scholarly journals Human Neural Tissues from Neural Stem Cells Using Conductive Biogel and Printed Polymer Microelectrode Arrays for 3D Electrical Stimulation

2019 ◽  
Vol 8 (15) ◽  
pp. 1900425 ◽  
Author(s):  
Eva Tomaskovic‐Crook ◽  
Peikai Zhang ◽  
Annika Ahtiainen ◽  
Heidi Kaisvuo ◽  
Chong‐Yong Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Electrical Stimulation ◽  
Neural Stem Cells ◽  
Microelectrode Arrays ◽  
Neural Tissues

scholarly journals Enriching neural stem cell and pro-healing glial phenotypes with electrical stimulation after traumatic brain injury in male rats

2020 ◽  
Author(s):  
Eunyoung Park ◽  
Johnathan G. Lyon ◽  
Melissa Alvarado-Velez ◽  
Martha I. Betancur ◽  
Nassir Mokarram ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Stem Cells ◽  
Electrical Stimulation ◽  
Brain Injury ◽  
Neural Stem Cells ◽  
Acute Phase ◽  
Immune Cells ◽  
Male Rats ◽  
Cell Phenotype ◽  
Injury Site

AbstractTraumatic Brain Injury (TBI) by an external physical impact results in compromised brain function via undesired neuronal death. Following the injury, resident and peripheral immune cells, astrocytes, and neural stem cells (NSCs) cooperatively contribute to the recovery of the neuronal function after TBI. However, excessive pro-inflammatory responses of immune cells, and the disappearance of endogenous NSCs at the injury site during the acute phase of TBI, can exacerbate TBI progression leading to incomplete healing. Therefore, positive outcomes may depend on early interventions to control the injury-associated cellular milieu in the early phase of injury. Here, we explore electrical stimulation (ES) of the injury site in a rodent model (male Sprague-Dawley rats) to investigate its overall effect on the constituent brain cell phenotype and composition during the acute phase of TBI. Our data showed that a brief ES for 1h on day 2 of TBI promoted pro-healing phenotypes of microglia as assessed by CD206 expression and increased the population of NSCs and Nestin+ astrocytes at 7 days post-TBI. Also, ES effectively increased the number of viable neurons when compared to the unstimulated control group. Given the salience of microglia and neural stem cells for healing after TBI, our results strongly support the potential benefit of the therapeutic use of ES during the acute phase of TBI to regulate neuroinflammation and to enhance neuroregeneration.Significance StatementTraumatic brain injury (TBI) occurs when a head injury leads to a disruption of normal function in the brain and is a major cause of death and disability, worldwide. The authors used electrical stimulation during the acute phase of TBI, which promoted pro-healing phenotypes of microglia and increased the number of neural stem cells and Nestin+ astrocytes, thereby enhancing neuronal viability. These findings support further study of electrical stimulation to regulate neuroinflammation and to enhance neuroregeneration after TBI.Graphical AbstractFIGURE 1.

A micropatterned conductive electrospun nanofiber mesh combined with electrical stimulation for synergistically enhancing differentiation of rat neural stem cells

2020 ◽  
Vol 8 (13) ◽  
pp. 2673-2688 ◽  
Author(s):  
Huanhuan Yan ◽  
Yu Wang ◽  
Linlong Li ◽  
Xiaosong Zhou ◽  
Xincui Shi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Electrical Stimulation ◽  
Neural Stem Cells ◽  
Electrospun Nanofiber ◽  
Nanofiber Mesh

The micropatterned conductive nanofiber mesh combined with ES effectively facilitates the differentiation of NSCs into neuron and suppresses the formation of astrocytes.

Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere Technique

2010 ◽  
Vol 15 (1) ◽  
Author(s):  
Daniela Ferrari ◽  
Elena Binda ◽  
Lidia De Filippis ◽  
Angelo Luigi Vescovi
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Neural Tissues

scholarly journals Electrical Stimulation Elicits Neural Stem Cells Activation: New Perspectives in CNS Repair

2015 ◽  
Vol 9 ◽  
Author(s):  
Yanhua Huang ◽  
YeE Li ◽  
Jian Chen ◽  
Hongxing Zhou ◽  
Sheng Tan
Keyword(s):  
Stem Cells ◽  
Electrical Stimulation ◽  
Neural Stem Cells ◽  
Cns Repair
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