scholarly journals Supercharged Proteins and Polypeptides

2020 ◽  
Vol 32 (20) ◽  
pp. 1905309 ◽  
Author(s):  
Chao Ma ◽  
Anke Malessa ◽  
Arnold J. Boersma ◽  
Kai Liu ◽  
Andreas Herrmann
Keyword(s):  
Supercharged Proteins

scholarly journals SAXS/SANS on Supercharged Proteins Reveals Residue-Specific Modifications of the Hydration Shell

2016 ◽  
Vol 110 (10) ◽  
pp. 2185-2194 ◽  
Author(s):  
Henry S. Kim ◽  
Anne Martel ◽  
Eric Girard ◽  
Martine Moulin ◽  
Michael Härtlein ◽  
...  
Keyword(s):  
Hydration Shell ◽  
Supercharged Proteins

scholarly journals Cellular Uptake Mechanisms and Endosomal Trafficking of Supercharged Proteins

2012 ◽  
Vol 19 (7) ◽  
pp. 831-843 ◽  
Author(s):  
David B. Thompson ◽  
Roberto Villaseñor ◽  
Brent M. Dorr ◽  
Marino Zerial ◽  
David R. Liu
Keyword(s):  
Cellular Uptake ◽  
Endosomal Trafficking ◽  
Supercharged Proteins ◽  
Uptake Mechanisms

scholarly journals Design of Supercharged Proteins to Impart Allosteric Behavior and their Use in Biosensing

2017 ◽  
Vol 112 (3) ◽  
pp. 510a
Author(s):  
Peter Schnatz ◽  
Joseph Brisendine ◽  
Derek Kosciolek ◽  
David Crouse ◽  
Ronald Koder
Keyword(s):  
Supercharged Proteins

scholarly journals Formation of biomolecular condensates in bacteria by tuning protein electrostatics

2020 ◽  
Author(s):  
Vivian Yeong ◽  
Emily G. Werth ◽  
Lewis M. Brown ◽  
Allie C. Obermeyer
Keyword(s):  
Phase Separation ◽  
Protein Electrostatics ◽  
E Coli ◽  
Membrane Barrier ◽  
Condensate Formation ◽  
Protein Components ◽  
Novel Strategy ◽  
Future Utility ◽  
Supercharged Proteins

AbstractBiomolecular condensates provide a strategy for cellular organization without a physical membrane barrier while allowing for dynamic, responsive organization of the cell. To date, very few biomolecular condensates have been identified in prokaryotes, presenting an obstacle to engineering these compartments in bacteria. As a novel strategy for bacterial compartmentalization, protein supercharging and complex coacervation were employed to engineer liquid-like condensates in E. coli. A simple model for the phase separation of supercharged proteins was developed and used to predict intracellular condensate formation. Herein, we demonstrate that GFP-dense condensates formed by expressing GFP variants of sufficient charge in cells are dynamic and enrich specific nucleic acid and protein components. This study provides a fundamental characterization of intracellular phase separation in E. coli driven by protein supercharging and highlights future utility in designing functional synthetic membraneless organelles.

Complex coacervation of supercharged proteins with polyelectrolytes

Soft Matter ◽  
2016 ◽  
Vol 12 (15) ◽  
pp. 3570-3581 ◽  
Author(s):  
Allie C. Obermeyer ◽  
Carolyn E. Mills ◽  
Xue-Hui Dong ◽  
Romeo J. Flores ◽  
Bradley D. Olsen
Keyword(s):  
Complex Coacervation ◽  
Supercharged Proteins
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