scholarly journals Bone Substitutes: 3D Printed Porous Methacrylate/Silica Hybrid Scaffold for Bone Substitution (Adv. Healthcare Mater. 12/2021)

2021 ◽  
Vol 10 (12) ◽  
pp. 2170058
Author(s):  
Justin J. Chung ◽  
Jin Yoo ◽  
Brian S. T. Sum ◽  
Siwei Li ◽  
Soojin Lee ◽  
...  
Keyword(s):  
Bone Substitutes ◽  
Hybrid Scaffold ◽  
3D Printed ◽  
Silica Hybrid

Bioresorption and biodegradation of the 3D-printed gene-activated bone substitutes based on octacalcium phosphate

2020 ◽  
Vol XV (1) ◽  
Author(s):  
E. Presnyakov ◽  
I. Bozo ◽  
I. Smirnov ◽  
V. Komlev ◽  
V. Popov ◽  
...  
Keyword(s):  
Bone Substitutes ◽  
Octacalcium Phosphate ◽  
3D Printed

scholarly journals Total Meniscus Reconstruction Using a Polymeric Hybrid-Scaffold: Combined with 3D-Printed Biomimetic Framework and Micro-Particle

Polymers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1910
Author(s):  
Hun-Jin Jeong ◽  
Se-Won Lee ◽  
Myoung Wha Hong ◽  
Young Yul Kim ◽  
Kyoung Duck Seo ◽  
...  
Keyword(s):  
Shape Matching ◽  
Femoral Condyle ◽  
Immunohistochemical Analysis ◽  
Cartilage Degeneration ◽  
Patient Specific ◽  
Hybrid Scaffold ◽  
Meniscus Tissue ◽  
Cell Source ◽  
3D Printed ◽  
Meniscus Regeneration

The meniscus has poor intrinsic regenerative capability, and its injury inevitably leads to articular cartilage degeneration. Although there are commercialized off-the-shelf alternatives to achieve total meniscus regeneration, each has its own shortcomings such as individualized size matching issues and inappropriate mechanical properties. We manufactured a polycaprolactone-based patient-specific designed framework via a Computed Tomography scan images and 3D-printing technique. Then, we completed the hybrid-scaffold by combining the 3D-printed framework and mixture micro-size composite which consists of polycaprolactone and sodium chloride to create a cell-friendly microenvironment. Based on this hybrid-scaffold with an autograft cell source (fibrochondrocyte), we assessed mechanical and histological results using the rabbit total meniscectomy model. At postoperative 12-week, hybrid-scaffold achieved neo-meniscus tissue formation, and its shape was maintained without rupture or break away from the knee joint. Histological and immunohistochemical analysis results showed obvious ingrowth of the fibroblast-like cells and chondrocyte cells as well as mature lacunae that were embedded in the extracellular matrix. Hybrid-scaffolding resulted in superior shape matching as compared to original meniscus tissue. Histological analysis showed evidence of extensive neo-meniscus cell ingrowth. Additionally, the hybrid-scaffold did not induce osteoarthritis on the femoral condyle surface. The 3D-printed hybrid-scaffold may provide a promising approach that can be applied to those who received total meniscal resection, using patient-specific design and autogenous cell source.

scholarly journals 3D Printed Gene-activated Octacalcium Phosphate Implants for Large Bone Defects Engineering

2020 ◽  
Vol 6 (3) ◽  
Author(s):  
Ilya I Bozo ◽  
Roman V. Deev ◽  
Igor V. Smirnov ◽  
Alexander Yu Fedotov ◽  
Vladimir K. Popov ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Three Dimensional ◽  
Bone Substitutes ◽  
Octacalcium Phosphate ◽  
Dna Encoding ◽  
X Ray Diffraction ◽  
3D Printed ◽  
Clinical Potential ◽  
Large Bone

The aim of the study was the development of three-dimensional (3D) printed gene-activated implants based on octacalcium phosphate (OCP) and plasmid DNA encoding VEGFA. The first objective of the present work involved design and fabrication of gene-activated bone substitutes based on the OCP and plasmid DNA with VEGFА gene using 3D printing approach of ceramic constructs, providing the control of its architectonics compliance to the initial digital models. X-ray diffraction, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and compressive strength analyses were applied to investigate the chemical composition, microstructure, and mechanical properties of the experimental samples. The biodegradation rate and the efficacy of plasmid DNA delivery in vivo were assessed during standard tests with subcutaneous implantation to rodents in the next stage. The final part of the study involved substitution of segmental tibia and mandibular defects in adult pigs with 3D printed gene-activated implants. Biodegradation, osteointegration, and effectiveness of a reparative osteogenesis were evaluated with computerized tomography, SEM, and a histological examination. The combination of gene therapy and 3D printed implants manifested the significant clinical potential for effective bone regeneration in large/critical size defect cases.

scholarly journals Enzyme degradable star polymethacrylate/silica hybrid inks for 3D printing of tissue scaffolds

2020 ◽  
Vol 1 (9) ◽  
pp. 3189-3199
Author(s):  
Anna Li Volsi ◽  
Francesca Tallia ◽  
Haffsah Iqbal ◽  
Theoni K. Georgiou ◽  
Julian R. Jones
Keyword(s):  
3D Printing ◽  
Bone Regeneration ◽  
Tissue Scaffolds ◽  
Star Polymer ◽  
Polymer Architecture ◽  
Organic Hybrid ◽  
3D Printed ◽  
Silica Hybrid

We report the first enzyme cleavable inorganic–organic hybrid “inks” that can be 3D printed as scaffolds for bone regeneration and investigate the effect of star polymer architecture on their properties.

scholarly journals A Stereolithography-Based 3D Printed Hybrid Scaffold for In Situ Cartilage Defect Repair

2017 ◽  
Vol 18 (2) ◽  
pp. 1700267 ◽  
Author(s):  
Elizabeth A. Aisenbrey ◽  
Andrew Tomaschke ◽  
Eric Kleinjan ◽  
Archish Muralidharan ◽  
Cecilia Pascual-Garrido ◽  
...  
Keyword(s):  
Cartilage Defect ◽  
Hybrid Scaffold ◽  
Cartilage Defect Repair ◽  
Defect Repair ◽  
3D Printed

scholarly journals Fabrication and Application of a 3D-Printed Poly-ε-Caprolactone Cage Scaffold for Bone Tissue Engineering

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Siyi Wang ◽  
Rong Li ◽  
Yongxiang Xu ◽  
Dandan Xia ◽  
Yuan Zhu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Synthetic Material ◽  
Hybrid Scaffold ◽  
Natural Bone Mineral ◽  
Application Potential ◽  
3D Printed

Poly-ε-caprolactone (PCL) is a promising synthetic material in bone tissue engineering (BTE). Particularly, the introduction of rapid prototyping (RP) represents the possibility of manufacturing PCL scaffolds with customized appearances and structures. Bio-Oss is a natural bone mineral matrix with significant osteogenic effects; however, it has limitations in being constructed and maintained into specific shapes and sites. In this study, we used RP and fabricated a hollow-structured cage-shaped PCL scaffold loaded with Bio-Oss to form a hybrid scaffold for BTE. Moreover, we adopted NaOH surface treatment to improve PCL hydrophilicity and enhance cell adhesion. The results showed that the NaOH-treated hybrid scaffold could enhance the osteogenesis of human bone marrow-derived mesenchymal stem cells (hBMMSCs) both in vitro and in vivo. Altogether, we reveal a novel hybrid scaffold that not only possesses osteoinductive function to promote bone formation but can also be fabricated into specific forms. This scaffold design may have great application potential in bone tissue engineering.

scholarly journals MECHANICAL PROPERTIES OF 3D SCAFFOLDS FOR BONE REGENERATION / 3D KARKASŲ, SKIRTŲ KAULŲ REGENERACIJAI, MECHANINIŲ SAVYBIŲ TYRIMAS

2017 ◽  
Vol 8 (6) ◽  
pp. 587-591
Author(s):  
Deividas Mizeras ◽  
Andžela Šešok ◽  
Algirdas Vaclovas Valiulis ◽  
Justinas Gargasas ◽  
Irmantas Gedzevičius
Keyword(s):  
Mechanical Properties ◽  
Testing Machine ◽  
Bone Substitutes ◽  
Point Of View ◽  
Bone Tissue Regeneration ◽  
3D Scaffolds ◽  
Bending Tests ◽  
Ethical Point ◽  
Additional Surgery ◽  
3D Printed

One of the biggest challenges in modern tissue engineering is a creation 3D scaffolds for bone tissue regeneration. Until now, in order to restore bone defects are used various bone substitutes (autologous and allogeneic), however, their usage is limited because is required additional surgery, possible complications, also limited their use is associated with ethical point of view. In this work we aim to determine the mechanical properties of 3D printed PLA objects having various orientation woodpile microarchitectures. In this work we chose three different 3D microarchitectures: woodpile BCC (each layer consists of parallel logs which are rotated 90 deg every next layer), woodpile FCC (every layer is additionally shifted half of the period in respect to the previous parallel log layer) and a rotating woodpile 60 deg (each layer is rotated 60 deg in respect to the previous one). Compressive and bending tests were carried out with TIRAtest2300 universal testing machine. We found that 60 deg rotating woodpile geometry had the highest mechanical values which were approximately about 3 times higher than the BCC or FCC microstructures. Vienas didžiausių šiuolaikinės audinių inžinerijos iššūkių yra 3D karkasų, skirtų kaulinio audinio regeneracijai, sukūrimas. Iki šiol, norint atstatyti kaulo defektus, naudojami įvairūs kaulo pakaitalai (autogeniniai ir alogeniniai), kurių naudojimo galimybės jau nebeatitinka poreikių, nes reikalinga papildoma operacija, galimos komplikacijos, taip pat ribotas jų naudojimas, susijęs su etinėmis pažiūromis. Šiame darbe lyginamos 3D spausdintuvu suformuotų mikrodarinių, skirtų kaulinio audinio defektui atkurti, mechaninės savybės. Darbe pasirinktos trys skirtingos 3D karkasų mikrostruktūros: woodpile BCC (kiekvienas sluoksnis susideda iš lygiagrečių rąstų, kurie keičiami 90 laipsnių kampu prieš tai esančio sluoksnio atžvilgiu), woodpile FCC (kiekvienas sluoksnis papildomai keičiasi per pusę periodo sluoksnio, esančio prieš tai, atžvilgiu) ir woodpile 60 deg (besisukanti rąstų rietuvė, kiekvienas tokios gardelės sluoksnis yra pasuktas 60 laipsnių prieš tai esančios atžvilgiu). Gniuždymo ir lenkimo bandymai buvo atlikti TIRAtest 2300 universalia bandymų mašina. Buvo nustatyta, kad, taikant 60 laipsnių kampu besikeičiančią woodpile geometriją, galima pasiekti didžiausias mechanines vertes, kurios buvo maždaug tris kartus didesnės nei woodfile BCC arba woodfile FCCmikrostruktūros.

3D Printed Multifunctional Ti6Al4V-Based Hybrid Scaffold for the Management of Osteosarcoma

2021 ◽  
Author(s):  
Bianyun Cai ◽  
Leizhen Huang ◽  
Jingcheng Wang ◽  
Dan Sun ◽  
Ce Zhu ◽  
...  
Keyword(s):  
Hybrid Scaffold ◽  
3D Printed

scholarly journals Microporosities in 3D-Printed Tricalcium-Phosphate-Based Bone Substitutes Enhance Osteoconduction and Affect Osteoclastic Resorption

2020 ◽  
Vol 21 (23) ◽  
pp. 9270
Author(s):  
Chafik Ghayor ◽  
Tse-Hsiang Chen ◽  
Indranil Bhattacharya ◽  
Mutlu Özcan ◽  
Franz E. Weber
Keyword(s):  
Additive Manufacturing ◽  
Tricalcium Phosphate ◽  
Major Complication ◽  
Bone Substitutes ◽  
In Vivo Studies ◽  
Patient Specific ◽  
Osteoclastic Resorption ◽  
3D Printed ◽  
High Degree

Additive manufacturing is a key technology required to realize the production of a personalized bone substitute that exactly meets a patient’s need and fills a patient-specific bone defect. Additive manufacturing can optimize the inner architecture of the scaffold for osteoconduction, allowing fast and reliable defect bridging by promoting rapid growth of new bone tissue into the scaffold. The role of scaffold microporosity/nanoarchitecture in osteoconduction remains elusive. To elucidate this relationship, we produced lithography-based osteoconductive scaffolds from tricalcium phosphate (TCP) with identical macro- and microarchitecture, but varied their nanoarchitecture/microporosity by ranging maximum sintering temperatures from 1000 °C to 1200 °C. After characterization of the different scaffolds’ microporosity, compression strength, and nanoarchitecture, we performed in vivo studies that showed that ingrowth of bone as an indicator of osteoconduction significantly decreased with decreasing microporosity. Moreover, at the 1200 °C peak sinter temperature and lowest microporosity, osteoclastic degradation of the material was inhibited. Thus, even for wide-open porous TCP-based scaffolds, a high degree of microporosity appears to be essential for optimal osteoconduction and creeping substitution, which can prevent non-unions, the major complication during bone regeneration procedures.

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