scholarly journals A Stereolithography-Based 3D Printed Hybrid Scaffold for In Situ Cartilage Defect Repair

2017 ◽  
Vol 18 (2) ◽  
pp. 1700267 ◽  
Author(s):  
Elizabeth A. Aisenbrey ◽  
Andrew Tomaschke ◽  
Eric Kleinjan ◽  
Archish Muralidharan ◽  
Cecilia Pascual-Garrido ◽  
...  
Keyword(s):  
Cartilage Defect ◽  
Hybrid Scaffold ◽  
Cartilage Defect Repair ◽  
Defect Repair ◽  
3D Printed

scholarly journals Advanced Strategies for Articular Cartilage Defect Repair

Materials ◽  
2013 ◽  
Vol 6 (2) ◽  
pp. 637-668 ◽  
Author(s):  
Amos Matsiko ◽  
Tanya Levingstone ◽  
Fergal O'Brien
Keyword(s):  
Articular Cartilage ◽  
Cartilage Defect ◽  
Articular Cartilage Defect ◽  
Cartilage Defect Repair ◽  
Defect Repair

scholarly journals Chitosan composite scaffolds for articular cartilage defect repair: a review

RSC Advances ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 3736-3749 ◽  
Author(s):  
Huijun Li ◽  
Cheng Hu ◽  
Huijun Yu ◽  
Chuanzhong Chen
Keyword(s):  
Articular Cartilage ◽  
Cartilage Defect ◽  
Composite Scaffolds ◽  
Articular Cartilage Defect ◽  
Cartilage Defect Repair ◽  
Defect Repair ◽  
Chitosan Composite

Properties of chitosan/polymer and chitosan/bioceramic composite scaffolds for articular cartilage defect repair are reviewed.

Chondrocyte cell-based techniques for cartilage defect repair

2011 ◽  
Vol 22 (4) ◽  
pp. 339-343
Author(s):  
Stefan Schüttler ◽  
Nenad Andjelkov
Keyword(s):  
Cartilage Defect ◽  
Cartilage Defect Repair ◽  
Defect Repair

scholarly journals Hyaluronic acid hydrogel encapsulated BMP-14-modified ADSCs accelerate cartilage defect repair in rabbits

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Hao Liu ◽  
Yongjun Rui ◽  
Jun Liu ◽  
Fandong Gao ◽  
Yesheng Jin
Keyword(s):  
Cartilage Defect ◽  
Adenovirus Vector ◽  
Rt Pcr ◽  
Differentiation Markers ◽  
Western Blot Assay ◽  
Transfection Efficacy ◽  
Cartilage Defect Repair ◽  
Defect Repair ◽  
Collagen Ii

Abstract Background Cartilage defect has a limited capacity to heal. In this context, we hypothesized that hyaluronic acid (HA) hydrogel encapsulated BMP-14-modified adipose-derived mesenchymal stem cells (ADSCs) could accelerate cartilage defect repair in rabbits. Methods ADSCs were isolated and identified by flow cytometry. ADSCs were treated with adenovirus vector encoding BMP-14 (Ad-BMP-14) or adenovirus vector encoding control (Ad-ctrl). Real-time PCR (RT-PCR) and western blot assay was performed to verify the transfection efficacy and chondrogenic differentiation markers (ACAN, Collagen II and SOX9). Rabbit cartilage defect model was performed and randomly divided into following groups: control group, HA hydrogel + ADSCs, ADSCs, HA hydrogel + BMP-14 transfected ADSCs, HA hydrogel + BMP-14 transfected ADSCs. At 6, 9 and 12 weeks after surgery, scanning electron microscopy, hematoxylin–eosin, Safranin-O/Fast Green and immunohistochemical staining for Collagen II were performed to determine the role of HA hydrogel encapsulated BMP-14-modified ADSCs in cartilage repair in vivo. Results ADSCs were successfully isolated and positively expressed CD29, CD44 and CD90. Transfection efficacy of Ad-BMP-14 was verified by RT-PCR and western blot assay. Moreover, Ad-BMP-14 could significantly increased chondrogenic differentiation markers (ACAN, Collagen II and SOX9). The LV-BMP-14-ADSCs and HA hydrogel + LV-BMP-14-ADSCs groups revealed smoother surface cartilage repair that was level with the surrounding cartilage and almost complete border integration. Conclusions HA hydrogel encapsulated BMP-14-modified ADSCs accelerate cartilage defect repair in rabbits. We need to further validate the specific mechanism of action of HA hydrogel encapsulated LV-BMP-14-ADSCs involved in the repairing cartilage damage in vivo.

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