scholarly journals Osteoporotic Bone Recovery by a Highly Bone‐Inductive Calcium Phosphate Polymer‐Induced Liquid‐Precursor

2019 ◽  
Vol 6 (19) ◽  
pp. 1900683 ◽  
Author(s):  
Shasha Yao ◽  
Xianfeng Lin ◽  
Yifei Xu ◽  
Yangwu Chen ◽  
Pengcheng Qiu ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Osteoporotic Bone ◽  
Liquid Precursor

Functionalized Calcium Phosphate-based Nanoparticles for the Treatment of Osteoporosis

2005 ◽  
Vol 873 ◽  
Author(s):  
Balasundaram G ◽  
Sato M ◽  
Webster TJ
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Amorphous Calcium Phosphate ◽  
Bone Growth ◽  
Osteoporotic Bone ◽  
Amino Groups ◽  
Calcium Phosphate Nanoparticles ◽  
Bioactive Agents ◽  
Bone Growth Factor

AbstractIn an effort to decrease the number of problems associated with osteoporosis, the long-term goal of the present study is to design calcium phosphate-based nanoparticles that specifically attach to areas of low bone density and once attached, allow for the targeted release of bioactive agents that can quickly increase bone formation. Efforts are focused on nanoparticles of calcium phosphate-based materials since they are similar in size and chemistry to the major inorganic components of bone. As a first step in this research, the objective of the present study was to synthesize nanoparticles of crystalline hydroxyapatite (or HA) and amorphous calcium phosphate. Crystalline HA is stable under physiological fluids and, thus, will release embedded bioactive agents slowly. Alternatively, amorphous calcium phosphate is highly biodegradable and will, thus, release embedded bioactive agents quickly. A further objective of the present study was to functionalize such inorganic biodegradable materials with amino groups which would allow for the subsequent attachment of entities to direct such nanoparticles to osteoporotic bone and increased bone formation once attached. One promising approach to direct the nanoparticles to osteoporotic bone is to attach antibodies to pentosidine on the nanoparticles since pentosidine is present in higher amounts in osteoporotic compared to healthy bone. A promising approach to increase bone growth once nanoparticles attach to osteoporotic bone, is to embed nanoparticles with regions of the bone growth factor: bone morphogenic protein-2 (or BMP-2). Results of this study demonstrated the successful synthesis of both crystalline HA and amorphous calcium phosphate nanoparticles. Furthermore, results showed that these nanoparticles can be functionalized with versatile amino groups. In this manner, this study takes the first steps toward utilizing calcium phosphate based nanoparticles to reverse bone loss associated with osteoporosis.

scholarly journals Functionalized DNA nanostructures as scaffolds for guided mineralization

2019 ◽  
Vol 10 (45) ◽  
pp. 10537-10542 ◽  
Author(s):  
Francesca Kim ◽  
Tong Chen ◽  
Trevor Burgess ◽  
Prakash Rasie ◽  
Tim Luca Selinger ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Dna Nanostructures ◽  
Polyaspartic Acid ◽  
Liquid Precursor

Nanopatterned DNA–polyaspartic acid (pAsp) conjugates guide the growth of calcium phosphate minerals in the polymer-induced liquid precursor (PILP) method.

Impact of Biphasic Calcium Phosphate Bioceramics on Osteoporotic Hip Bone Mineralization In Vivo Six Months after Implantation

2016 ◽  
Vol 721 ◽  
pp. 229-233 ◽  
Author(s):  
Sandris Petronis ◽  
Janis Locs ◽  
Vita Zalite ◽  
Mara Pilmane ◽  
Andrejs Skagers ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Tricalcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Bone Mineralization ◽  
Bone Substitutes ◽  
Tissue Response ◽  
Control Group ◽  
Osteoporotic Bone ◽  
Local Recovery

Calcium bone substitutes are successfully used for local recovery of osteoporotic bone and filling of bone defects. Previous studies revieled that biphasic calcium phosphate (BCP) show better bioactivity in compare to pure β-tricalcium phosphate or hydroxyapatite. Also increased porosity of material promotes better bone tissue response. Aim of this experiment was to evaluate immunohistologically response of osteoporotic bone of experimental animal to implantation of granules with hydroxyapatite/β-tricalcium phosphate (HAp/β-TCP) ratio of 90/10. Calcium phosphate (CaP) was synthesized by aqueous precipitation technique from calcium hydroxide and phosphoric acid. Bioceramic granules in size range from 1.0 to 1.4 mm were prepared with nanopore sizes around 200 nm. We used nine female rabbits with induced osteoporosis in this experiment. Six animals in study group underwent implantation of BCP in hip bone defect and three animals in control group left without BCP implantation. After 6 months animals were euthanized, bone samples collected and proceeded for detection of bone activity and repair markers: osteocalcin (OC), osteopontin (OP) and osteoprotegerin (OPG). Controls showed the presence of experimental bone osteoporosis. In experimental group bone showed partially resorbed bioceramic granules and in some samples new bone formation near the granuli was observed. Increase of OC and OPG up to twice as to compare to control group were detected as well. Implantation of BCP granules in osteoporotic rabbit bone increases expression of OC and OPG indicating the activation of osteoblastogenesis and bone mineralization in vivo.

scholarly journals Novel Biodegradable Composite of Calcium Phosphate Cement and the Collagen I Mimetic P-15 for Pedicle Screw Augmentation in Osteoporotic Bone

Biomedicines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1392
Author(s):  
Harald Krenzlin ◽  
Andrea Foelger ◽  
Volker Mailänder ◽  
Christopher Blase ◽  
Marc Brockmann ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Pedicle Screw ◽  
Calcium Phosphate Cement ◽  
Pedicle Screws ◽  
Osteoblastic Differentiation ◽  
Collagen I ◽  
Osteoporotic Bone ◽  
Mineral Density ◽  
Screw Augmentation ◽  
Pullout Loads

Osteoporotic vertebral fractures often necessitate fusion surgery, with high rates of implant failure. We present a novel bioactive composite of calcium phosphate cement (CPC) and the collagen I mimetic P-15 for pedicle screw augmentation in osteoporotic bone. Methods involved expression analysis of osteogenesis-related genes during osteoblastic differentiation by RT-PCR and immunostaining of osteopontin and Ca2+ deposits. Untreated and decalcified sheep vertebrae were utilized for linear pullout testing of pedicle screws. Bone mineral density (BMD) was measured using dual-energy X-ray absorptiometry (DEXA). Expression of ALPI II (p < 0.0001), osteopontin (p < 0.0001), RUNX2 (p < 0.0001), and osteocalcin (p < 0.0001) was upregulated after co-culture of MSC with CPC-P-15. BMD was decreased by 28.75% ± 2.6%. Pullout loads in untreated vertebrae were 1405 ± 6 N (p < 0.001) without augmentation, 2010 ± 168 N (p < 0.0001) after augmentation with CPC-P-15, and 2112 ± 98 N (p < 0.0001) with PMMA. In decalcified vertebrae, pullout loads were 828 ± 66 N (p < 0.0001) without augmentation, 1324 ± 712 N (p = 0.04) with PMMA, and 1252 ± 131 N (p < 0.0078) with CPC-P-15. CPC-P-15 induces osteoblastic differentiation of human MES and improves pullout resistance of pedicle screws in osteoporotic and non-osteoporotic bone.

scholarly journals Calcium phosphate crystal forms in human jaw bones of changed osteoporotic structure

2011 ◽  
Vol 58 (1) ◽  
pp. 23-28
Author(s):  
Srdjan Postic
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphates ◽  
Chemical Compounds ◽  
Osteoporotic Bone ◽  
X Ray Diffraction ◽  
Crystal Forms ◽  
Jaw Bone ◽  
Jaw Bones ◽  
Human Cadavers ◽  
Hydrated Calcium

Introduction. Calcium phosphates are chemical compounds that can be found in various forms within nature, as well as in human jaws and bones. The aim of this study was to assess the structure of solid chemical compounds which form the structure of normal and osteoporotic jaw-bones. Materials and Methods. The jaw-bones taken from human cadavers were used in the study. Crystalographic forms of calcium phosphate, in the samples of human jaw-bone, were determined using X-ray diffraction technique. The experimental bone samples originated from osteoporotic jaw-bone of cadavers while control samples were taken from dentate jaw-bones of non-osteoporotic cadavers. Results. The results of this study showed that hydroxyapatite was the only phase determined in control non-osteoporotic bone samples. In experimental (osteoporotic) bone samples, the same phase was registered, as well as calcium monophosphate and hydrated calcium phosphate, registered as increments of values on ? axis. Conclusion. Hydroxyapatite was the only compound detected in normal bone while osteoporotic bone contained others crystallographic forms of calcium phosphates.

Strontium-modified premixed calcium phosphate cements for the therapy of osteoporotic bone defects

2018 ◽  
Vol 65 ◽  
pp. 475-485 ◽  
Author(s):  
A. Lode ◽  
C. Heiss ◽  
G. Knapp ◽  
J. Thomas ◽  
B. Nies ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Defects ◽  
Osteoporotic Bone ◽  
Calcium Phosphate Cements
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