scholarly journals Virus Disinfection: Virus Disinfection from Environmental Water Sources Using Living Engineered Biofilm Materials (Adv. Sci. 14/2020)

2020 ◽  
Vol 7 (14) ◽  
pp. 2070076
Author(s):  
Jiahua Pu ◽  
Yi Liu ◽  
Jicong Zhang ◽  
Bolin An ◽  
Yingfeng Li ◽  
...  
2019 ◽  
Vol 2 (3) ◽  
pp. 55 ◽  
Author(s):  
Brienna L. Anderson-Coughlin ◽  
Kalmia E. Kniel

The variability of environmental water samples impacts the allowance of one method to be universally ideal for all water types and volumes. Surface and reclaimed waters can be used for crop irrigation and may be referred to as non-traditional irrigation waters as these water types may be associated with a higher risk of microbial contamination compared to groundwater. These waters are typically more microbially and chemically complex than groundwater and have a higher risk of viral contamination. To detect viruses in these water types, an infinite number of variations can be made to traditional recovery methods. This protocol was developed based on a commonly used virus adsorption and elution (VIRADEL) method. Additional steps were included to simplify and efficiently reduce particulates in the viral concentrate and remove DNA from eluted nucleic acids prior to detection. Method alterations allow for volumes up to 40 liters to be processed with consistent recovery of enteric viruses including Aichi virus, hepatitis A virus, and noroviruses belonging to genogroups GI and GII. No inhibition was observed among either surface or reclaimed water samples. This protocol could be utilized in the monitoring of a wide array of irrigation water sources throughout irrigation processes.


The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1195-1201 ◽  
Author(s):  
Amy A. Bowyer ◽  
Clara Shen ◽  
Elizabeth J. New

A fluorescent sensing array based on analogues of Calcein Blue is able to classify toxic heavy metals in water.


2013 ◽  
Vol 36 (4) ◽  
pp. 198-203 ◽  
Author(s):  
Semra Ozcelik ◽  
Kubra Acikalin Coskun ◽  
Onder Yunlu ◽  
Ahmet Alim ◽  
Erdogan Malatyali

2020 ◽  
Vol 7 (14) ◽  
pp. 1903558 ◽  
Author(s):  
Jiahua Pu ◽  
Yi Liu ◽  
Jicong Zhang ◽  
Bolin An ◽  
Yingfeng Li ◽  
...  

2016 ◽  
Vol 10 (37) ◽  
pp. 1576-1580 ◽  
Author(s):  
B. Karkari Akosua ◽  
Obiri-Danso Kwasi ◽  
H. Frimpong Enoch ◽  
A. Krogfelt Karen

2015 ◽  
Vol 29 ◽  
pp. 35-41 ◽  
Author(s):  
Jingyu Guo ◽  
Ting Liang ◽  
Chaohui Hu ◽  
Ruichen Lv ◽  
Xianwei Yang ◽  
...  

Water ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 544 ◽  
Author(s):  
Pedro Teixeira ◽  
Sílvia Costa ◽  
Bárbara Brown ◽  
Susana Silva ◽  
Raquel Rodrigues ◽  
...  

Current regulations and legislation require critical revision to determine safety for alternative water sources and water reuse as part of the solution to global water crisis. In order to fulfill those demands, Lisbon municipality decided to start water reuse as part of a sustainable hydric resources management, and there was a need to confirm safety and safeguard for public health for its use in this context. For this purpose, a study was designed that included a total of 88 samples collected from drinking, superficial, underground water, and wastewater at three different treatment stages. Quantitative Polimerase Chain Reaction (PCR) detection (qPCR) of enteric viruses Norovirus (NoV) genogroups I (GI) and II (GII) and Hepatitis A (HepA) was performed, and also FIB (E. coli, enterococci and fecal coliforms) concentrations were assessed. HepA virus was only detected in one untreated influent sample, whereas NoV GI/ NoV GI were detected in untreated wastewater (100/100%), secondary treated effluent (47/73%), and tertiary treated effluent (33/20%). Our study proposes that NoV GI and GII should be further studied to provide the support that they may be suitable indicators for water quality monitoring targeting wastewater treatment efficiency, regardless of the level of treatment.


2020 ◽  
Vol 41 (S1) ◽  
pp. s306-s306
Author(s):  
Yoojin Kim ◽  
Carmen Cortes-Ramos ◽  
Chad Douglas Nix ◽  
Lauren Ogden ◽  
Molly Hale ◽  
...  

Background: During a 2-month period at an academic medical system, 4 cases of pansusceptible P. aeruginosa (PsA) meningitis were identified among neuroscience intensive care unit (NSICU) patients with an external ventricular device (EVD). Methods: We reviewed microbiology data for the previous 2 years to determine background PsA rates and to identify additional cases of PsA meningitis. A case was defined as the isolation of PsA from a CSF specimen. We convened a multidisciplinary group of stakeholders to review medical records of case patients and to conduct a series of observational rounds. Scalp swab specimens were collected from NSICU patients to detect possible skin colonization. Pulsed-field gel electrophoresis (PFGE) analyses were performed on PsA isolates from the 4 case patients and 5 patients with PsA isolates from other body sites. Results: There was no hospital-wide increase in PsA incidence, and no patient without an EVD had PsA cultured from CSF. Infections occurred, on average, 10 days (range, 6–15 days) after EVD insertion. Cases were geographically dispersed in the NSICU and did not share common staff. None of the PsA isolates were genetically related and all scalp cultures were negative. Observations included multiple opportunities for contact with water sources: sinks in proximity to the head of the bed, storage of supplies next to sinks, reuse of bath basins, and use of dilute peroxide to clean surgical wounds. Multiuse shampoos, conditioners and lotions, not approved for hospital use, were found on the unit. Furthermore, 3 of 4 patients received cefazolin >24 hours after 6 of their 7 neurosurgeries for an average of 4.7 days (range, 0.8–4 days). Care practices were changed to mitigate contact between EVD sites and environmental water sources, and extended cefazolin surgical prophylaxis was discontinued. EVD practices were revised, and clinical teams had their competency confirmed. No additional cases have been identified in the 16 months following these interventions. Conclusions: This cluster of EVD infections was likely caused by patient care practices that resulted in independent introductions of PsA from multiple nonsterile or contaminated water sources. Antibiotic selection of PsA by extended use of cefazolin perioperative prophylaxis may have also contributed. EVD care practices should be designed to limit contact between and EVD insertion sites and nonsterile water sources or potentially contaminated care supplies. To substantiate performance improvement efforts and ensure interinstitutional comparability, a practical, standardized EVD-associated infection surveillance definition is needed.Funding: NoneDisclosures: None


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