Heparin‐Conjugated Decellularized Bone Particles Promote Enhanced Osteogenic Signaling of PDGF‐BB to Adipose‐Derived Stem Cells in Tissue Engineered Bone Grafts

2019 ◽  
Vol 8 (10) ◽  
pp. 1801565 ◽  
Author(s):  
Alexandra N. Rindone ◽  
Bartlomiej Kachniarz ◽  
Chukwuebuka C. Achebe ◽  
Ryan C. Riddle ◽  
Aine N. O'Sullivan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Grafts ◽  
Adipose Derived Stem Cells ◽  
Bone Particles ◽  
Osteogenic Signaling

scholarly journals Bone Grafts Engineered from Human Adipose-Derived Stem Cells in Perfusion Bioreactor Culture

2010 ◽  
Vol 16 (1) ◽  
pp. 179-189 ◽  
Author(s):  
Mirjam Fröhlich ◽  
Warren L. Grayson ◽  
Darja Marolt ◽  
Jeffrey M. Gimble ◽  
Nevenka Kregar-Velikonja ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Grafts ◽  
Perfusion Bioreactor ◽  
Bioreactor Culture ◽  
Adipose Derived Stem Cells

scholarly journals Tissue engineered bone grafts based on biomimetic nanocomposite PLGA/amorphous calcium phosphate scaffold and human adipose-derived stem cells

Injury ◽  
2012 ◽  
Vol 43 (10) ◽  
pp. 1689-1697 ◽  
Author(s):  
Johanna Buschmann ◽  
Luc Härter ◽  
Shuping Gao ◽  
Sonja Hemmi ◽  
Manfred Welti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Bone Grafts ◽  
Adipose Derived Stem Cells

Bone grafts engineered from human adipose-derived stem cells in dynamic 3D-environments

Biomaterials ◽  
2013 ◽  
Vol 34 (4) ◽  
pp. 1004-1017 ◽  
Author(s):  
Heidi A. Declercq ◽  
Tamara De Caluwé ◽  
Olga Krysko ◽  
Claus Bachert ◽  
Maria J. Cornelissen
Keyword(s):  
Stem Cells ◽  
Bone Grafts ◽  
Adipose Derived Stem Cells ◽  
3D Environments

scholarly journals Electrical Stimulation of Adipose-Derived Stem Cells in 3D Nanofibrillar Cellulose Increases Their Osteogenic Potential

Biomolecules ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1696
Author(s):  
Mesude Bicer ◽  
Jonathan Sheard ◽  
Donata Iandolo ◽  
Samuel Y. Boateng ◽  
Graeme S. Cottrell ◽  
...  
Keyword(s):  
Stem Cells ◽  
Electrical Stimulation ◽  
Osteoporotic Fractures ◽  
Bone Grafts ◽  
Osteogenic Potential ◽  
Ageing Population ◽  
Adipose Derived Stem Cells ◽  
Alizarin Red ◽  
Nanofibrillar Cellulose ◽  
Biophysical Cues

Due to the ageing population, there is a steadily increasing incidence of osteoporosis and osteoporotic fractures. As conventional pharmacological therapy options for osteoporosis are often associated with severe side effects, bone grafts are still considered the clinical gold standard. However, the availability of viable, autologous bone grafts is limited making alternative cell-based strategies a promising therapeutic alternative. Adipose-derived stem cells (ASCs) are a readily available population of mesenchymal stem/stromal cells (MSCs) that can be isolated within minimally invasive surgery. This ease of availability and their ability to undergo osteogenic differentiation makes ASCs promising candidates for cell-based therapies for bone fractures. Recent studies have suggested that both exposure to electrical fields and cultivation in 3D can positively affect osteogenic potential of MSCs. To elucidate the osteoinductive potential of a combination of these biophysical cues on ASCs, cells were embedded within anionic nanofibrillar cellulose (aNFC) hydrogels and exposed to electrical stimulation (ES) for up to 21 days. ES was applied to ASCs in 2D and 3D at a voltage of 0.1 V/cm with a duration of 0.04 ms, and a frequency of 10 Hz for 30 min per day. Exposure of ASCs to ES in 3D resulted in high alkaline phosphatase (ALP) activity and in an increased mineralisation evidenced by Alizarin Red S staining. Moreover, ES in 3D aNFC led to an increased expression of the osteogenic markers osteopontin and osteocalcin and a rearrangement and alignment of the actin cytoskeleton. Taken together, our data suggest that a combination of ES with 3D cell culture can increase the osteogenic potential of ASCs. Thus, exposure of ASCs to these biophysical cues might improve the clinical outcomes of regenerative therapies in treatment of osteoporotic fractures.

Osteoplasty in Disseminated Calvarial Defects Treated with Autologous Adipose-Derived Stem Cells and Autologous Fibrin Glue: Case Report

Skull Base ◽  
2005 ◽  
Vol 15 (S 2) ◽  
Author(s):  
Stefan Lendeckel ◽  
A. Jödicke ◽  
P. Christophis ◽  
K. Heidinger ◽  
H.-P. Howaldt
Keyword(s):  
Stem Cells ◽  
Case Report ◽  
Fibrin Glue ◽  
Adipose Derived Stem Cells ◽  
Autologous Fibrin Glue ◽  
Calvarial Defects
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