Efficient Gene Transfection through Inhibition of β-Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles

2017 ◽  
Vol 129 (28) ◽  
pp. 8195-8200 ◽  
Author(s):  
Poulami Jana ◽  
Krishnananda Samanta ◽  
Sandra Bäcker ◽  
Elio Zellermann ◽  
Shirley Knauer ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gene Transfection ◽  
Fiber Formation ◽  
Efficient Gene ◽  
Amphiphilic Peptide ◽  
Amyloid Fiber Formation ◽  
Β Sheet

Efficient Gene Transfection through Inhibition of β-Sheet (Amyloid Fiber) Formation of a Short Amphiphilic Peptide by Gold Nanoparticles

2017 ◽  
Vol 56 (28) ◽  
pp. 8083-8088 ◽  
Author(s):  
Poulami Jana ◽  
Krishnananda Samanta ◽  
Sandra Bäcker ◽  
Elio Zellermann ◽  
Shirley Knauer ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gene Transfection ◽  
Fiber Formation ◽  
Efficient Gene ◽  
Amphiphilic Peptide ◽  
Amyloid Fiber Formation ◽  
Β Sheet

scholarly journals Chitosan-conjugated lipid microbubble combined with ultrasound for efficient gene transfection

2018 ◽  
Vol 32 (4) ◽  
pp. 982-987 ◽  
Author(s):  
Hui Zhang ◽  
Jiawei Tu ◽  
Yangying Liao ◽  
Kuan Cai ◽  
Yue Li ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Efficient Gene

scholarly journals Ionic liquids with thioether motifs as synthetic cationic lipids for gene delivery

2017 ◽  
Vol 53 (59) ◽  
pp. 8328-8331 ◽  
Author(s):  
Jamie C. Gaitor ◽  
Lauren M. Paul ◽  
Melissa M. Reardon ◽  
Taha Hmissa ◽  
Samuel Minkowicz ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Gene Delivery ◽  
Click Chemistry ◽  
Gene Transfection ◽  
Cationic Lipids ◽  
Efficient Gene

Novel lipidic ionic liquids with imidazolium headgroups (red), thioether linkers (black), and two hydrophobic tails (blue) as efficient gene transfection vectors, synthesized via thiol–yne click chemistry.

Functionalized Scaffold for in Situ Efficient Gene Transfection of Mesenchymal Stem Cells Spheroids toward Chondrogenesis

2018 ◽  
Vol 10 (40) ◽  
pp. 33993-34004 ◽  
Author(s):  
Kunxi Zhang ◽  
Haowei Fang ◽  
Yechi Qin ◽  
Lili Zhang ◽  
Jingbo Yin
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Transfection ◽  
Efficient Gene

Time evolution of β-lactoglobulin corona formation on gold nanoparticles and impact on allergy

2020 ◽  
Author(s):  
Xiaoning Zhang ◽  
Meifeng Li ◽  
Yuanping Lv ◽  
Xiaoling Sun ◽  
Yao Han ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Secondary Structure ◽  
Time Evolution ◽  
Electrostatic Force ◽  
Protein Corona ◽  
Raw Milk ◽  
Covalent Bonds ◽  
Corona Formation ◽  
Β Lactoglobulin ◽  
Β Sheet

Abstract Gold nanoparticles (AuNPs) are modified immediately by the adsorption of β-lactoglobulin (βlg) when designed as colorimetric probe in raw milk, leading to the formation of a protein corona. This adsorption results mainly from a fast electrostatic force and a slow formation of Au-S covalent bonds, which is a precondition for the use of AuNPs in biodetection. The proteins corona influences the structure and bioactivity of adsorbed protein, such as the allergy. In this study, the mechanism of βlg adsorbed on AuNPs was investigated in terms of stoichiometry, binding affinity (Ka), time evolution of Au-S bond, and general secondary structure changes to address the desensitization of AuNPs. The results show that about 3,600 βlg are adsorbed on a single AuNPs, and the Ka is 2.9 ± 0.7 × 10 6 M -1 . The formation of Au-S bonds takes about 9 h, which is the time needed for complete changes in secondary structure and the IgE combining capacity. The structure of allergenic epitopes assigned to β-sheet was destroyed by the formation of Au-S bond, then induced to the decrease allergy. Furthermore, Fourier transform infrared spectroscopy confirmed a decrease in β-sheet contents after conjugated with AuNPs.

A PEG-b-poly(disulfide-l-lysine) based redox-responsive cationic polymer for efficient gene transfection

2019 ◽  
Vol 7 (11) ◽  
pp. 1893-1905 ◽  
Author(s):  
Ihsan Ullah ◽  
Jing Zhao ◽  
Shah Rukh ◽  
Khan Muhammad ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Cationic Polymer ◽  
Endosomal Escape ◽  
Gene Vector ◽  
Efficient Gene ◽  
Redox Responsive ◽  
Responsive Gene

A redox responsive gene vector efficiently delivered pDNA with a good internalization tendency, efficient endosomal escape and high nucleus internalization.

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