scholarly journals Stereochemical Assignment of the Protein-Protein Interaction Inhibitor JBIR-22 by Total Synthesis

2015 ◽  
Vol 127 (13) ◽  
pp. 4118-4122 ◽  
Author(s):  
Alan R. Healy ◽  
Miho Izumikawa ◽  
Alexandra M. Z. Slawin ◽  
Kazuo Shin-ya ◽  
Nicholas J. Westwood
Keyword(s):  
Total Synthesis ◽  
Protein Interaction ◽  
Stereochemical Assignment ◽  
Protein Protein Interaction

scholarly journals Stereochemical Assignment of the Protein-Protein Interaction Inhibitor JBIR-22 by Total Synthesis

2015 ◽  
Vol 54 (13) ◽  
pp. 4046-4050 ◽  
Author(s):  
Alan R. Healy ◽  
Miho Izumikawa ◽  
Alexandra M. Z. Slawin ◽  
Kazuo Shin-ya ◽  
Nicholas J. Westwood
Keyword(s):  
Total Synthesis ◽  
Protein Interaction ◽  
Stereochemical Assignment ◽  
Protein Protein Interaction

Total synthesis and characterization of thielocin B1 as a protein–protein interaction inhibitor of PAC3 homodimer

2014 ◽  
Vol 5 (5) ◽  
pp. 1860-1868 ◽  
Author(s):  
Takayuki Doi ◽  
Masahito Yoshida ◽  
Kosuke Ohsawa ◽  
Kazuo Shin-ya ◽  
Motoki Takagi ◽  
...  
Keyword(s):  
Total Synthesis ◽  
Protein Interaction ◽  
Synthesis And Characterization ◽  
Protein Protein Interaction

We have characterized the inhibition of the protein–protein interaction of the homodimer of proteasome assembling chaperone (PAC) 3 with thielocin B1.

An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Yu-Miao Zhang ◽  
Jun Wang ◽  
Tao Wu
Keyword(s):  
Subcellular Localization ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Epidermal Cells ◽  
Cyclin Dependent Kinase ◽  
Protein Subcellular Localization ◽  
Protein Protein Interactions ◽  
Efficient System ◽  
Protein Protein Interaction ◽  
Onion Epidermal Cells

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.

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