scholarly journals Point‐of‐Care Platform for Rapid Multiplexed Detection of SARS‐CoV‐2 Variants and Respiratory Pathogens

2022 ◽  
pp. 2101013
Author(s):  
Alexander Y. Trick ◽  
Fan‐En Chen ◽  
Liben Chen ◽  
Pei‐Wei Lee ◽  
Alexander C. Hasnain ◽  
...  
Keyword(s):  
Point Of Care ◽  
Respiratory Pathogens ◽  
Multiplexed Detection

scholarly journals Multiplexed Reverse Transcriptase PCR Assay for Identification of Viral Respiratory Pathogens at the Point of Care

2007 ◽  
Vol 45 (11) ◽  
pp. 3498-3505 ◽  
Author(s):  
S. E. Letant ◽  
J. I. Ortiz ◽  
L. F. Bentley Tammero ◽  
J. M. Birch ◽  
R. W. Derlet ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Point Of Care ◽  
Respiratory Pathogens ◽  
Pcr Assay ◽  
Reverse Transcriptase Pcr ◽  
Viral Respiratory

scholarly journals Risk of bias and limits of reporting in diagnostic accuracy studies for commercial point-of-care tests for respiratory pathogens

2018 ◽  
Vol 146 (6) ◽  
pp. 747-756
Author(s):  
J.M. Hughes ◽  
C. Penney ◽  
S. Boyd ◽  
P. Daley
Keyword(s):  
Diagnostic Accuracy ◽  
Test Performance ◽  
Point Of Care ◽  
Risk Of Bias ◽  
Financial Impact ◽  
Test Accuracy ◽  
Respiratory Pathogens ◽  
Study Results ◽  
Group A ◽  
Point Of Care Tests

AbstractCommercial point-of-care (POC) diagnostic tests for Group A Streptococcus, Streptococcus pneumoniae, and influenza virus have large potential diagnostic and financial impact. Many published reports on test performance, often funded by diagnostics companies, are prone to bias. The Standards for Reporting of Diagnostic Accuracy (STARD 2015) are a protocol to encourage accurate, transparent reporting. The Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool evaluates risk of bias and transportability of results. We used these tools to evaluate diagnostic test accuracy studies of POC studies for three respiratory pathogens. For the 96 studies analysed, compliance was <25% for 14/34 STARD 2015 standards, and 3/7 QUADAS-2 domains showed a high risk of bias. All reports lacked reporting of at least one criterion. These biases should be considered in the interpretation of study results.

Towards a point-of-care nanoplasmonic biosensor for rapid and multiplexed detection of pathogenic infections

2018 ◽  
Author(s):  
Maria Soler Aznar ◽  
Xiaokang Li ◽  
Alexander Belushkin ◽  
Hatice Altug ◽  
Filiz Yesilköy
Keyword(s):  
Point Of Care ◽  
Multiplexed Detection

scholarly journals Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 97 ◽  
Author(s):  
Peijun He ◽  
Ioannis Katis ◽  
Robert Eason ◽  
Collin Sones
Keyword(s):  
Bacterial Infections ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Direct Write ◽  
Multiplexed Detection ◽  
Amyloid A ◽  
Flow Devices ◽  
Laser Direct Write

Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure.

A hydrogel sensor-based microfluidic platform for the quantitative and multiplexed detection of fertility markers for point-of-care immunoassays

2019 ◽  
Vol 11 (12) ◽  
pp. 1639-1650 ◽  
Author(s):  
Satish Kalme ◽  
Srinivasan Kandaswamy ◽  
Anusha Chandrasekharmath ◽  
Reeta Katiyar ◽  
Gokul Prasath Rajamanickam ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Point Of Care ◽  
Microfluidic Platform ◽  
Multiplexed Detection ◽  
Hydrogel Particle ◽  
Multiplexed Immunoassay ◽  
Porous Hydrogel

We report a new point-of-care, multiplexed immunoassay platform based on 3D porous hydrogel particle sensors embedded into a plastic microfluidic device.

scholarly journals Multiplexed detection of respiratory pathogens with a portable analyzer in a “raw-sample-in and answer-out” manner

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Nan Li ◽  
Minjie Shen ◽  
Jiajia Liu ◽  
Li Zhang ◽  
Huili Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Bacterial Species ◽  
Magnetic Bead ◽  
Acid Extraction ◽  
Respiratory Pathogens ◽  
Nucleic Acid Extraction ◽  
Multiplexed Detection ◽  
Portable Analyzer

AbstractCoronavirus disease 2019 (COVID-19) has emerged, rapidly spread and caused significant morbidity and mortality worldwide. There is an urgent public health need for rapid, sensitive, specific, and on-site diagnostic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In this study, a fully integrated and portable analyzer was developed to detect SARS-CoV-2 from swab samples based on solid-phase nucleic acid extraction and reverse transcription loop-mediated isothermal amplification (RT-LAMP). The swab can be directly inserted into a cassette for multiplexed detection of respiratory pathogens without pre-preparation. The overall detection process, including swab rinsing, magnetic bead-based nucleic acid extraction, and 8-plex real-time RT-LAMP, can be automatically performed in the cassette within 80 min. The functionality of the cassette was validated by detecting the presence of a SARS-CoV-2 pseudovirus and three other respiratory pathogens, i.e., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. The limit of detection (LoD) for the SARS-CoV-2 pseudovirus was 2.5 copies/μL with both primer sets (N gene and ORF1ab gene), and the three bacterial species were successfully detected with an LoD of 2.5 colony-forming units (CFU)/μL in 800 μL of swab rinse. Thus, the analyzer developed in this study has the potential to rapidly detect SARS-CoV-2 and other respiratory pathogens on site in a “raw-sample-in and answer-out” manner.

scholarly journals An algorithmic approach to the use of rapid molecular diagnostics for SARS-CoV-2, influenza viruses, and other respiratory pathogens during an unprecedented respiratory season

2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S130-S130
Author(s):  
A S Maris ◽  
L Tao ◽  
C W Stratton ◽  
R M Humphries ◽  
J E Schmitz
Keyword(s):  
Point Of Care ◽  
Random Access ◽  
Access Point ◽  
Clinical Situation ◽  
Influenza Viruses ◽  
Respiratory Pathogen ◽  
Respiratory Pathogens ◽  
Test Volume ◽  
Dumpster Diving ◽  
Pathogen Testing

Abstract Introduction/Objective The COVID-19 pandemic exacerbated deficiencies of testing personnel, reagents, supplies and disposables, instruments, and automation in many clinical laboratories. Upon entering respiratory season, a strategy was warranted to optimize laboratory resources when supplies were already limited and expected respiratory season test volume was unknown. An algorithm was devised to prioritize test ordering and TAT based on patient clinical scenario. Methods/Case Report The institutional respiratory season SARS-CoV-2 algorithm was constructed by a multidisciplinary team including infectious disease, infection prevention, laboratory, and IT/LIS leadership. CDC guidance on influenza testing was incorporated. Antigen-based testing was discontinued; only molecular amplification- based platforms with FDA EUA were utilized. Platforms had a range of TAT (20 minutes to 8 hours) and included fully- automated high throughput, rapid random access, point-of-care, and CDC SARS-CoV-2 assays. Test bundles included SARS-CoV-2 (monoplex), or SARS-CoV-2 + fluA&B (triplex), or SARS-CoV-2 + respiratory pathogen panel (multiplex RPP; includes 22 targets, including flu A&B). Results (if a Case Study enter NA) Key factors in the algorithm included whether the patient was outpatient or inpatient, hospital employee or not, symptomatic or not, immunocompetent or immunocompromised, and whether a concurrent order for other respiratory pathogens was included or not. Clinician responses for these factors determined the type of swab collected (wet swab in VTM or dry swab) and how quickly the TAT was indicated for a given patient using a colored-dot sticker system. Priority TAT in decreasing order was symptomatic inpatients, asymptomatic pre- procedure patients, asymptomatic admissions, symptomatic employees, and symptomatic outpatients. Conclusion An algorithm for respiratory pathogen testing during an unprecedented respiratory season prioritizes result TAT to an individual patient’s clinical situation while maximizing laboratory stewardship by eliminating redundant influenza testing and requiring ‘all upfront’ orders to avoid add-on orders that require ‘dumpster diving’ for samples. Limitations include inherent differences in sensitivity, LOD, and specificity when multiple different platforms are utilized to detect the same analytes.

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