scholarly journals Blocking Deprotonation with Retention of Aromaticity in a Plant ent -Copalyl Diphosphate Synthase Leads to Product Rearrangement

2015 ◽  
Vol 128 (2) ◽  
pp. 644-648 ◽  
Author(s):  
Kevin C. Potter ◽  
Jiachen Zi ◽  
Young J. Hong ◽  
Samuel Schulte ◽  
Brandi Malchow ◽  
...  
Keyword(s):  
Copalyl Diphosphate Synthase

scholarly journals Structure and mechanism of the diterpene cyclase ent-copalyl diphosphate synthase

2011 ◽  
Vol 7 (7) ◽  
pp. 431-433 ◽  
Author(s):  
Mustafa Köksal ◽  
Huayou Hu ◽  
Robert M Coates ◽  
Reuben J Peters ◽  
David W Christianson
Keyword(s):  
Diterpene Cyclase ◽  
Copalyl Diphosphate Synthase

scholarly journals Computer-Aided Saturation Mutagenesis of Arabidopsis thaliana Ent-Copalyl Diphosphate Synthase

2019 ◽  
Vol 12 (1) ◽  
pp. 32-43 ◽  
Author(s):  
Piotr Szymczyk ◽  
Grażyna Szymańska ◽  
Anna Lipert ◽  
Izabela Weremczuk-Jeżyna ◽  
Ewa Kochan
Keyword(s):  
Arabidopsis Thaliana ◽  
Saturation Mutagenesis ◽  
Computer Aided ◽  
Copalyl Diphosphate Synthase

scholarly journals Isolation and characterization of a copalyl diphosphate synthase gene promoter from Salvia miltiorrhiza

2016 ◽  
Vol 85 (3) ◽  
Author(s):  
Piotr Szymczyk ◽  
Ewa Skała ◽  
Renata Grąbkowska ◽  
Agnieszka Jeleń ◽  
Marta Żebrowska ◽  
...  
Keyword(s):  
Binding Sites ◽  
Promoter Activity ◽  
Tandem Repeats ◽  
Salvia Miltiorrhiza ◽  
Pcr Analysis ◽  
Biotic And Abiotic Stress ◽  
Rt Pcr ◽  
Isolation And Characterization ◽  
Protein Encoding ◽  
Copalyl Diphosphate Synthase

<p>The promoter, 5' UTR, and 34-nt 5' fragments of protein encoding region of the <em>Salvia miltiorrhiza</em> copalyl diphosphate synthase gene were cloned and characterized. No tandem repeats, miRNA binding sites, or CpNpG islands were observed in the promoter, 5' UTR, or protein encoding fragments. The entire isolated promoter and 5' UTR is 2235 bp long and contains repetitions of many <em>cis</em>-active elements, recognized by homologous transcription factors, found in <em>Arabidopsis thaliana</em> and other plant species. A pyrimidine-rich fragment with only 6 non-pyrimidine bases was localized in the 33-nt stretch from nt 2185 to 2217 in the 5' UTR. The observed <em>cis</em>-active sequences are potential binding sites for <em>trans</em>-factors that could regulate spatio-temporal <em>CPS</em> gene expression in response to biotic and abiotic stress conditions. Obtained results are initially verified by in silico and co-expression studies based on <em>A. thaliana</em> microarray data.</p><p>The quantitative RT-PCR analysis confirmed that the entire 2269-bp copalyl diphosphate synthase gene fragment has the promoter activity.</p><p>Quantitative RT-PCR analysis was used to study changes in <em>CPS</em> promoter activity occurring in response to the application of four selected biotic and abiotic regulatory factors; auxin, gibberellin, salicylic acid, and high-salt concentration.</p>

scholarly journals Presence of Copalyl Diphosphate Synthase Gene in an Actinomycete Possessing the Mevalonate Pathway

2004 ◽  
Vol 57 (11) ◽  
pp. 739-747 ◽  
Author(s):  
TAKASHI KAWASAKI ◽  
TOMOHISA KUZUYAMA ◽  
YUKO KUWAMORI ◽  
NOBUYASU MATSUURA ◽  
NOBUYA ITOH ◽  
...  
Keyword(s):  
Mevalonate Pathway ◽  
Copalyl Diphosphate Synthase
Sign in / Sign up

Export Citation Format

Share Document