scholarly journals A Biocatalytic One-Pot Approach for the Preparation of Lignin Oligomers Using an Oxidase/Peroxidase Cascade Enzyme System

2017 ◽  
Vol 359 (19) ◽  
pp. 3354-3361 ◽  
Author(s):  
Mohamed H. M. Habib ◽  
Peter J. Deuss ◽  
Nikola Lončar ◽  
Milos Trajkovic ◽  
Marco W. Fraaije
Keyword(s):  
Enzyme System ◽  
One Pot

A Procedure to Design One-Pot Multi-enzyme System for Industrial CDP-Choline Production

2021 ◽  
Author(s):  
Cheng Zheng ◽  
Rongxin Miao ◽  
Yingmiao Liu ◽  
Yang Cao ◽  
Dong Liu ◽  
...  
Keyword(s):  
Enzyme System ◽  
One Pot

scholarly journals Efficient Production of 3′-Sialyllactose by Single Whole-Cell in One-Pot Biosynthesis

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 932
Author(s):  
Zhongkui Li ◽  
Xiangsong Chen ◽  
Zhijian Ni ◽  
Lixia Yuan ◽  
Lijie Sun ◽  
...  
Keyword(s):  
Surfactant Concentration ◽  
Enzyme System ◽  
Efficient Production ◽  
Optimal Conditions ◽  
One Pot ◽  
Whole Cell ◽  
Economic Competitiveness ◽  
Enzyme Cascade ◽  
L System ◽  
Conversion Temperature

Sialyllactose (SL) is one of the most important acidic oligosaccharides in human milk, which plays an important role in the health of infants. In this work, an efficient multi-enzyme cascade was developed in a single whole cell to produce 3′-SL. We constructed two compatible plasmids with double cloning sites to co-express four genes. Different combinations were assessed to verify the optimal catalytic ability. Then, the conversion temperature, pH, and stability under the optimal temperature and pH were investigated. Moreover, the optimal conversion conditions and surfactant concentration were determined. By using the optimal conditions (35 °C, pH 7.0, 20 mM polyphosphate, 10 mM cytidine monophosphate (CMP), 20 mM MgCl2), 25 mL and 4 L conversion systems were carried out to produce 3′-SL. Similar results were obtained between different volume conversion reactions, which led the maximum production of 3′-SL to reach 53 mM from 54.2 mM of sialic acid (SA) in the 25 mL system and 52.8 mM of 3′-SL from 53.8 mM of SA in the 4 L system. These encouraging results demonstrate that the developed single whole-cell multi-enzyme system exhibits great potential and economic competitiveness for the manufacture of 3′-SL.

A one-pot two-enzyme system on the production of high value-added D-allulose from Jerusalem artichoke tubers

2020 ◽  
Vol 88 ◽  
pp. 90-96 ◽  
Author(s):  
Ping Zhu ◽  
Yan Zeng ◽  
Peng Chen ◽  
Yan Men ◽  
Jiangang Yang ◽  
...  
Keyword(s):  
Enzyme System ◽  
Jerusalem Artichoke ◽  
Value Added ◽  
One Pot

A new multi-enzyme system for a one-pot synthesis of sialyl oligosaccharides: Combined use of β-galactosidase and α(26)-sialyltransferase coupled with regeneration in situ of CMP-sialic acid

1993 ◽  
Vol 34 (19) ◽  
pp. 3091-3094 ◽  
Author(s):  
Guido F. Herrmann ◽  
Yoshitaka Ichikawa ◽  
Christian Wandrey ◽  
Federico C.A. Gaeta ◽  
James C. Paulson ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Enzyme System ◽  
One Pot ◽  
One Pot Synthesis ◽  
Combined Use

One-pot process of 2′-deoxyguanylic acid catalyzed by a multi-enzyme system

2015 ◽  
Vol 20 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Yanyu Li ◽  
Qingbao Ding ◽  
Ling Ou ◽  
Yahui Qian ◽  
Jiao Zhang
Keyword(s):  
Enzyme System ◽  
One Pot ◽  
Acid Catalyzed

Biocatalytic asymmetric synthesis of l-phosphinothricin using a one-pot three enzyme system and a continuous substrate fed-batch strategy

2020 ◽  
Vol 589 ◽  
pp. 117239 ◽  
Author(s):  
Haisheng Zhou ◽  
Lijun Meng ◽  
Xinjian Yin ◽  
Yayun Liu ◽  
Jianping Wu ◽  
...  
Keyword(s):  
Asymmetric Synthesis ◽  
Enzyme System ◽  
Fed Batch ◽  
One Pot
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