scholarly journals A Dual Fluorescence‐Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM – EPR Spectroscopy

2021 ◽  
Author(s):  
Pin Dong ◽  
Johannes Stellmacher ◽  
Lydia M. Bouchet ◽  
Marius Nieke ◽  
Amit Kumar ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Spin Label ◽  
Dual Fluorescence ◽  
Label Probe ◽  
Target Molecules

Intracellular viscosity of lymphocytes determined by a 15N spin label probe

1979 ◽  
Vol 36 (2) ◽  
pp. 207-214 ◽  
Author(s):  
W.R Hedrick ◽  
A Mathew ◽  
J.D Zimbrick ◽  
T.W Whaley
Keyword(s):  
Spin Label ◽  
Label Probe

The interaction of ligands with chemically modified phosphorylase b

1976 ◽  
Vol 54 (5) ◽  
pp. 494-499
Author(s):  
D. Brooks ◽  
S. J. W. Busby ◽  
J. R. Griffiths ◽  
G. K. Radda ◽  
O. Avramovic-Zikic
Keyword(s):  
Conformational Changes ◽  
Spin Label ◽  
Native Enzyme ◽  
Carboxyl Groups ◽  
Chemically Modified ◽  
Allosteric Effector ◽  
Spin Resonance ◽  
Label Probe ◽  
Glycine Ethyl Ester ◽  
Modified Enzyme

Phosphorylase b which had been inactivated with 5-diazo-1H-tetrazole was specifically labelled with 4-iodoacetamidosalicylic acid (a fluorescent probe) or with N-(1-oxyl-2,2,6,6,-tetramethyl-4-piperidinyl)iodoacetamide (a spin label probe) so that the binding of ligands and accompanying conformational changes could be determined by fluorescence or electron spin resonance changes, respectively. The allosteric effector, AMP, causes conformational changes similar to those caused in the native enzyme. The affinity of binding of phosphate or AMP to the inhibited protein is the same as for the unmodified protein. The heterotropic interactions between glucose-1-phosphate or glycogen and AMP are much less in the inactivated enzyme than in unmodified phosphorylase. Using a light scattering assay, it is shown that the modified enzyme binds to glycogen less strongly than the native protein.Phosphorylase b which had been inactivated by carbodiimide in the presence of glycine ethyl ester, resulting in the modification of one or more carboxyl groups, was labelled with the spin label probe described above. The modified enzyme has an affinity for AMP similar to that of the native enzyme. AMP binding to the modified enzyme is tightened by glycogen, weakened by glucose-6-phosphate and is unaffected by glucose- 1-phosphate.The actions of 5-diazo-1H-tetrazole and carbodiimide on phosphorylase are discussed in the light of the above observations.

Mapping the location of DATEM in multi-phase systems: Synthesis and characterization of spin-label probe analogues

2019 ◽  
Vol 275 ◽  
pp. 474-479 ◽  
Author(s):  
Sampson Anankanbil ◽  
Jens Henrik Mose ◽  
Bianca Pérez ◽  
Weiwei Cheng ◽  
Jacob Nedergaard Pedersen ◽  
...  
Keyword(s):  
Spin Label ◽  
Synthesis And Characterization ◽  
Label Probe ◽  
Phase Systems ◽  
Multi Phase

A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies

2018 ◽  
Vol 130 (5) ◽  
pp. 1380-1384 ◽  
Author(s):  
Ganesan Karthikeyan ◽  
Alessio Bonucci ◽  
Gilles Casano ◽  
Guillaume Gerbaud ◽  
Sébastien Abel ◽  
...  
Keyword(s):  
Structural Dynamics ◽  
Epr Spectroscopy ◽  
Spin Label ◽  
Protein Structural Dynamics

A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies

2018 ◽  
Vol 57 (5) ◽  
pp. 1366-1370 ◽  
Author(s):  
Ganesan Karthikeyan ◽  
Alessio Bonucci ◽  
Gilles Casano ◽  
Guillaume Gerbaud ◽  
Sébastien Abel ◽  
...  
Keyword(s):  
Structural Dynamics ◽  
Epr Spectroscopy ◽  
Spin Label ◽  
Protein Structural Dynamics

scholarly journals Site-Directed Spin-Label EPR Spectroscopy of M2 Protein in Lipid Bilayers of Different Curvature Generation Propensity

2017 ◽  
Vol 112 (3) ◽  
pp. 505a
Author(s):  
Douglas S. Arbuckle ◽  
Kathleen P. Howard ◽  
Tyler Alexander ◽  
Catherine Crouch
Keyword(s):  
Lipid Bilayers ◽  
Epr Spectroscopy ◽  
Spin Label ◽  
M2 Protein

scholarly journals A Spirocyclohexyl Nitroxide Amino Acid Spin Label for Pulsed EPR Spectroscopy Distance Measurements

2010 ◽  
Vol 16 (19) ◽  
pp. 5778-5782 ◽  
Author(s):  
Andrzej Rajca ◽  
Velavan Kathirvelu ◽  
Sandip K. Roy ◽  
Maren Pink ◽  
Suchada Rajca ◽  
...  
Keyword(s):  
Amino Acid ◽  
Epr Spectroscopy ◽  
Spin Label ◽  
Distance Measurements ◽  
Pulsed Epr ◽  
Nitroxide Amino Acid
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