scholarly journals Continuous Flow Bioamination of Ketones in Organic Solvents at Controlled Water Activity using Immobilized ω‐Transaminases

2020 ◽  
Vol 362 (9) ◽  
pp. 1858-1867 ◽  
Author(s):  
Wesley Böhmer ◽  
Alexey Volkov ◽  
Karim Engelmark Cassimjee ◽  
Francesco G. Mutti
Keyword(s):  
Organic Solvents ◽  
Water Activity ◽  
Continuous Flow

scholarly journals Influence of Water Activity on Protease Adsorbed on Biochar in Organic Solvents

2017 ◽  
Vol 6 (4) ◽  
pp. 96 ◽  
Author(s):  
Hidetaka Noritomi ◽  
Jumpei Nishigami ◽  
Nobuyuki Endo ◽  
Satoru Kato ◽  
Katsumi Uchiyama
Keyword(s):  
Thermal Stability ◽  
Catalytic Activity ◽  
Organic Solvents ◽  
Water Activity ◽  
Initial Rate ◽  
Butyl Ester ◽  
Nitrogen Atmosphere ◽  
Bamboo Charcoal ◽  
Influence Of Water ◽  
Hydrolysis Of

We have found that the organic solvent-resistance of Alpha-chymotrypsin (Alpha-CT) is enhanced by adsorbing Alpha-CT onto bamboo charcoal powder (BCP), which is obtained by pyrolyzing bamboo waste under nitrogen atmosphere, and is markedly dependent on the thermodynamic water activity (aw) in organic solvents. When BCP-adsorbed　Alpha-CT was immersed in acetonitrile at an appropriate water activity, it effectively enhanced the transesterification of N-acetyl-L-tyrosine ethyl ester (N-Ac-Tyr-OEt) with n-butanol (BuOH) to produce N-acetyl-L-tyrosine butyl ester (N-Ac-Tyr-OBu), compared to the hydrolysis of N-Ac-Tyr-OEt with water to give N-acetyl-L-tyrosine (N-Ac-Tyr-OH). When the water activity was 0.28, the initial rate of transesterification catalyzed by BCP-adsorbed Alpha-CT was about sixty times greater than that catalyzed by free Alpha-CT. Regarding the reaction selectivity which is defined as a ratio of the initial rate of transesterification to that of hydrolysis, BCP-adsorbed α-CT was much superior to free Alpha-CT. The catalytic activity of BCP-adsorbed Alpha-CT was markedly dependent on the reaction temperature. Furthermore, concerning the thermal stability at 50 oC, the half-life of BCP-adsorbed Alpha-CT exhibited 3.8-fold, compared to that of free Alpha-CT.

Continuous Flow Synthesis of Chiral Amines in Organic Solvents: Immobilization of E. coli Cells Containing Both ω-Transaminase and PLP

2014 ◽  
Vol 16 (23) ◽  
pp. 6092-6095 ◽  
Author(s):  
Leandro H. Andrade ◽  
Wolfgang Kroutil ◽  
Timothy F. Jamison
Keyword(s):  
Organic Solvents ◽  
Continuous Flow ◽  
Chiral Amines ◽  
Flow Synthesis ◽  
E Coli

scholarly journals Probing batch and continuous flow reactions in organic solvents: Granulicella tundricola hydroxynitrile lyase (GtHNL)

2020 ◽  
Vol 10 (11) ◽  
pp. 3613-3621 ◽  
Author(s):  
José Coloma ◽  
Yann Guiavarc'h ◽  
Peter-Leon Hagedoorn ◽  
Ulf Hanefeld
Keyword(s):  
Organic Solvents ◽  
Continuous Flow ◽  
Enantioselective Synthesis ◽  
Hydroxynitrile Lyase

Granulicella tundricola hydroxynitrile lyase (GtHNL) is a manganese dependent cupin which catalyses the enantioselective synthesis of (R)-cyanohydrins.

scholarly journals Solvent Resistant O2 Sensor Integrated in Pressured Flow Reactors

Proceedings ◽  
2019 ◽  
Vol 2 (13) ◽  
pp. 994
Author(s):  
Philipp Sulzer ◽  
René Lebl ◽  
Torsten Mayr
Keyword(s):  
Organic Solvents ◽  
Continuous Flow ◽  
Oxygen Sensor ◽  
Dynamic Range ◽  
Flow Reactor ◽  
State Of The Art ◽  
Flow Reactors ◽  
Optical Oxygen Sensor ◽  
Continuous Flow Reactors

In this contribution we present an optical oxygen sensor that can easily be integrated into continuous flow reactors. The sensors are pressure resistant to up to 8 bars and are able to continuously measure oxygen for several hours in organic solvents. The dynamic range was evaluated up to 42 mmol/L O2 and several calibrations showed the sensors to perform linearly according to Stern-Volmer, which allows convenient two-point calibrations. By using state-of-the-art HPLC connectors and materials the sensor can be implemented at virtually every point of a standard flow reactor. Readout is conducted via a 4-channel USB phase fluorimeter.

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