The corpus luteum of the guinea pig. Fine structure at the time of maximum progesterone secretion and during regression

1977 ◽  
Vol 150 (4) ◽  
pp. 565-603 ◽  
Author(s):  
Laurie G. Paavola
Keyword(s):  
Fine Structure ◽  
Guinea Pig ◽  
Corpus Luteum ◽  
Progesterone Secretion

EFFECT OF INTRA-UTERINE FOREIGN BODIES AND OF PROSTAGLANDIN ADMINISTRATION ON PROGESTERONE SECRETION DURING THE OESTROUS CYCLE OF THE GUINEA-PIG

1976 ◽  
Vol 70 (1) ◽  
pp. 39-45 ◽  
Author(s):  
F. R. BLATCHLEY ◽  
B. T. DONOVAN
Keyword(s):  
Guinea Pig ◽  
Corpus Luteum ◽  
Foreign Bodies ◽  
Glass Beads ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Blood Samples ◽  
Progesterone Secretion ◽  
Prostaglandin F

SUMMARY The response of the guinea-pig corpus luteum to the luteolytic influence of glass beads placed in the uterus, or to prostaglandin administration, was followed by assay of the progesterone content of blood samples collected daily. Following the introduction of glass beads into the uterus early in the cycle, the secretion of progesterone was curtailed. Treatment with prostaglandin F2α over days 4–6 or 6–8 of the cycle temporarily depressed progesterone release without shortening the life of the corpora lutea. When the drug was administered over days 8–10, 10–12 or 12–14 the depression in progesterone was not followed by any recovery. These observations indicate that the response of the corpora lutea to a luteolytic influence changes during the oestrous cycle.

scholarly journals The corpus luteum of the guinea pig. II. Cytochemical studies on the Golgi complex, GERL, and lysosomes in luteal cells during maximal progesterone secretion.

1978 ◽  
Vol 79 (1) ◽  
pp. 45-58 ◽  
Author(s):  
L G Paavola
Keyword(s):  
Guinea Pig ◽  
Corpus Luteum ◽  
Golgi Complex ◽  
Cell Types ◽  
Base Line ◽  
Vascular Perfusion ◽  
Luteal Cells ◽  
Progesterone Secretion ◽  
Low Levels ◽  
Lysosomal Proteins

This study characterizes the cytochemical properties of the Golgi complex, the structure which corresponds to Golgi complex-endoplasmic reticulum-lysosomes (GERL), and the granule population in luteal cells of guinea pigs at the time of maximum progesterone secretion, in material fixed by vascular perfusion, a method particularly suited for preserving both fine structure and enzyme activity. The distribution of several marker enzymes was determined by electron microscope cytochemistry. Acid phosphatase (ACPase) and arylsulfatase were used to identify structures containing lysosomal proteins. To resolve specific problems, additional cytochemical markers were employed: localization of thiamine pyrophosphatase (TPPase) (in the Golgi complex) and alkaline phosphatase (ALPase) (a plasma membrane marker), and prolonged osmication (a generally accepted method of marking the outer cisterna of the Golgi complex). The results demonstrate that at the time of peak steroid secretion the Golgi complex in luteal cells, in marked contrast to that of most other cell types, typically displays intense ACPase activity in all of its cisternae. Similarly, all Golgi cisternae stain after prolonged osmication and may show TPPase activity. On the other hand, GERL in luteal cells of this age, unlike that in most cells, commonly shows low levels of, or lacks, ACPase activity. However, GERL resembles that of other cell types in being TPPase-negative and in being unstained by treatment with aqueous OsO4. GERL and some Golgi cisternae are reactive for ALPase. The granule population in luteal cells of this stage consists of lysosomes, multivesicular bodies, electrontransparent vacuoles, and microperoxisome-like bodies. These results form a base line with which luteolytic changes described in the companion study (Paavola, L.G. 1978. The corpus luteum of the guinea pig. III. Cytochemical studies on the Golgi complex and GERL during normal postpartum regression of luteal cells, emphasizing the origin of lysosomes and autophagic vacuoles. J. Cell. Biol. 79:59--73.) can be compared.

The effects of prostaglandin F2? on the fine structure of the corpus luteum of the hysterectomized guinea pig

1975 ◽  
Vol 183 (2) ◽  
pp. 229-241 ◽  
Author(s):  
J. N. Bagwell ◽  
D. L. Davies ◽  
J. R. Ruby
Keyword(s):  
Fine Structure ◽  
Guinea Pig ◽  
Corpus Luteum

PROGESTERONE IN THE HUMAN PERIPHERAL BLOOD IN THE PREOVULATORY PERIOD OF THE MENSTRUAL CYCLE

1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Graafian Follicle ◽  
The Mean ◽  
Mean Concentration

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.

Local progesterone secretion by the marmoset corpus luteum after perfusion with regulating hormones

1987 ◽  
Vol 116 (3_Suppl) ◽  
pp. S111-S112 ◽  
Author(s):  
G.E. WEBLEY ◽  
J.P. HEARN ◽  
M.R. LUCK
Keyword(s):  
Corpus Luteum ◽  
Progesterone Secretion

The ultrastructure of the corpus luteum of the guinea-pig

1971 ◽  
Vol 115 (4) ◽  
pp. 473-493 ◽  
Author(s):  
Paula R. Crombie ◽  
Robert D. Burton ◽  
Nicola Ackland
Keyword(s):  
Guinea Pig ◽  
Corpus Luteum

Pulsatile secretion of progesterone from the human corpus luteum: poor correlation with bioactive LH pulses

1986 ◽  
Vol 111 (4) ◽  
pp. 553-557 ◽  
Author(s):  
Inese Z. Beitins ◽  
Maria L. Dufau
Keyword(s):  
Corpus Luteum ◽  
Temporal Relationship ◽  
Pulse Amplitude ◽  
Pulse Frequency ◽  
Biologically Active ◽  
Poor Correlation ◽  
Serum Progesterone ◽  
Progesterone Secretion ◽  
Wide Range ◽  
Human Corpus Luteum

Abstract. Having previously established that biologically active luteinizing hormone (LH) is secreted in episodic pulsations that vary in relation to the menstrual cycle, we investigated the possibility that a temporal relationship could exist between the bioactive LH pulses and progesterone secretion from the late corpus luteum. In 4 young women blood was withdrawn every 15 min for 8 h. Serum progesterone concentrations fluctuated at a mean frequency of 0.9 h with a wide range of amplitudes (13.8 to 1.7 ng/ml). Serum bioactive LH pulse frequency in contrast was 0.25 pulses/h in all subjects. The pulse amplitude was 18.2 to 12.4 mIU/ml (2nd IRP-hMG). These data reveal that within the 8 h-period studied, progesterone secretory pulses occurred four times more frequently as those for bioactive LH. Therefore it is unlikely that a temporal relationship exists between individual bioactive LH and pulses of progesterone secreted by the late corpus luteum.

Fine Structure of Guinea Pig Vestibular Kinocilium

1989 ◽  
Vol 108 (1-2) ◽  
pp. 26-30 ◽  
Author(s):  
Toshihiko Kikuchi ◽  
Tomonori Takasaka ◽  
Akira Tonosaki ◽  
Hiroshi Watanabe
Keyword(s):  
Fine Structure ◽  
Guinea Pig
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