Noninvasive Saliva Collection for DNA Analyses From Free-Ranging Tibetan Macaques (Macaca thibetana)

2012 ◽  
Vol 74 (11) ◽  
pp. 1064-1070 ◽  
Author(s):  
N. D. SIMONS ◽  
J. G. LORENZ ◽  
L. K. SHEERAN ◽  
J. H. LI ◽  
D. P. XIA ◽  
...  
2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Binghua Sun ◽  
Xi Wang ◽  
Sofi Bernstein ◽  
Michael A. Huffman ◽  
Dong-Po Xia ◽  
...  

2018 ◽  
Vol 80 (7) ◽  
pp. e22880 ◽  
Author(s):  
Binghua Sun ◽  
Zhiyuan Gu ◽  
Xi Wang ◽  
Michael A. Huffman ◽  
Paul A. Garber ◽  
...  

2010 ◽  
Vol 41 (2) ◽  
pp. 201-209 ◽  
Author(s):  
Tierra Smiley ◽  
Lucy Spelman ◽  
Magdalena Lukasik-Braum ◽  
Jean Mukherjee ◽  
Gretchen Kaufman ◽  
...  

Primates ◽  
2011 ◽  
Vol 53 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Dongpo Xia ◽  
Jinhua Li ◽  
Megan D. Matheson ◽  
Lixing Sun ◽  
Binghua Sun ◽  
...  

1992 ◽  
Vol 72 (2) ◽  
pp. 245-252 ◽  
Author(s):  
T. Sahlu ◽  
N. Jacquemet ◽  
H. Carneiro ◽  
S. P. Hart ◽  
P. D. Klein

The use and limitations of 13C stable isotopes as tracers in animal experimentation was investigated using 13C-labeled bicarbonate to estimate CO2 production and energetic requirement of free-ranging goats. Goats were fed wheat hay (Triticum aestivum; C3) or Old World Bluestem hay (Bothriochloa caucasica; C4; OWBS) ad libitum for 21 d. Parotid salivary ducts were cannulated for saliva collection. Isotopic bicarbonate (13C) was infused at a constant rate into the peritoneal cavity. In exp. 1, saliva samples were obtained at successive 2-h periods for 20 h to establish the time to achieve equilibrium. The half-life of bicarbonate was 2.9 h for animals consuming the wheat diet and 4.1 h (P < 0.06) for animals consuming OWBS diet. The time required to reach equilibrium was 12 h for animals on the wheat diet and 16 h for animals on OWBS diet. The error in the technique arising from animals consuming C3 vs. C4 species (which have different endogenous 13C contents) was studied in exp. 2. If species composition (C3 vs. C4) of the diet remains constant within 10%, the error is negligible. In exp. 3, estimation of CO2 production from continuous saliva samples had a lower coefficient of variation (6.3 vs. 10.0%) than measurements based on spot serum samples. Energy expenditure of goats in stalls consuming hay ad libitum was similar to that of ad-libitum-fed sheep at similar body weights. The 13C methodology appears to be a promising method for measuring energy expenditure of grazing animals. Key words: Energy requirement, stable isotopes, carbon dioxide, sodium bicarbonate


2014 ◽  
Vol 60 (3) ◽  
pp. 362-372 ◽  
Author(s):  
Alexander J. Pritchard ◽  
Lori K. Sheeran ◽  
Kara I. Gabriel ◽  
Jin-Hua Li ◽  
Ronald S. Wagner

Abstract To further the potential for applied personality studies, we present a methodology for assessing personality in nonhuman animals without a priori assumptions, using behavioral measures to discriminate personality survey results. Our study group consisted of 12 free-ranging, provisioned, adult Tibetan macaques Macaca thibetana at the Valley of the Wild Monkeys, China. We asked familiar Chinese park guards and scientists to rate each of the 12 macaques using 27-item personality surveys. We also recorded behavioral observations (&gt; 100 hrs) from August–September, 2012. The personality surveys showed reliability in 22 of the items that were then utilized in a principal component analysis that revealed five components: Insecurity, Reactivity, Boldness, Sociability, and Leadership. Prior personality research on Macaca show comparable components. In order to determine which behaviors would best predict those five personality components, we conducted discriminant analyses using behavioral measures as predictors. We found that behavioral measures of avoidance, lunging, fear-grinning, self-directed behaviors, touching, proximity and chasing could significantly predict personality component scores in certain situations. Finally, we analyzed the effects of situation (provisioning and tourists) and found situation influenced proximity and rates of avoidance and self-directed behaviors. Wider implementation of this methodology may permit long-term analysis of personality using behavioral proxies for established personality traits, in particular on research investigating the effects of tourism and provisioning on personality.


2017 ◽  
Vol 63 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Dong-Po Xia ◽  
Xi Wang ◽  
Qi-Xin Zhang ◽  
Bing-Hua Sun ◽  
Lixing Sun ◽  
...  

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
M Monschein ◽  
T Holzer ◽  
V Wolkinger ◽  
H Heuberger ◽  
R Seidenberger ◽  
...  

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