scholarly journals Multi‐Scale In Situ Tribological Studies of Surfaces with 3D Textures Fabricated via Two‐Photon Lithography and Replica Molding

2020 ◽  
Vol 7 (13) ◽  
pp. 2000299
Author(s):  
Mahyar Afshar‐Mohajer ◽  
Min Zou
Keyword(s):  
Replica Molding ◽  
Multi Scale ◽  
Two Photon ◽  
3D Textures

scholarly journals Polarity-active NIR probes with strong two-photon absorption and ultrahigh binding affinity of insulin amyloid fibrils

2021 ◽  
Author(s):  
Li Li ◽  
Zheng Lv ◽  
Zhongwei Man ◽  
Zhenzhen Xu ◽  
YuLing Wei ◽  
...  
Keyword(s):  
Neurodegenerative Diseases ◽  
Binding Affinity ◽  
Fluorescent Probes ◽  
Amyloid Fibrils ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Medical Diagnostic

Amyloid fibrils are associated with many neurodegenerative diseases. In-situ and in-vivo visualization of amyloid fibrils is important for medical diagnostic and requires fluorescent probes with both excitation and emission wavelengths in...

Determination of absolute two-photon excitation cross sections by in situ second-order autocorrelation

1995 ◽  
Vol 20 (23) ◽  
pp. 2372 ◽  
Author(s):  
Chris Xu ◽  
Winfried Denk ◽  
Jeffrey Guild ◽  
Watt W. Webb
Keyword(s):  
Cross Sections ◽  
Second Order ◽  
Two Photon ◽  
Photon Excitation ◽  
Excitation Cross Sections ◽  
Two Photon Excitation

scholarly journals The setup and application of the multi-scale in-situ test system for fatigue damage analysis

2018 ◽  
Vol 165 ◽  
pp. 04005
Author(s):  
Yi Shi ◽  
Xiaoguang Yang ◽  
Guolei Miao ◽  
Duoqi Shi
Keyword(s):  
Growth Rate ◽  
Fatigue Crack ◽  
Crack Growth Rate ◽  
Crack Initiation ◽  
Crack Growth ◽  
Test System ◽  
In Situ Test ◽  
Multi Scale ◽  
Macro Scale

This essays aims at introducing the setup for the multi-scale in-situ test system which is applied for the fatigue crack initiation test. The setup of the experiment system is first introduced, including the image capturing system, optical path system, image acquisition and storage system and the three-axis mobile platform. Then the preparation of micro speckle and the corresponding technique for spatial adjustment are improved to realize the DIC measurement in micro scale. Finally three experiments from macro-meso scale to macro scale: fatigue initiation test, the observation of micro crack and the fatigue crack growth rate in macro scale were conducted to verify the application of the system. The test result can indicate the location of crack initiation, the crack growth rate and the evolution of displacement/strain field, etc.

Applying multi-scale silica-like three-dimensional networks in PEO matrix via in-situ crosslinking for high-performance solid composite electrolyte

2021 ◽  
Author(s):  
Jiawei Wu ◽  
Jing Chen ◽  
Xiaodong Wang ◽  
An'an Zhou ◽  
Zhenglong Yang
Keyword(s):  
Solid State ◽  
High Performance ◽  
Low Cost ◽  
Three Dimensional ◽  
Electrochemical Stability ◽  
Composite Electrolyte ◽  
Solid State Electrolyte ◽  
Multi Scale ◽  
In Situ Crosslinking

For the higher safety and energy density, solid-state electrolyte with better mechanical strength, thermal and electrochemical stability is a perfect choice. To improve the performance of PEO, usage of low-cost...

A New Pre-Embedding Immunogold Method That Permits to Obtain a Very High Signal with a Very Good Ultrastucture

2001 ◽  
Vol 7 (S2) ◽  
pp. 1044-1045
Author(s):  
G. Grondin ◽  
A.R. Bcaudoin
Keyword(s):  
New Technologies ◽  
Immunofluorescence Microscopy ◽  
High Signal ◽  
Sodium Metaperiodate ◽  
Two Photon ◽  
Cacodylate Buffer ◽  
Ultrastructure Preservation ◽  
High Level ◽  
Very High

The ultimate goal of the immunocytochemistry is to get the best signal with the lowest background while preserving ultrastructure. Unfortunaly too often the experimentator faces a compromise between density of the signal and ultrastructure preservation (1). with the advent of hight resolution confocal light microscopy, two photon imaging and other new technologies there is a need to provide the best immunocytochemical complementary results with the high resolution of the electron microscope. But often for exemple the high level of labelling observed by immunofluorescence microscopy is not matched by immunocytochemistry (2). One approach to obtain comparable levels of labelling is to use comparable protocols. We propose here such a new immunogold method. to illustrate our point we used a very convenient biological material, the adherent cells grown on 35 mm plastic culture dishes. The following procedure was applied : Confluent endothelial cells were fixed in situ for 3 hours with the following freshly prepared and filtered solution : 1 % 1-lysine, 4% paraformaldehyde, 0.04% glutaraldehyde, 0.25% sodium metaperiodate in 0.04M sodium cacodylate buffer, pH 7.4.

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