scholarly journals Development of olfactory nerve glia defined by a monoclonal antibody specific for schwann cells

1992 ◽  
Vol 194 (3) ◽  
pp. 231-238 ◽  
Author(s):  
Robert B. Norgren ◽  
Nancy Ratner ◽  
Robert Brackenbury
Keyword(s):  
Monoclonal Antibody ◽  
Schwann Cells ◽  
Olfactory Nerve

Monoclonal antibody stains oligodendrocytes and Schwann cells in zebrafish ( Danio rerio )

2000 ◽  
Vol 201 (5) ◽  
pp. 399-406 ◽  
Author(s):  
Kaori Tomizawa ◽  
Yasushi Inoue ◽  
Shima Doi ◽  
H. Nakayasu
Keyword(s):  
Monoclonal Antibody ◽  
Schwann Cells ◽  
Danio Rerio

Expression of Schwann cell markers by mammalian neural crest cells in vitro

Development ◽  
1989 ◽  
Vol 105 (2) ◽  
pp. 251-262 ◽  
Author(s):  
L.C. Smith-Thomas ◽  
J.W. Fawcett
Keyword(s):  
Monoclonal Antibody ◽  
Schwann Cell ◽  
Embryonic Development ◽  
Neural Crest ◽  
Schwann Cells ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Similar Proportion ◽  
Cell Phenotype ◽  
Cell Markers

During embryonic development, neural crest cells differentiate into a wide variety of cell types including Schwann cells of the peripheral nervous system. In order to establish when neural crest cells first start to express a Schwann cell phenotype immunocytochemical techniques were used to examine rat premigratory neural crest cell cultures for the presence of Schwann cell markers. Cultures were fixed for immunocytochemistry after culture periods ranging from 1 to 24 days. Neural crest cells were identified by their morphology and any neural tube cells remaining in the cultures were identified by their epithelial morphology and immunocytochemically. As early as 1 to 2 days in culture, approximately one third of the neural crest cells stained with m217c, a monoclonal antibody that appears to recognize the same antigen as rat neural antigen-1 (RAN-1). A similar proportion of cells were immunoreactive in cultures stained with 192-IgG, a monoclonal antibody that recognizes the rat nerve growth factor receptor. The number of immunoreactive cells increased with time in culture. After 16 days in culture, nests of cells, many of which had a bipolar morphology, were present in the area previously occupied by neural crest cells. The cells in the nests were often associated with neurons and were immunoreactive for m217c, 192-IgG and antibody to S-100 protein and laminin, indicating that the cells were Schwann cells. At all culture periods examined, neural crest cells did not express glial fibrillary acidic protein. These results demonstrate that cultured premigratory neural crest cells express early Schwann cell markers and that some of these cells differentiate into Schwann cells. These observations suggest that some neural crest cells in vivo may be committed to forming Schwann cells and will do so provided that they then proceed to encounter the correct environmental cues during embryonic development.

scholarly journals Development of oligodendrocytes and Schwann cells studied with a monoclonal antibody against galactocerebroside.

1982 ◽  
Vol 79 (8) ◽  
pp. 2709-2713 ◽  
Author(s):  
B. Ranscht ◽  
P. A. Clapshaw ◽  
J. Price ◽  
M. Noble ◽  
W. Seifert
Keyword(s):  
Monoclonal Antibody ◽  
Schwann Cells

04 and A007-sulfatide antibodies bind to embryonic Schwann cells prior to the appearance of galactocerebroside; regulation of the antigen by axon-Schwann cell signals and cyclic AMP

Development ◽  
1990 ◽  
Vol 109 (1) ◽  
pp. 105-116 ◽  
Author(s):  
R. Mirsky ◽  
C. Dubois ◽  
L. Morgan ◽  
K.R. Jessen
Keyword(s):  
Monoclonal Antibody ◽  
Sciatic Nerve ◽  
Schwann Cell ◽  
Schwann Cells ◽  
Prenatal Development ◽  
Intracellular Camp ◽  
Distal Stump ◽  
Surface Binding ◽  
Adult Rat ◽  
Schwann Cell Development

In the rat sciatic nerve, the relationship between Schwann cells, axons, the extracellular matrix and perineurial sheath cells undergoes extensive modification between embryo day 15 and the onset of myelination during the first postnatal day. Little is known about molecular changes in Schwann cells in this important prenatal period. In the present paper, we use immunofluorescence to study the prenatal development and postnatal regulation of the antigen(s) recognized by the 04 monoclonal antibody and a well-characterized rat monoclonal antibody to sulfatide, A007. We show that, in a series of immunochemical tests, the 04 antibody recognizes only sulfatide in neonatal and adult rat nerves. Both antibodies first bind to Schwann cells in the sciatic nerve at embryo day 16–17, and all Schwann cells bind both antibodies at birth. In the adult nerve, both nonmyelin-forming and myelin-forming cells are labelled with the antibodies. Schwann cells dissociated from embryo day 15 nerves and cultured in the absence of axons develop neither 04 nor A007 binding on schedule, and 04-positive and A007-positive Schwann cells from postnatal nerves lose the ability to bind these antibodies during the first few days in culture. Schwann cells in the distal stump of transected nerves also sharply down-regulate cell surface binding of 04. High numbers of 04-positive or A007-positive Schwann cells reappear in cultures treated with agents that mimic or elevate intracellular cAMP. We conclude that two anti-sulfatide antibodies 04 and A007, recognize an antigen, probably sulfatide, that appears very early in Schwann cell development (one to two days prior to galactocerebroside) but is nevertheless subject to upregulation by axonal contact or elevation of intracellular cAMP.

scholarly journals ELECTRON MICROSCOPIC OBSERVATIONS OF THE OLFACTORY MUCOSA AND OLFACTORY NERVE

1957 ◽  
Vol 3 (6) ◽  
pp. 839-850 ◽  
Author(s):  
A. J. de Lorenzo
Keyword(s):  
Schwann Cells ◽  
Bipolar Cell ◽  
Receptor Cell ◽  
Supporting Cell ◽  
Olfactory Nerve ◽  
Olfactory Mucosa ◽  
Electron Microscopic ◽  
Olfactory Neurons ◽  
Distal Process ◽  
Electrophysiological Studies

The olfactory receptor cell is characterized by a distal process (the dendrite) which terminates in the olfactory passage as the olfactory rod. The olfactory rod is provided with numerous cilia which are similar in structure to those seen in other tissues. The central processes of the bipolar cell constitute the fila olfactoria. The cytoplasmic organelles of the sustentacular cell are concentrated at the apical and basal ends of the cell with a paucity of cytoplasmic elements in the region of the nucleus. The plasma membrane of the supporting cell forms a mesaxon for both the dendrite and axon of the bipolar cell. Terminal bars are present in the epithelial cells. The axons constituting the fila olfactoria form fascicles which are ensheathed by mesaxons of adjacent Schwann cells. Thus the olfactory neurons are ensheathed throughout their course by the membranes of sustentacular and Schwann cells. Observations of the olfactory mucosa with the electron microscope are discussed with respect to recent electrophysiological studies.

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