scholarly journals High‐Throughput Label‐Free Enzymatic Assays Using Desorption Electrospray‐Ionization Mass Spectrometry

2020 ◽  
Vol 132 (46) ◽  
pp. 20639-20644
Author(s):  
Nicolás M. Morato ◽  
Dylan T. Holden ◽  
R. Graham Cooks
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
High Throughput ◽  
Electrospray Ionization Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Label Free ◽  
Enzymatic Assays

High-throughput screening of organic reactions in microdroplets using desorption electrospray ionization mass spectrometry (DESI-MS): hardware and software implementation

2020 ◽  
Vol 12 (28) ◽  
pp. 3654-3669
Author(s):  
Tiago Jose P. Sobreira ◽  
Larisa Avramova ◽  
Botond Szilagyi ◽  
David L. Logsdon ◽  
Bradley P. Loren ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
High Throughput ◽  
Electrospray Ionization Mass Spectrometry ◽  
High Throughput Screening ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Desi Ms ◽  
Novel Method

Implementation of a novel method for high-throughput screening of reactions in microdroplets. The reaction and analysis steps are performed simultaneously using desorption electrospray ionization mass spectrometry (DESI-MS) at a rate of up to 1 reaction mixture per second.

scholarly journals A label-free Sirtuin 1 assay based on droplet-electrospray ionization mass spectrometry

2016 ◽  
Vol 8 (17) ◽  
pp. 3458-3465 ◽  
Author(s):  
Shuwen Sun ◽  
Benjamin C. Buer ◽  
E. Neil G. Marsh ◽  
Robert T. Kennedy
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
High Throughput ◽  
Electrospray Ionization Mass Spectrometry ◽  
Sirtuin 1 ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Label Free ◽  
Ion Suppression ◽  
Free Ion

A reliable, label-free, ion suppression-free Sirtuin 1 assay has been developed. By interfacing multi-well plates to electrospray ionization mass spectrometry by oil-segmented droplets, the assay can be applied for high throughput Sirtuin 1 modulator screening.

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