scholarly journals Endothelial progenitor cells contribute to the development of ovarian carcinoma tumor blood vessels

2014 ◽  
Vol 7 (5) ◽  
pp. 1511-1514 ◽  
Author(s):  
DORIN GRIGORAS ◽  
LAURENŢIU PIRTEA ◽  
RALUCA AMALIA CEAUSU
Keyword(s):  
Ovarian Carcinoma ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor ◽  
Tumor Blood Vessels ◽  
Carcinoma Tumor

scholarly journals Effects of adipose-derived stromal cells and endothelial progenitor cells on adipose transplant survival and angiogenesis

2020 ◽  
Author(s):  
Xian Zhao ◽  
Fengshan Gan ◽  
Liu Liu ◽  
Qingzhu Zhou ◽  
Wenli Huang ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Migration And Invasion ◽  
Vascular Endothelial ◽  
Paracrine Signaling ◽  
Endothelial Progenitor ◽  
Endothelial Markers

Abstract Background: A paracrine mechanism is thought to mediate the proangiogenic capacity of adipose-derived stromal/stem cells (ASCs). However, the precise mechanism by which ASCs promote the formation of blood vessels by endothelial progenitor cells (EPCs) is unclear.Methods: We cocultured ASCs with EPCs at various concentrations to study the effects on angiogenesis. The supernatant from cultured ASCs was cocultured with EPCs to evaluate the effects on the expression of vascular endothelial markers in EPCs, as well as capacity for migration and invasion. We then mixed ASCs with EPCs and transplanted them with adipose tissue into New Zealand white rabbits to evaluate the effects on angiogenesis in adipose tissue grafts.Results: As the relative abundance of ASCs cocultured with EPCs increased, the rate of angiogenesis among EPCs decreased. The supernatant from ASC cultures increased the migration and invasion of EPCs and upregulated the expression of vascular endothelial markers in EPCs. In vivo, ASCs promoted the production of blood vessels by EPCs.Conclusions: ASCs are not recruited as structural components of blood vessels but do appear to regulate endothelial progenitor-mediated angiogenesis. The results obtained show that ASC paracrine signaling promotes the formation of blood vessels by EPCs. ASC paracrine signaling appears to promote angiogenesis by increasing the migration and invasion of EPCs and simultaneously upregulating the expression of vascular endothelial markers in EPCs. The results of in vivo experiments showed that ASCs promote the formation of blood vessels in EPC cultures.

Effects of Angiopoietin-1 on Inflammatory Injury in Endothelial Progenitor Cells and Blood Vessels

2014 ◽  
Vol 14 (2) ◽  
pp. 128-135 ◽  
Author(s):  
Yi-Qing Wang ◽  
Jing-Jin Song ◽  
Xiao Han ◽  
Yi-Ye Liu ◽  
Xi-Huang Wang ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor ◽  
Inflammatory Injury ◽  
Angiopoietin 1

scholarly journals Endothelial Progenitor Cells in Tumor Angiogenesis: Another Brick in the Wall

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Marina Marçola ◽  
Camila Eleuterio Rodrigues
Keyword(s):  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Tumor Angiogenesis ◽  
Main Function ◽  
Endothelial Progenitor ◽  
Vascular Regeneration ◽  
Undifferentiated Cells ◽  
Vasculogenesis And Angiogenesis ◽  
Tumor Growth And Metastasis

Until 15 years ago, vasculogenesis, the formation of new blood vessels from undifferentiated cells, was thought to occur only during embryonic development. The discovery of circulating cells that are able to promote vascular regeneration and repair—the so-called endothelial progenitor cells (EPCs)—changed that, and EPCs have since been studied extensively. It is already known that EPCs include many subtypes of cells that play a variety of roles in promoting vascular growth. Some EPCs are destined to differentiate into endothelial cells, whereas others are capable of promoting and sustaining angiogenesis through paracrine mechanisms. Vasculogenesis and angiogenesis might constitute complementary mechanisms for postnatal neovascularization, and EPCs could be at the core of this process. Although the formation of new blood vessels from preexisting vasculature plays a beneficial role in many physiological processes, such as wound healing, it also contributes to tumor growth and metastasis. However, many aspects of the role played by EPCs in tumor angiogenesis remain unclear. This review aims to address the main aspects of EPCs differentiation and certain characteristics of their main function, especially in tumor angiogenesis, as well as the potential clinical applications.

Comparative Analysis of Methods for Assessment of Circulating Endothelial Progenitor Cells Indicated Heterogeneity of Subpopulations at Various Stages of Differentiation.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4134-4134
Author(s):  
Varda R. Deutsch ◽  
Haim Shmilovich ◽  
Anastasia Abashidze ◽  
Hylton Miller ◽  
Gad Keren ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Comparative Analysis ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Mononuclear Cells ◽  
Therapeutic Potential ◽  
Proliferative Capacity ◽  
Endothelial Progenitor ◽  
Humoral Factors

Abstract The cells of the vasculature are derived from pluripotent stem cells, known as hemangioblasts, which also give rise to blood. Angiogenesis, the formation of new blood vessels is considered to result from the proliferation and migration of mature endothelial cells from existing blood vessels or from the recently described, but not yet well characterized endothelial progenitor cells (EPCs). The number and properties of EPC in disease states is of considerable interest due to the promising therapeutic potential of these cells. EPCs have been shown to be mobilized from the bone marrow and contribute to angiogenesis following vascular injury, organ ischaemia and tumor progression. However, mechanisms that drive the EPC response to injury remain elusive and the lack of definitions of EPC subpopulations and the many methods used by different groups to identify these cells make correlation of results difficult. To begin to understand the potential of these cells, we performed a comparative analysis of several methods used for circulating EPC assessment in 40 healthy individuals (mean age of 33±9 years) and correlated them with humoral factors known to influence their numbers. Peripheral blood mononuclear cells were obtained and evaluated by flow cytometeric analysis with antibodies to CD34, CD45, CD133 and KDR, and the remaining cells grown under endothelial cell conditions for assessment of colony forming unit (CFU) numbers and adhesive properties. Levels of circulating vascular endothelial growth factor (VEGF), erythropoietin and C-reactive protein were determined and correlated with each of the EPC markers. CFU numbers did not correlate with the number of CD34/KDR (VEGFR2) or CD34/CD133/KDR positive cells and negatively correlated with CD34/CD133 (which includes hematopoietic progenitors). CD34/KDR number correlated with CD34/CD133/KDR but not with CD34/CD133. Only the VEGFR2 positive cells populations (CD34/KDR and CD34/CD133/KDR) correlated with VEGF serum levels. The number of EPC adhering to fibronectin and endothelial-cells correlated with CFU numbers but not with EPC membrane markers. Our results indicate for the first time that EPC, like hematopoietic precursors may be a heterogeneous cell population comprised of progenitors at various stages of differentiation, having varied proliferative capacity. This could explain the lack of correlation in results obtained using different methods for quantitatively assessing the numbers of circulating EPC. These data also suggest that co-expression of CD34, CD133and KDR-VEGFR2 is appropriate for defining a population of circulating EPC whereas CFU are more likely to reflect the proliferative capacity of the progenitors.

scholarly journals Effects of adipose-derived stromal cells and endothelial progenitor cells on adipose transplant survival and angiogenesis

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0261498
Author(s):  
Fengshan Gan ◽  
Liu Liu ◽  
Qingzhu Zhou ◽  
Wenli Huang ◽  
Xinwei Huang ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Tube Formation ◽  
Migration And Invasion ◽  
Endothelial Progenitor ◽  
Angiogenic Potential ◽  
Adipose Derived Stromal Cells

Background A paracrine mechanism is thought to mediate the proangiogenic capacity of adipose-derived stromal/stem cells (ASCs). However, the precise mechanism by which ASCs promote the formation of blood vessels by endothelial progenitor cells (EPCs) is unclear. Methods The EPCs-ASCs cocultures prepared in different ratios were subjected to tube formations assay to verify whether ASCs could directly participate in the tube genesis. The supernatant from cultured ASCs was used to stimulate EPCs to evaluate the effects on the angiogenic property of EPCs, as well as capacity for migration and invasion. A coculture model with transwell chamber were used to explore the regulation of angiogenesis markers expression in EPCs by ASCs. We then mixed ASCs with EPCs and transplanted them with adipose tissue into nude mice to evaluate the effects on angiogenesis in adipose tissue grafts. Results In the EPCs-ASCs cocultures, the tube formation was significantly decreased as the relative abundance of ASCs increased, while the ASCs was found to migrate and integrated into the agglomerates formed by EPCs. The supernatant from ASCs cultures promoted the migration and invasion of EPCs and the ability to form capillary-like structures. The expression of multiple angiogenesis markers in EPCs were significantly increased when cocultured with ASCs. In vivo, ASCs combined with EPC promoted vascularization in the fat transplant. Immunofluorescence straining of Edu and CD31 indicated that the Edu labeled EPC did not directly participate in the vascularization inside the fat tissue. Conclusions ADSC can participate in the tube formation of EPC although it cannot form canonical capillary structures. Meanwhile, Soluble factors secreted by ASCs promotes the angiogenic potential of EPCs. ASCs paracrine signaling appears to promote angiogenesis by increasing the migration and invasion of EPCs and simultaneously upregulating the expression of angiogenesis markers in EPCs. The results of in vivo experiments showed that ASCs combined with EPCs significantly promote the formation of blood vessels in the fat implant. Remarkably, EPCs may promote angiogenesis by paracrine regulation of endogenous endothelial cells (ECs) rather than direct participation in the formation of blood vessels.

scholarly journals In vitro cytocompatibility evaluation of collagen based scaffolds using human endothelial progenitor cells for vascular tissue engineering

2014 ◽  
Vol 1 (1-4) ◽  
pp. 10-16 ◽  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Human Umbilical Cord ◽  
Collagen Scaffolds ◽  
Endothelial Progenitor

Vascular tissue engineering attempts to grow blood vessels through the use of different scaffolds that allows vascular cells such as endothelial cells to form networks and organized in vascular tissue. Various biomaterials are used to produce scaffolds that allow growth and differentiation of stem cells; depending on the cell type and applications some materials are more suitable than other. The aim of this study was to evaluate the cytocompatibility of collagen based scaffolds and to assess the capacity of endothelial progenitor cells (EPC) isolated from human umbilical cord to form vascular networks on these scaffolds. Our results show that after 5 days in culture with collagen scaffolds, the EPC remained viable, a sign of biocompatibility with the 3D scaffolds. Scanning electron microscopy showed that in the collagen scaffolds EPC organize within networks and presents an abundant extracellular matrix that strengthen the links between them. When EPC were cultured on collagenchitosan scaffolds, they are more adherent to the scaffolds compared with collagen, exibiting a good capacity to form networks. This study shows that the collagen and collagen-chitosan scaffolds are not cytotoxic for EPC and they provide the possibility of being used in vascular tissue engineering to help creating blood vessels.

scholarly journals Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels

Blood ◽  
2008 ◽  
Vol 111 (3) ◽  
pp. 1302-1305 ◽  
Author(s):  
Patrick Au ◽  
Laurence M. Daheron ◽  
Dan G. Duda ◽  
Kenneth S. Cohen ◽  
James A. Tyrrell ◽  
...  
Keyword(s):  
Cord Blood ◽  
Blood Vessels ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Peripheral Blood ◽  
Endothelial Progenitor ◽  
Adult Peripheral Blood

Abstract Tissue engineering requires formation of a de novo stable vascular network. Because of their ability to proliferate, differentiate into endothelial cells, and form new vessels, blood-derived endothelial progenitor cells (EPCs) are attractive source of cells for use in engineering blood vessels. However, the durability and function of EPC-derived vessels implanted in vivo are unclear. To this end, we directly compared formation and functions of tissue-engineered blood vessels generated by peripheral blood– and umbilical cord blood–derived EPCs in a model of in vivo vasculogenesis. We found that adult peripheral blood EPCs form blood vessels that are unstable and regress within 3 weeks. In contrast, umbilical cord blood EPCs form normal-functioning blood vessels that last for more than 4 months. These vessels exhibit normal blood flow, perm-selectivity to macromolecules, and induction of leukocyte-endothelial interactions in response to cytokine activation similar to normal vessels. Thus, umbilical cord blood EPCs hold great therapeutic potential, and their use should be pursued for vascular engineering.

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