-nm excited spectra consist of contributions from both neurosporene and BChl- a in the light harvesting (LH) complexes (G1C) or from BChl- a alone in the LH complexes (R26). The pattern of the 1064-nm excited spectrum of BChl- a in the LH complexes, whose Raman bands are pre-resonance enhanced via its Qy band, is dramatically different from that of its 355-nm excited spectrum, whose Raman bands are resonance enhanced via the B bands; for example, a band at 1606 cm−1 due to a Ca=Cm stretching mode of BChl- a, which is the most intense in the 355-nm excited spectrum, is barely observed in the 1064-nm excited spectrum. The frequency of the above band indicates that BChl- a in the LH complexes is five-coordinate. Bands due to C=O stretching modes of the 9-keto and 2-acetyl groups of BChl- a appear clearly near 1665 and 1640 cm−1, respectively, in the 1064-nm excited spectra. The frequencies of these C=O stretching bands suggest that most of the 9-keto and 2-acetyl groups of BChl- a in the complexes are involved in intermolecular interaction with the proteins. Bands assignable to Ca=N and Ca=Cb stretching modes, which are, in general, very weak in the 355-nm excited spectra, appear strongly in the 1064-nm excited spectra, implying that their bond lengths are changed sizably in the electronic transitions corresponding to the Qy bands.

Construction of a novel ratiometric near-infrared fluorescent probe for SO2 derivatives and its application for biological imaging

2017 ◽  
Vol 9 (25) ◽  
pp. 3790-3794 ◽  
Author(s):  
Huiming Shang ◽  
Keyin Liu ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Living Cells ◽  
Biological Imaging ◽  
Ratiometric Fluorescent Probe

A novel near-infrared ratiometric fluorescent probe for detecting SO2 derivatives was developed and used for fluorescence imaging in living cells.

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