scholarly journals Netrin-1 Promotes Inflammation Resolution to Achieve Endothelialization of Small-Diameter Tissue Engineering Blood Vessels by Improving Endothelial Progenitor Cells Function In Situ

2017 ◽  
Vol 4 (12) ◽  
pp. 1700278 ◽  
Author(s):  
Yanzhao Li ◽  
Simin Wan ◽  
Ge Liu ◽  
Wang Cai ◽  
Da Huo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Small Diameter ◽  
Endothelial Progenitor ◽  
Inflammation Resolution

scholarly journals In vitro cytocompatibility evaluation of collagen based scaffolds using human endothelial progenitor cells for vascular tissue engineering

2014 ◽  
Vol 1 (1-4) ◽  
pp. 10-16 ◽  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Human Umbilical Cord ◽  
Collagen Scaffolds ◽  
Endothelial Progenitor

Vascular tissue engineering attempts to grow blood vessels through the use of different scaffolds that allows vascular cells such as endothelial cells to form networks and organized in vascular tissue. Various biomaterials are used to produce scaffolds that allow growth and differentiation of stem cells; depending on the cell type and applications some materials are more suitable than other. The aim of this study was to evaluate the cytocompatibility of collagen based scaffolds and to assess the capacity of endothelial progenitor cells (EPC) isolated from human umbilical cord to form vascular networks on these scaffolds. Our results show that after 5 days in culture with collagen scaffolds, the EPC remained viable, a sign of biocompatibility with the 3D scaffolds. Scanning electron microscopy showed that in the collagen scaffolds EPC organize within networks and presents an abundant extracellular matrix that strengthen the links between them. When EPC were cultured on collagenchitosan scaffolds, they are more adherent to the scaffolds compared with collagen, exibiting a good capacity to form networks. This study shows that the collagen and collagen-chitosan scaffolds are not cytotoxic for EPC and they provide the possibility of being used in vascular tissue engineering to help creating blood vessels.

In situ capture technology of endothelial progenitor cells

2013 ◽  
Vol 22 (3) ◽  
pp. e47
Author(s):  
Takehisa Matsuda ◽  
Shu Takabatake ◽  
Masakazu Yamagishi
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor

miR-126 modulates angiogenic growth parameters of peripheral blood endothelial progenitor cells

2015 ◽  
Vol 396 (3) ◽  
pp. 245-252 ◽  
Author(s):  
Sebastian M. Goerke ◽  
Lena S. Kiefer ◽  
G. Björn Stark ◽  
Filip Simunovic ◽  
Günter Finkenzeller
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Growth Parameters ◽  
Therapeutic Option ◽  
Tube Formation ◽  
Loss Of Function ◽  
Endothelial Progenitor

Abstract Vascularization plays an important role in tissue engineering applications. It is known that implantation of differentiated endothelial cells or endothelial progenitor cells (EPCs) from cord blood (cbEPCs) gives rise to the formation of a complex functional neovasculature, whereas EPCs isolated from peripheral blood (pbEPCs) have a limited capability to form blood vessels upon implantation. MicroRNA-126 (miR-126) has been shown to have pro-angiogenic effects in vivo. In this study, we investigated whether modulation of miR-126 expression in pbEPCs may alter their angiogenic properties. Gain of function and loss of function experiments revealed that miR-126 has anti-angiogenic effects in pbEPCs. Overexpression of miR-126 resulted in decreased proliferation, migration, invasion and tube formation, while inhibition of miR-126 induced the opposite effects. However, modulation of miR-126 expression did not influence apoptotic susceptibility of pbEPCs. This study provides evidence that inhibition of miR-126 improves angiogenesis-related growth parameters in pbEPCs and may represent a therapeutic option to ameliorate the angiogenic and vasculogenic properties of pbEPCs.

scholarly journals Effects of adipose-derived stromal cells and endothelial progenitor cells on adipose transplant survival and angiogenesis

2020 ◽  
Author(s):  
Xian Zhao ◽  
Fengshan Gan ◽  
Liu Liu ◽  
Qingzhu Zhou ◽  
Wenli Huang ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Migration And Invasion ◽  
Vascular Endothelial ◽  
Paracrine Signaling ◽  
Endothelial Progenitor ◽  
Endothelial Markers

Abstract Background: A paracrine mechanism is thought to mediate the proangiogenic capacity of adipose-derived stromal/stem cells (ASCs). However, the precise mechanism by which ASCs promote the formation of blood vessels by endothelial progenitor cells (EPCs) is unclear.Methods: We cocultured ASCs with EPCs at various concentrations to study the effects on angiogenesis. The supernatant from cultured ASCs was cocultured with EPCs to evaluate the effects on the expression of vascular endothelial markers in EPCs, as well as capacity for migration and invasion. We then mixed ASCs with EPCs and transplanted them with adipose tissue into New Zealand white rabbits to evaluate the effects on angiogenesis in adipose tissue grafts.Results: As the relative abundance of ASCs cocultured with EPCs increased, the rate of angiogenesis among EPCs decreased. The supernatant from ASC cultures increased the migration and invasion of EPCs and upregulated the expression of vascular endothelial markers in EPCs. In vivo, ASCs promoted the production of blood vessels by EPCs.Conclusions: ASCs are not recruited as structural components of blood vessels but do appear to regulate endothelial progenitor-mediated angiogenesis. The results obtained show that ASC paracrine signaling promotes the formation of blood vessels by EPCs. ASC paracrine signaling appears to promote angiogenesis by increasing the migration and invasion of EPCs and simultaneously upregulating the expression of vascular endothelial markers in EPCs. The results of in vivo experiments showed that ASCs promote the formation of blood vessels in EPC cultures.

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