Nanopore Arrays for Stable and Functional Free-Standing Lipid Bilayers

2007 ◽  
Vol 19 (24) ◽  
pp. 4466-4470 ◽  
Author(s):  
X. Han ◽  
A. Studer ◽  
H. Sehr ◽  
I. Geissbühler ◽  
M. Di Berardino ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Free Standing ◽  
Nanopore Arrays

Improved stability of free-standing lipid bilayers based on nanoporous alumina films

2010 ◽  
Vol 96 (21) ◽  
pp. 213706 ◽  
Author(s):  
Ayumi Hirano-Iwata ◽  
Tasuku Taira ◽  
Azusa Oshima ◽  
Yasuo Kimura ◽  
Michio Niwano
Keyword(s):  
Lipid Bilayers ◽  
Free Standing ◽  
Nanoporous Alumina ◽  
Alumina Films ◽  
Improved Stability

Measurement of membrane tension of free standing lipid bilayers via laser-induced surface deformation spectroscopy

Soft Matter ◽  
2015 ◽  
Vol 11 (44) ◽  
pp. 8641-8647 ◽  
Author(s):  
Tomohiko Takei ◽  
Tatsuya Yaguchi ◽  
Takuya Fujii ◽  
Tomonori Nomoto ◽  
Taro Toyota ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Surface Deformation ◽  
Membrane Tension ◽  
Free Standing ◽  
Black Lipid Membranes ◽  
Non Invasive ◽  
Invasive Measurement

Non-invasive measurement of the membrane tension of free-standing black lipid membranes (BLMs), with sensitivity on the order of μN m−1, was achieved using laser-induced surface deformation (LISD) spectroscopy.

Free-Standing Lipid Bilayers in Silicon Chips−Membrane Stabilization Based on Microfabricated Apertures with a Nanometer-Scale Smoothness

Langmuir ◽  
2010 ◽  
Vol 26 (3) ◽  
pp. 1949-1952 ◽  
Author(s):  
Ayumi Hirano-Iwata ◽  
Kouji Aoto ◽  
Azusa Oshima ◽  
Tasuku Taira ◽  
Ryo-taro Yamaguchi ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Nanometer Scale ◽  
Membrane Stabilization ◽  
Free Standing ◽  
Silicon Chips

scholarly journals DNA-Machinery for Delivering Membrane Proteins into Free Standing Lipid Bilayers

2011 ◽  
Vol 100 (3) ◽  
pp. 633a
Author(s):  
Minsub Chung ◽  
Poul Martin Bendix ◽  
Namdoo Kim ◽  
Steven G. Boxer
Keyword(s):  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Free Standing

Free-Standing Lipid Bilayers Based on Nanopore Array and Ion Channel Formation

2019 ◽  
Vol 19 (11) ◽  
pp. 7149-7155
Author(s):  
Shengwei Tan ◽  
Ling Zhang ◽  
Lijuan Yu ◽  
Lei Xu
Keyword(s):  
Silicon Nitride ◽  
Ion Channel ◽  
Lipid Bilayers ◽  
Single Molecule ◽  
Protein Function ◽  
Lipid Membranes ◽  
Membrane Resistance ◽  
Label Free ◽  
Free Standing ◽  
Membrane Lifetime

Integrated nanopores are novel and versatile single-molecule sensors for individual label-free biopolymer detection and characterization. However, their studies and application requires a stable lipid bilayer to maintain protein function. Herein, we describe a method for producing lipid bilayers across a nanopore array on a silicon nitride substrate. We used a painting technique commonly used with Teflon films to embed α-hemolysin (α-HL) into bilayer lipid membranes (BLMs) to form an ion channel. This was carried out in nanofluid developed in our lab. The membrane formation process, stability of BLMs and ion channel recordings were monitored by patch clamp in real-time. BLM formation was demonstrated by electrical recording (<10 pS conductance) of suspended lipid bilayers spanning a nanopore in the range of ±100 mV. Membrane resistance (Rm) and capacitance (Cm) of the device with the bilayer were assessed by membrane test as above 1.0 GΩ and ~20±2 pF, respectively. The silicon nitride surface and aperture edge were smooth at the nanometer lever leading to remarkable membrane stability. The membrane lifetime was 5–24 h. A single α-HL channel inserted in 30–60 min applied a potential of +100 mV. The α-HL channel currents were recorded at ~100±10 pA. Such integrated nanopores enable analysis of channel functions under various solution conditions from the same BLM. This will open up a variety of applications for ion channels including high-throughput medical screening and diagnosis.

scholarly journals Theoretical and computational modeling of the dynamics of multicellular and lipid membrane systems

2016 ◽  
Author(s):  
◽  
Matthew McCune
Keyword(s):  
Computational Modeling ◽  
Neutron Scattering ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Drug Testing ◽  
Lipid Membrane ◽  
Dynamic Properties ◽  
Practical Implication ◽  
Free Standing ◽  
Silica Substrate

This dissertation presents two research projects that apply theoretical and computational modeling to (1) describe and predict the formation and shape evolution of three-dimensional (3D) bioprinted tissue constructs, and (2) investigate the effect of a silica substrate on the structural and dynamic properties of a single fully hydrated lipid bilayer. (1) Bioprinting, a novel tissue engineering technique, has the ultimate goal of using 3D printers with bioink made from a person’s own cells to create tissues in the laboratory for transplantation or drug testing. The outcome of the post-bioprinting process, where the bioink particles fuse to form the desired 3D tissue construct, is difficult to predict and experimental techniques have generally been optimized through trial and error. To address this shortcoming, by employing theoretical modeling and computer simulations, we have developed and implemented an effective procedure that is capable of describing and predicting the shape dynamics during post-printing structure formation in 3D bioprinting. In particular, we have explained and demonstrated that the post-printing fusion process is considerably faster when using cylindrical instead of spheroidal bioink particles, a result that has considerable practical implication for extrusion bioprinting. (2) The study of lipid bilayers using neutron scattering experiments requires samples that contain a large stack of membranes. The analysis and computer simulation of such systems is challenging mainly due to the unknown amount of water separating the membranes. To overcome this difficulty, more recent experiments place single lipid membranes onto a support and stack about a hundred of them together. In this project we use molecular dynamics simulations of both free-standing and hydrated single-supported lipid bilayers to investigate the effect of the silica substrate on the structural and dynamical properties of the lipids and hydration waters. Our results may provide useful information in interpreting some recent neutron scattering experiments.

scholarly journals Novel Pre-oxidation Patterning on Thin Aluminium Film Generating Ordered Nanopores through Anodization

2010 ◽  
Vol 1258 ◽  
Author(s):  
Giovanni Fois ◽  
Ciara Therese Bolger ◽  
Justin D Holmes ◽  
Graham Cross
Keyword(s):  
Thin Films ◽  
Silicon Substrates ◽  
Free Standing ◽  
Contact Mode ◽  
Interpore Distance ◽  
Self Organized ◽  
Wide Range ◽  
Anodization Process ◽  
Nanopore Arrays ◽  
Aluminium Film

AbstractAnodic Aluminum Oxide (AAO) is widely employed as a template for fabrication of nanowires and nanotubes due to its ability to generate self organized (SO), well ordered pore structures. We have developed a new aluminum pre-patterning technique to create well ordered nanopore arrays on thin films deposited on silicon substrates. We form patterns of thicker oxide on the surface via local oxidation process using a conducting Atomic Force Microscope (AFM) tip working in contact mode. Pores are forced to nucleate between the pre-oxidized regions during the anodization process. The relation between applied voltage and ordered interpore distance has been found to be linear for these supported thin films. However, the pore spacing is highly reduced compared to free standing foils. A new empiric law has been confirmed for a wide range of voltages, solution concentrations and different electrolytes, including oxalic and phosphoric acid. Our results show that pre-oxidation patterning is an alternative technique to achieve an ordered nanoporous template through the anodization process.

Sign in / Sign up

Export Citation Format

Share Document