scholarly journals Super‐Resolution Microscopy: Hypotonic Stress Induces Fast, Reversible Degradation of the Vimentin Cytoskeleton via Intracellular Calcium Release (Adv. Sci. 18/2019)

2019 ◽  
Vol 6 (18) ◽  
pp. 1970112
Author(s):  
Leiting Pan ◽  
Ping Zhang ◽  
Fen Hu ◽  
Rui Yan ◽  
Manni He ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Calcium Release ◽  
Super Resolution ◽  
Hypotonic Stress ◽  
Reversible Degradation ◽  
Intracellular Calcium Release ◽  
Super Resolution Microscopy

scholarly journals Hypotonic Stress Induces Fast, Reversible Degradation of the Vimentin Cytoskeleton via Intracellular Calcium Release

2019 ◽  
Vol 6 (18) ◽  
pp. 1900865 ◽  
Author(s):  
Leiting Pan ◽  
Ping Zhang ◽  
Fen Hu ◽  
Rui Yan ◽  
Manni He ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Calcium Release ◽  
Hypotonic Stress ◽  
Reversible Degradation ◽  
Intracellular Calcium Release

scholarly journals Hypotonic stress induces fast, reversible degradation of the vimentin cytoskeleton via intracellular calcium release

2019 ◽  
Author(s):  
Leiting Pan ◽  
Ping Zhang ◽  
Fen Hu ◽  
Rui Yan ◽  
Manni He ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Calcium Release ◽  
Degradation Mechanism ◽  
Super Resolution ◽  
Fibroblast Cell ◽  
Cell Ultrastructure ◽  
Cell Manipulation ◽  
Ultrastructural Changes ◽  
Hypotonic Stress ◽  
Intracellular Calcium Release

SUMMARYThe dynamic response of the cell to osmotic changes is critical to its physiology and has been widely exploited for cell manipulation. Using 3D-STORM super-resolution microscopy, here we examine the hypotonic stress-induced ultrastructural changes of the cytoskeleton of a common fibroblast cell type. Unexpectedly, we observe a fast, yet reversible dissolution of the vimentin intermediate filament system that precedes ultrastructural changes of the supposedly more dynamic actin and tubulin cytoskeletal systems, as well as changes in cell morphology. In combination with calcium imaging and biochemical analysis, we next show that the vimentin-specific fast cytoskeletal degradation under hypotonic stress is due to proteolysis by the calcium-dependent protease calpain. We find the process to be activated by the hypotonic stress-induced calcium release from intracellular stores, and so is efficiently suppressed by inhibiting any part of the IP3-Ca2+-calpain pathway we establish. Together, our findings highlight an unexpected, fast degradation mechanism for the vimentin cytoskeleton in response to the external stimuli, and point to the significant, yet previously overlooked physiological impacts of hypotonic stress-induced intracellular calcium release on cell ultrastructure and function.

scholarly journals Orientia tsutsugamushi Inhibits Apoptosis of Macrophages by Retarding Intracellular Calcium Release

2002 ◽  
Vol 70 (8) ◽  
pp. 4692-4696 ◽  
Author(s):  
Mee-Kyung Kim ◽  
Seung-Yong Seong ◽  
Ju-Young Seoh ◽  
Tae-Hee Han ◽  
Hyeon-Je Song ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Calcium Release ◽  
Cytosolic Calcium ◽  
Orientia Tsutsugamushi ◽  
Cytosolic Calcium Concentration ◽  
Infected Cells ◽  
Intracellular Calcium Release ◽  
Calcium Redistribution ◽  
In Cells

ABSTRACT Orientia tsutsugamushi shows both pro- and antiapoptotic activities in infected vertebrate cells. Apoptosis of THP-1 cells induced by beauvericin was inhibited by O. tsutsugamushi infection. Beauvericin-induced calcium redistribution was significantly reduced and retarded in cells infected with O. tsutsugamushi. Antiapoptotic activities of O. tsutsugamushi in infected cells are most probably due to inhibition of the increase in the cytosolic calcium concentration.

scholarly journals A Membrane Estrogen Receptor Mediates Intracellular Calcium Release in Astrocytes

Endocrinology ◽  
2004 ◽  
Vol 145 (8) ◽  
pp. 3788-3795 ◽  
Author(s):  
Victor V. Chaban ◽  
Alexander J. Lakhter ◽  
Paul Micevych
Keyword(s):  
Estrogen Receptor ◽  
Intracellular Calcium ◽  
Calcium Release ◽  
Intracellular Calcium Release

scholarly journals N-terminus oligomerization is conserved in intracellular calcium release channels

2014 ◽  
Vol 459 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Spyros Zissimopoulos ◽  
Jason Marsh ◽  
Laurence Stannard ◽  
Monika Seidel ◽  
F. Anthony Lai
Keyword(s):  
Structure Function ◽  
Intracellular Calcium ◽  
Calcium Release ◽  
Function Parameter ◽  
Calcium Release Channels ◽  
Cellular Processes ◽  
N Terminus ◽  
Intracellular Calcium Release ◽  
Ca2 Channels

Intracellular Ca2+ channels are of paramount importance for numerous cellular processes. In the present paper we report on a novel N-terminus intersubunit interaction, an essential structure–function parameter, which is conserved in both families of intracellular Ca2+ channels.

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